VLA-2 and VLA-5 Cell Adhesion Molecules Expression in CD34+ Cells from Umbilical Cord Blood and from Bone Marrow
Background/Aims: Umbilical cord blood contains a large number of early hematopoietic cells with high proliferating capacity, that has been used as an alternative to bone marrow transplantation. The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bon...
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| Veröffentlicht in: | Blood purification 2002-01, Vol.20 (2), p.174-176 |
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| container_title | Blood purification |
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| creator | Pafumi, C. Mancari, R. Parisi, G. Farina, M. Russo, A. Pernicone, G. Bandiera, S. Giardina, P. Mirisola, T. Stagno, F. Calogero, A.E. |
| description | Background/Aims: Umbilical cord blood contains a large number of early hematopoietic cells with high proliferating capacity, that has been used as an alternative to bone marrow transplantation. The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bone marrow. Methods: We investigated two integrins, named VLA-2 and VLA-5 (Very Late Appearing Antigen), expressed in the surface of CD34+ cells. The CD34+ cells, isolated with MACS CD34+ isolation kit, were labelled with the appropriate monoclonal antibodies. Results: Cell adhesion molecules showed highly expressed in both cord blood and bone marrow CD34+ cells. Conclusion: There are no significant differences between the two sources of CD34+ populations. |
| doi_str_mv | 10.1159/000047005 |
| format | Article |
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The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bone marrow. Methods: We investigated two integrins, named VLA-2 and VLA-5 (Very Late Appearing Antigen), expressed in the surface of CD34+ cells. The CD34+ cells, isolated with MACS CD34+ isolation kit, were labelled with the appropriate monoclonal antibodies. Results: Cell adhesion molecules showed highly expressed in both cord blood and bone marrow CD34+ cells. Conclusion: There are no significant differences between the two sources of CD34+ populations.</description><identifier>ISSN: 0253-5068</identifier><identifier>EISSN: 1421-9735</identifier><identifier>DOI: 10.1159/000047005</identifier><identifier>PMID: 11818681</identifier><identifier>CODEN: BLPUDO</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Antigens, CD34 - analysis ; Biological and medical sciences ; Bone Marrow Cells - chemistry ; Bone Marrow Cells - immunology ; Cell Adhesion Molecules - analysis ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cell Separation ; Fetal Blood - chemistry ; Fetal Blood - cytology ; Fetal Blood - immunology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoassay ; Integrin alpha2beta1 - analysis ; Integrin alpha5beta1 - analysis ; Molecular and cellular biology ; Original Paper</subject><ispartof>Blood purification, 2002-01, Vol.20 (2), p.174-176</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c320t-b1979a2c00e910cc7d248bd38129abd12c5a50354da99b6a9bfdb67e3023cd2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2427,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13484770$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11818681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pafumi, C.</creatorcontrib><creatorcontrib>Mancari, R.</creatorcontrib><creatorcontrib>Parisi, G.</creatorcontrib><creatorcontrib>Farina, M.</creatorcontrib><creatorcontrib>Russo, A.</creatorcontrib><creatorcontrib>Pernicone, G.</creatorcontrib><creatorcontrib>Bandiera, S.</creatorcontrib><creatorcontrib>Giardina, P.</creatorcontrib><creatorcontrib>Mirisola, T.</creatorcontrib><creatorcontrib>Stagno, F.</creatorcontrib><creatorcontrib>Calogero, A.E.</creatorcontrib><title>VLA-2 and VLA-5 Cell Adhesion Molecules Expression in CD34+ Cells from Umbilical Cord Blood and from Bone Marrow</title><title>Blood purification</title><addtitle>Blood Purif</addtitle><description>Background/Aims: Umbilical cord blood contains a large number of early hematopoietic cells with high proliferating capacity, that has been used as an alternative to bone marrow transplantation. The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bone marrow. Methods: We investigated two integrins, named VLA-2 and VLA-5 (Very Late Appearing Antigen), expressed in the surface of CD34+ cells. The CD34+ cells, isolated with MACS CD34+ isolation kit, were labelled with the appropriate monoclonal antibodies. Results: Cell adhesion molecules showed highly expressed in both cord blood and bone marrow CD34+ cells. Conclusion: There are no significant differences between the two sources of CD34+ populations.