Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
Background/Aims: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMC...
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| Veröffentlicht in: | The Korean journal of internal medicine 2008-12, Vol.23 (4), p.176 |
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| creator | Inseon S. Choi Yong Cui Young Ah Koh Hyun Chul Lee Yong Bum Cho Young Ho Won |
| description | Background/Aims: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. Methods: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 μM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. Results: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p |
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Choi ; Yong Cui ; Young Ah Koh ; Hyun Chul Lee ; Yong Bum Cho ; Young Ho Won</creator><creatorcontrib>Inseon S. Choi ; Yong Cui ; Young Ah Koh ; Hyun Chul Lee ; Yong Bum Cho ; Young Ho Won</creatorcontrib><description>Background/Aims: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. Methods: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 μM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. Results: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 μM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. Conclusions: DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.</description><identifier>ISSN: 1226-3303</identifier><identifier>EISSN: 2005-6648</identifier><language>kor</language><publisher>대한내과학회</publisher><subject>Asthma ; Atopy ; Dehydroepiandrosterone ; Mononuclear cells ; Th2</subject><ispartof>The Korean journal of internal medicine, 2008-12, Vol.23 (4), p.176</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Inseon S. Choi</creatorcontrib><creatorcontrib>Yong Cui</creatorcontrib><creatorcontrib>Young Ah Koh</creatorcontrib><creatorcontrib>Hyun Chul Lee</creatorcontrib><creatorcontrib>Yong Bum Cho</creatorcontrib><creatorcontrib>Young Ho Won</creatorcontrib><title>Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics</title><title>The Korean journal of internal medicine</title><addtitle>The Korean Journal of Internal Medicine</addtitle><description>Background/Aims: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. Methods: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 μM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. Results: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 μM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. Conclusions: DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.</description><subject>Asthma</subject><subject>Atopy</subject><subject>Dehydroepiandrosterone</subject><subject>Mononuclear cells</subject><subject>Th2</subject><issn>1226-3303</issn><issn>2005-6648</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9ictqwzAQAEVoIabNF_SyP2DQI3Z6Dyn9gNyDIq-wiKwVu8rBfx8feu5pmJmd6qzWQz-Ox-831Rlrx9457fbqIJLuWjtjRqOHTvElRgxNgCJMOK8TE9bky0ZpyFQQqMB1thDWRo-0eWWanqGlracCFTnVGdlnuGeiCRYqVJ4ho2cImLNAZFrAS5sX31KQT_UefRY8_PFDff1cruff_pFEbpXT4nm92dNJm0G7_-8LJ41JIQ</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Inseon S. Choi</creator><creator>Yong Cui</creator><creator>Young Ah Koh</creator><creator>Hyun Chul Lee</creator><creator>Yong Bum Cho</creator><creator>Young Ho Won</creator><general>대한내과학회</general><scope>HZB</scope><scope>Q5X</scope></search><sort><creationdate>20081201</creationdate><title>Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics</title><author>Inseon S. Choi ; Yong Cui ; Young Ah Koh ; Hyun Chul Lee ; Yong Bum Cho ; Young Ho Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kiss_primary_27701503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2008</creationdate><topic>Asthma</topic><topic>Atopy</topic><topic>Dehydroepiandrosterone</topic><topic>Mononuclear cells</topic><topic>Th2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inseon S. Choi</creatorcontrib><creatorcontrib>Yong Cui</creatorcontrib><creatorcontrib>Young Ah Koh</creatorcontrib><creatorcontrib>Hyun Chul Lee</creatorcontrib><creatorcontrib>Yong Bum Cho</creatorcontrib><creatorcontrib>Young Ho Won</creatorcontrib><collection>Korean Studies Information Service System (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><jtitle>The Korean journal of internal medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inseon S. Choi</au><au>Yong Cui</au><au>Young Ah Koh</au><au>Hyun Chul Lee</au><au>Yong Bum Cho</au><au>Young Ho Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics</atitle><jtitle>The Korean journal of internal medicine</jtitle><addtitle>The Korean Journal of Internal Medicine</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>23</volume><issue>4</issue><spage>176</spage><pages>176-</pages><issn>1226-3303</issn><eissn>2005-6648</eissn><abstract>Background/Aims: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. Methods: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 μM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. Results: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 μM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. Conclusions: DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.</abstract><pub>대한내과학회</pub><tpages>6</tpages></addata></record> |
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| ispartof | The Korean journal of internal medicine, 2008-12, Vol.23 (4), p.176 |
| issn | 1226-3303 2005-6648 |
| language | kor |
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| source | KoreaMed Synapse; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Asthma Atopy Dehydroepiandrosterone Mononuclear cells Th2 |
| title | Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics |
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