Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro
Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fl...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2016-01, Vol.39 (2), p.596-603 |
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| container_title | Cellular physiology and biochemistry |
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| creator | Ma, Haili Shi, Zeyu Dong, Yaoze Liang, Rui Chen, Jianshan Wang, Jinming Wang, Jundong |
| description | Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals. |
| doi_str_mv | 10.1159/000445651 |
| format | Article |
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Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000445651</identifier><identifier>PMID: 27415412</identifier><language>eng</language><publisher>Basel, Switzerland: Cell Physiol Biochem Press GmbH & Co KG</publisher><subject>Animals ; B cell ; B-Lymphocytes - drug effects ; B-Lymphocytes - metabolism ; B7-1 Antigen - metabolism ; B7-2 Antigen - metabolism ; Biomarkers - metabolism ; CD40 Antigens - metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endocytosis ; Endocytosis - drug effects ; Female ; Flow Cytometry ; Fluoride ; Fluorides - pharmacology ; Lipopolysaccharides - pharmacology ; Membrane Proteins - metabolism ; Mice ; Original Paper ; Sodium Fluoride - pharmacology ; Spleen - cytology ; Syndecan-1 - metabolism</subject><ispartof>Cellular physiology and biochemistry, 2016-01, Vol.39 (2), p.596-603</ispartof><rights>2016 The Author(s) Published by S. Karger AG, Basel</rights><rights>2016 The Author(s) Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-2ee344e09e6f3a550ecef314420fa01bfabada6336cc0a5771d6b7895bdd45f83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,2096,27612,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27415412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Haili</creatorcontrib><creatorcontrib>Shi, Zeyu</creatorcontrib><creatorcontrib>Dong, Yaoze</creatorcontrib><creatorcontrib>Liang, Rui</creatorcontrib><creatorcontrib>Chen, Jianshan</creatorcontrib><creatorcontrib>Wang, Jinming</creatorcontrib><creatorcontrib>Wang, Jundong</creatorcontrib><title>Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.</description><subject>Animals</subject><subject>B cell</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - metabolism</subject><subject>B7-1 Antigen - metabolism</subject><subject>B7-2 Antigen - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>CD40 Antigens - metabolism</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluoride</subject><subject>Fluorides - pharmacology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Original Paper</subject><subject>Sodium Fluoride - pharmacology</subject><subject>Spleen - cytology</subject><subject>Syndecan-1 - metabolism</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNptkU1v1DAQhi1ERUvLgTtClrjQQ6gd27F9pKstXWkrkPi4GsceV2mz8WInFf33eJvtnjjNePT4GY1ehN5S8olSoS8IIZyLRtAX6ITymlZaSvWy9ISKSmklj9HrnO9IeUpdv0LHteRUcFqfoN_LEMCNOAZ81U8xdR5wHPBy8NE9jjF3GdvB4-9TCtYBvrHpHhJe_t0myLkr5BoeoM-7_zdxyoAv8QL6MlgN-Fc3pniGjoLtM7zZ11P082r5Y3Fdrb9-WS0-ryvHmRirGoBxDkRDE5gVgoCDwCjnNQmW0DbY1nrbMNY4R6yQkvqmlUqL1nsugmKnaDV7fbR3Zpu6jU2PJtrOPA1iujU2jZ3rwUitg2-90Jy2vJZCKU2o9kzuqgNdXB9n1zbFPxPk0Wy67MpZdoBypKGKKKYayeuCns-oSzHnBOGwmhKzC8ccwins-712ajfgD-RzGgX4MAP3Nt1COgCLb5ezwmx9KNS7_1L7Lf8AC0Ocuw</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Ma, Haili</creator><creator>Shi, Zeyu</creator><creator>Dong, Yaoze</creator><creator>Liang, Rui</creator><creator>Chen, Jianshan</creator><creator>Wang, Jinming</creator><creator>Wang, Jundong</creator><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20160101</creationdate><title>Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro</title><author>Ma, Haili ; Shi, Zeyu ; Dong, Yaoze ; Liang, Rui ; Chen, Jianshan ; Wang, Jinming ; Wang, Jundong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-2ee344e09e6f3a550ecef314420fa01bfabada6336cc0a5771d6b7895bdd45f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>B cell</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - metabolism</topic><topic>B7-1 Antigen - metabolism</topic><topic>B7-2 Antigen - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>CD40 Antigens - metabolism</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluoride</topic><topic>Fluorides - pharmacology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Original Paper</topic><topic>Sodium Fluoride - pharmacology</topic><topic>Spleen - cytology</topic><topic>Syndecan-1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Haili</creatorcontrib><creatorcontrib>Shi, Zeyu</creatorcontrib><creatorcontrib>Dong, Yaoze</creatorcontrib><creatorcontrib>Liang, Rui</creatorcontrib><creatorcontrib>Chen, Jianshan</creatorcontrib><creatorcontrib>Wang, Jinming</creatorcontrib><creatorcontrib>Wang, Jundong</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Haili</au><au>Shi, Zeyu</au><au>Dong, Yaoze</au><au>Liang, Rui</au><au>Chen, Jianshan</au><au>Wang, Jinming</au><au>Wang, Jundong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>39</volume><issue>2</issue><spage>596</spage><epage>603</epage><pages>596-603</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.</abstract><cop>Basel, Switzerland</cop><pub>Cell Physiol Biochem Press GmbH & Co KG</pub><pmid>27415412</pmid><doi>10.1159/000445651</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals B cell B-Lymphocytes - drug effects B-Lymphocytes - metabolism B7-1 Antigen - metabolism B7-2 Antigen - metabolism Biomarkers - metabolism CD40 Antigens - metabolism Cells, Cultured Dose-Response Relationship, Drug Endocytosis Endocytosis - drug effects Female Flow Cytometry Fluoride Fluorides - pharmacology Lipopolysaccharides - pharmacology Membrane Proteins - metabolism Mice Original Paper Sodium Fluoride - pharmacology Spleen - cytology Syndecan-1 - metabolism |
| title | Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro |
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