Inhibition of Lipase Activity in Antibiotic-Resistant Propionibacterium acnes Strains
Background and Objective: Erythromycin-sensitive and/or clindamycin-sensitive strains of Propionibacterium acnes show a reduced lipase production at levels below the minimal growth-inhibitory concentration (MIC). The objective of this study was to determine whether erythromycin and clindamycin conce...
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| Veröffentlicht in: | Dermatology (Basel) 2002-01, Vol.205 (3), p.260-264 |
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| creator | Gloor, Max Wasik, Barbara Becker, Andrea Höffler, Ulrich |
| description | Background and Objective: Erythromycin-sensitive and/or clindamycin-sensitive strains of Propionibacterium acnes show a reduced lipase production at levels below the minimal growth-inhibitory concentration (MIC). The objective of this study was to determine whether erythromycin and clindamycin concentrations far below the MIC inhibit lipase production in P. acnes strains resistant to these antibiotics. Methods: Of 42 P. acnes strains, 10 showed an MIC >256 µg/ml for erythromycin. Two strains showed MICs of 0.19 and 0.25 µg/ml, while the MIC of the remaining strains was ≤0.016 µg/ml. Lipase activity was determined up to a concentration of 192 µg/ml by cultivation on spirit blue agar + lipase reagent. The 10 strains whose erythromycin MIC was >256 µg/ml were also tested for lipase inhibition by clindamycin. While this method fails to differentiate between inhibition of lipase production and inhibition of lipase activity, the absence of inhibition of lipase activity rules out inhibition of lipase production. Results: Inhibition of lipolysis by sub-MIC concentrations was demonstrated only for clindamycin in 3 P. acnes strains. However, lipase inhibition was seen only at the dilution level immediately below the MIC. Conclusions: Resistant P. acnes strains with high erythromycin and/or clindamycin MICs can be ruled out to show in vitro inhibition of lipase production at antibiotic concentrations far below the MIC. |
| doi_str_mv | 10.1159/000065856 |
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The objective of this study was to determine whether erythromycin and clindamycin concentrations far below the MIC inhibit lipase production in P. acnes strains resistant to these antibiotics. Methods: Of 42 P. acnes strains, 10 showed an MIC >256 µg/ml for erythromycin. Two strains showed MICs of 0.19 and 0.25 µg/ml, while the MIC of the remaining strains was ≤0.016 µg/ml. Lipase activity was determined up to a concentration of 192 µg/ml by cultivation on spirit blue agar + lipase reagent. The 10 strains whose erythromycin MIC was >256 µg/ml were also tested for lipase inhibition by clindamycin. While this method fails to differentiate between inhibition of lipase production and inhibition of lipase activity, the absence of inhibition of lipase activity rules out inhibition of lipase production. Results: Inhibition of lipolysis by sub-MIC concentrations was demonstrated only for clindamycin in 3 P. acnes strains. However, lipase inhibition was seen only at the dilution level immediately below the MIC. Conclusions: Resistant P. acnes strains with high erythromycin and/or clindamycin MICs can be ruled out to show in vitro inhibition of lipase production at antibiotic concentrations far below the MIC.</description><identifier>ISSN: 1018-8665</identifier><identifier>EISSN: 1421-9832</identifier><identifier>DOI: 10.1159/000065856</identifier><identifier>PMID: 12399674</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Acne Vulgaris - microbiology ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biological and medical sciences ; Clindamycin - pharmacology ; Dermatology ; Drug Resistance, Bacterial ; Erythromycin - pharmacology ; Humans ; Lipase - drug effects ; Medical sciences ; Microbial Sensitivity Tests ; Pharmacology and Treatment ; Pharmacology. Drug treatments ; Propionibacterium acnes ; Propionibacterium acnes - drug effects ; Propionibacterium acnes - enzymology ; Propionibacterium acnes - isolation & purification ; Skin involvement in other diseases. Miscellaneous. General aspects</subject><ispartof>Dermatology (Basel), 2002-01, Vol.205 (3), p.260-264</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><rights>Copyright (c) 2002 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-d8eca8cc7cbd35acb836ec012215a4ae78794e738357bf74e9282941379347cd3</citedby><cites>FETCH-LOGICAL-c416t-d8eca8cc7cbd35acb836ec012215a4ae78794e738357bf74e9282941379347cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2427,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14008929$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12399674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gloor, Max</creatorcontrib><creatorcontrib>Wasik, Barbara</creatorcontrib><creatorcontrib>Becker, Andrea</creatorcontrib><creatorcontrib>Höffler, Ulrich</creatorcontrib><title>Inhibition of Lipase Activity in Antibiotic-Resistant Propionibacterium acnes Strains</title><title>Dermatology (Basel)</title><addtitle>Dermatology</addtitle><description>Background and Objective: Erythromycin-sensitive and/or clindamycin-sensitive strains of Propionibacterium acnes show a reduced lipase production at levels below the minimal growth-inhibitory concentration (MIC). The objective of this study was to determine whether erythromycin and clindamycin concentrations far below the MIC inhibit lipase production in P. acnes strains resistant to