</description><subject>Antigens, CD34 - analysis</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells - chemistry</subject><subject>Bone Marrow Cells - immunology</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cell Separation</subject><subject>Fetal Blood - chemistry</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Blood - immunology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Integrin alpha2beta1 - analysis</subject><subject>Integrin alpha5beta1 - analysis</subject><subject>Molecular and cellular biology</subject><subject>Original Paper</subject><issn>0253-5068</issn><issn>1421-9735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M1P2zAYBnALDUFhHDhPQhYSSBPKeP0Vx8c2MIZUNA6Ua-TYDoQlcbBbDf77hbSDC77Y8vvTY-tB6JDAD0KEOodhcQkgttCEcEoSJZn4giZABUsEpNku2ovxCYDwVKgdtEtIRrI0IxPU38-nCcW6s_jtJHDumgZP7aOLte_wjW-cWTUu4suXPrg4XtYdzi8YPxttxFXwLV60Zd3URjc498HiWeO9HVPH6cx3Dt_oEPzfr2i70k10B5t9Hy1-Xt7lv5L576vrfDpPDKOwTEqipNLUADhFwBhpKc9KyzJClS4toUZoAUxwq5UqU63KypapdAwoM5Y6to9O17l98M8rF5dFW0czfFh3zq9iIQkXQCUd4Pc1NMHHGFxV9KFudXgtCBRv9Rbv9Q72aBO6KltnP-SmzwGcbICOQxlV0J2p44djPONSwuC-rd0fHR5ceAf_nzn-dDq7XYyg6G3F_gHdBZNr</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Pafumi, C.</creator><creator>Mancari, R.</creator><creator>Parisi, G.</creator><creator>Farina, M.</creator><creator>Russo, A.</creator><creator>Pernicone, G.</creator><creator>Bandiera, S.</creator><creator>Giardina, P.</creator><creator>Mirisola, T.</creator><creator>Stagno, F.</creator><creator>Calogero, A.E.</creator><general>Karger</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020101</creationdate><title>VLA-2 and VLA-5 Cell Adhesion Molecules Expression in CD34+ Cells from Umbilical Cord Blood and from Bone Marrow</title><author>Pafumi, C. ; Mancari, R. ; Parisi, G. ; Farina, M. ; Russo, A. ; Pernicone, G. ; Bandiera, S. ; Giardina, P. ; Mirisola, T. ; Stagno, F. ; Calogero, A.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-b1979a2c00e910cc7d248bd38129abd12c5a50354da99b6a9bfdb67e3023cd2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Antigens, CD34 - analysis</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells - chemistry</topic><topic>Bone Marrow Cells - immunology</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cell Separation</topic><topic>Fetal Blood - chemistry</topic><topic>Fetal Blood - cytology</topic><topic>Fetal Blood - immunology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Integrin alpha2beta1 - analysis</topic><topic>Integrin alpha5beta1 - analysis</topic><topic>Molecular and cellular biology</topic><topic>Original Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pafumi, C.</creatorcontrib><creatorcontrib>Mancari, R.</creatorcontrib><creatorcontrib>Parisi, G.</creatorcontrib><creatorcontrib>Farina, M.</creatorcontrib><creatorcontrib>Russo, A.</creatorcontrib><creatorcontrib>Pernicone, G.</creatorcontrib><creatorcontrib>Bandiera, S.</creatorcontrib><creatorcontrib>Giardina, P.</creatorcontrib><creatorcontrib>Mirisola, T.</creatorcontrib><creatorcontrib>Stagno, F.</creatorcontrib><creatorcontrib>Calogero, A.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood purification</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pafumi, C.</au><au>Mancari, R.</au><au>Parisi, G.</au><au>Farina, M.</au><au>Russo, A.</au><au>Pernicone, G.</au><au>Bandiera, S.</au><au>Giardina, P.</au><au>Mirisola, T.</au><au>Stagno, F.</au><au>Calogero, A.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VLA-2 and VLA-5 Cell Adhesion Molecules Expression in CD34+ Cells from Umbilical Cord Blood and from Bone Marrow</atitle><jtitle>Blood purification</jtitle><addtitle>Blood Purif</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>20</volume><issue>2</issue><spage>174</spage><epage>176</epage><pages>174-176</pages><issn>0253-5068</issn><eissn>1421-9735</eissn><coden>BLPUDO</coden><abstract>Background/Aims: Umbilical cord blood contains a large number of early hematopoietic cells with high proliferating capacity, that has been used as an alternative to bone marrow transplantation. The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bone marrow. Methods: We investigated two integrins, named VLA-2 and VLA-5 (Very Late Appearing Antigen), expressed in the surface of CD34+ cells. The CD34+ cells, isolated with MACS CD34+ isolation kit, were labelled with the appropriate monoclonal antibodies. Results: Cell adhesion molecules showed highly expressed in both cord blood and bone marrow CD34+ cells. Conclusion: There are no significant differences between the two sources of CD34+ populations.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>11818681</pmid><doi>10.1159/000047005</doi><tpages>3</tpages></addata></record> |
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| subjects | Antigens, CD34 - analysis Biological and medical sciences Bone Marrow Cells - chemistry Bone Marrow Cells - immunology Cell Adhesion Molecules - analysis Cell differentiation, maturation, development, hematopoiesis Cell physiology Cell Separation Fetal Blood - chemistry Fetal Blood - cytology Fetal Blood - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Immunoassay Integrin alpha2beta1 - analysis Integrin alpha5beta1 - analysis Molecular and cellular biology Original Paper |
| title | VLA-2 and VLA-5 Cell Adhesion Molecules Expression in CD34+ Cells from Umbilical Cord Blood and from Bone Marrow |
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