암모니아로 인한 위점막 손상에 대한 Glutathione 의 역할 및 Cysteamine 의 보호 효과 －Helicobacter pylori 의 위장질환 병인론과 관련하여

Background/Aims: The mechanism of gastric mucosal injury by ammonia, one of the toxic products of Helicobacter pylori, is still unclear. We investigated the role and protective effect of glutathione in the ammonia-induced gastric mucosal injury. Methods: The male Sprague Dawley rats were sacrified after intragastric administration of 1 ml of 1% ammonia with pretreating intragastric cysteamine (Cys-am group), or intraperitoneal buthionine sulfoximine (BSO-am group), or without any pretreatment (Cont-am group). Additional groups of rats were pretreated with equivalent cysteamine (Cys group) or eqivalent BSO (BSO group) and without any treatment (Cont group) and then, sacrified. The gastric mucosal glutathione (GSH) and malondialdehyde (MDA) were measured and the severity of gastric mucosal damage was evaluated microscopically. Results: Gastric mucosa GSH was considerably increased in the Cys group and the Cys-am group (p