these antibiotics. Methods: Of 42 P. acnes strains, 10 showed an MIC >256 µg/ml for erythromycin. Two strains showed MICs of 0.19 and 0.25 µg/ml, while the MIC of the remaining strains was ≤0.016 µg/ml. Lipase activity was determined up to a concentration of 192 µg/ml by cultivation on spirit blue agar + lipase reagent. The 10 strains whose erythromycin MIC was >256 µg/ml were also tested for lipase inhibition by clindamycin. While this method fails to differentiate between inhibition of lipase production and inhibition of lipase activity, the absence of inhibition of lipase activity rules out inhibition of lipase production. Results: Inhibition of lipolysis by sub-MIC concentrations was demonstrated only for clindamycin in 3 P. acnes strains. However, lipase inhibition was seen only at the dilution level immediately below the MIC. Conclusions: Resistant P. acnes strains with high erythromycin and/or clindamycin MICs can be ruled out to show in vitro inhibition of lipase production at antibiotic concentrations far below the MIC.</description><subject>Acne Vulgaris - microbiology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Clindamycin - pharmacology</subject><subject>Dermatology</subject><subject>Drug Resistance, Bacterial</subject><subject>Erythromycin - pharmacology</subject><subject>Humans</subject><subject>Lipase - drug effects</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Pharmacology and Treatment</subject><subject>Pharmacology. Drug treatments</subject><subject>Propionibacterium acnes</subject><subject>Propionibacterium acnes - drug effects</subject><subject>Propionibacterium acnes - enzymology</subject><subject>Propionibacterium acnes - isolation & purification</subject><subject>Skin involvement in other diseases. Miscellaneous. 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Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Clindamycin - pharmacology</topic><topic>Dermatology</topic><topic>Drug Resistance, Bacterial</topic><topic>Erythromycin - pharmacology</topic><topic>Humans</topic><topic>Lipase - drug effects</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Pharmacology and Treatment</topic><topic>Pharmacology. Drug treatments</topic><topic>Propionibacterium acnes</topic><topic>Propionibacterium acnes - drug effects</topic><topic>Propionibacterium acnes - enzymology</topic><topic>Propionibacterium acnes - isolation & purification</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gloor, Max</creatorcontrib><creatorcontrib>Wasik, Barbara</creatorcontrib><creatorcontrib>Becker, Andrea</creatorcontrib><creatorcontrib>Höffler, Ulrich</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>MEDLINE - Academic</collection><jtitle>Dermatology (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gloor, Max</au><au>Wasik, Barbara</au><au>Becker, Andrea</au><au>Höffler, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Lipase Activity in Antibiotic-Resistant Propionibacterium acnes Strains</atitle><jtitle>Dermatology (Basel)</jtitle><addtitle>Dermatology</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>205</volume><issue>3</issue><spage>260</spage><epage>264</epage><pages>260-264</pages><issn>1018-8665</issn><eissn>1421-9832</eissn><abstract>Background and Objective: Erythromycin-sensitive and/or clindamycin-sensitive strains of Propionibacterium acnes show a reduced lipase production at levels below the minimal growth-inhibitory concentration (MIC). The objective of this study was to determine whether erythromycin and clindamycin concentrations far below the MIC inhibit lipase production in P. acnes strains resistant to these antibiotics. Methods: Of 42 P. acnes strains, 10 showed an MIC >256 µg/ml for erythromycin. Two strains showed MICs of 0.19 and 0.25 µg/ml, while the MIC of the remaining strains was ≤0.016 µg/ml. Lipase activity was determined up to a concentration of 192 µg/ml by cultivation on spirit blue agar + lipase reagent. The 10 strains whose erythromycin MIC was >256 µg/ml were also tested for lipase inhibition by clindamycin. While this method fails to differentiate between inhibition of lipase production and inhibition of lipase activity, the absence of inhibition of lipase activity rules out inhibition of lipase production. Results: Inhibition of lipolysis by sub-MIC concentrations was demonstrated only for clindamycin in 3 P. acnes strains. However, lipase inhibition was seen only at the dilution level immediately below the MIC. Conclusions: Resistant P. acnes strains with high erythromycin and/or clindamycin MICs can be ruled out to show in vitro inhibition of lipase production at antibiotic concentrations far below the MIC.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>12399674</pmid><doi>10.1159/000065856</doi><tpages>5</tpages></addata></record> |
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| subjects | Acne Vulgaris - microbiology Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Clindamycin - pharmacology Dermatology Drug Resistance, Bacterial Erythromycin - pharmacology Humans Lipase - drug effects Medical sciences Microbial Sensitivity Tests Pharmacology and Treatment Pharmacology. Drug treatments Propionibacterium acnes Propionibacterium acnes - drug effects Propionibacterium acnes - enzymology Propionibacterium acnes - isolation & purification Skin involvement in other diseases. Miscellaneous. General aspects |
| title | Inhibition of Lipase Activity in Antibiotic-Resistant Propionibacterium acnes Strains |
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