Upregulation of Long Non-Coding RNA PlncRNA-1 Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma
Background/Aims: PlncRNA-1 has been demonstrated to promote malignancy in various cancers. The present study aims to investigate the expression pattern, prognosis value and the function of PlncRNA-1 in human hepatocellular carcinoma (HCC). Methods: The expression of PlncRNA-1 in 84 pairs of HCC and...
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| Veröffentlicht in: | Cellular Physiology and Biochemistry 2016-01, Vol.38 (2), p.836-846 |
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| creator | Dong, Liyang Ni, Junwei Hu, Wenhao Yu, Chang Li, Haiyan |
| description | Background/Aims: PlncRNA-1 has been demonstrated to promote malignancy in various cancers. The present study aims to investigate the expression pattern, prognosis value and the function of PlncRNA-1 in human hepatocellular carcinoma (HCC). Methods: The expression of PlncRNA-1 in 84 pairs of HCC and their matched normal tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of PlncRNA-1 expression and clinicopathological characteristics and prognosis were also analyzed. The biological role of PlncRNA-1 in cell proliferation, migration and invasion was examined in vitro and in vivo. Results: The results showed that the level of PlncRNA-1 expression was significantly increased in HCC tissues and significantly correlated with tumor size, vascular invasion and advanced TNM stage. Moreover, patients with high levels of PlncRNA-1 expression had relatively poor prognostic outcomes, serving as an independent prognostic factor for HCC. In vitro functional assays indicated that knockdown of PlncRNA-1 expression significantly reduced cell proliferation, migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT) signaling. Animal model experiments confirmed the ability of PlncRNA-1 to promote tumor growth in vivo. Conclusions: Taken together, our findings suggest that PlncRNA-1 may serve as an oncogene in HCC progression and represent a valuable prognostic marker and potential therapeutic target for HCC. |
| doi_str_mv | 10.1159/000443038 |
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The present study aims to investigate the expression pattern, prognosis value and the function of PlncRNA-1 in human hepatocellular carcinoma (HCC). Methods: The expression of PlncRNA-1 in 84 pairs of HCC and their matched normal tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of PlncRNA-1 expression and clinicopathological characteristics and prognosis were also analyzed. The biological role of PlncRNA-1 in cell proliferation, migration and invasion was examined in vitro and in vivo. Results: The results showed that the level of PlncRNA-1 expression was significantly increased in HCC tissues and significantly correlated with tumor size, vascular invasion and advanced TNM stage. Moreover, patients with high levels of PlncRNA-1 expression had relatively poor prognostic outcomes, serving as an independent prognostic factor for HCC. In vitro functional assays indicated that knockdown of PlncRNA-1 expression significantly reduced cell proliferation, migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT) signaling. Animal model experiments confirmed the ability of PlncRNA-1 to promote tumor growth in vivo. Conclusions: Taken together, our findings suggest that PlncRNA-1 may serve as an oncogene in HCC progression and represent a valuable prognostic marker and potential therapeutic target for HCC.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000443038</identifier><identifier>PMID: 26906068</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Carcinoma, Hepatocellular - diagnosis ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cell Movement ; Development and progression ; Epithelial-Mesenchymal Transition ; Female ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Genetic regulation ; Health aspects ; Hepatocellular carcinoma ; Hepatoma ; Humans ; Invasion ; Liver - pathology ; Liver Neoplasms - diagnosis ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Male ; Mice, Inbred BALB C ; Middle Aged ; Neoplasm Invasiveness - genetics ; Neoplasm Invasiveness - pathology ; Neoplasm Metastasis - genetics ; Neoplasm Metastasis - pathology ; Oncogene ; Original Paper ; PlncRNA-1 ; Prognosis ; Proliferation ; RNA ; RNA, Long Noncoding - genetics ; Up-Regulation</subject><ispartof>Cellular Physiology and Biochemistry, 2016-01, Vol.38 (2), p.836-846</ispartof><rights>2016 The Author(s) Published by S. Karger AG, Basel</rights><rights>2016 The Author(s) Published by S. Karger AG, Basel.</rights><rights>COPYRIGHT 2016 S. Karger AG</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-ce2d96f1f74dd0767eb43c22fa96372716f4a51069c3139990dd6e329ab599253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,865,2103,27640,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26906068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Liyang</creatorcontrib><creatorcontrib>Ni, Junwei</creatorcontrib><creatorcontrib>Hu, Wenhao</creatorcontrib><creatorcontrib>Yu, Chang</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><title>Upregulation of Long Non-Coding RNA PlncRNA-1 Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma</title><title>Cellular Physiology and Biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: PlncRNA-1 has been demonstrated to promote malignancy in various cancers. The present study aims to investigate the expression pattern, prognosis value and the function of PlncRNA-1 in human hepatocellular carcinoma (HCC). Methods: The expression of PlncRNA-1 in 84 pairs of HCC and their matched normal tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of PlncRNA-1 expression and clinicopathological characteristics and prognosis were also analyzed. The biological role of PlncRNA-1 in cell proliferation, migration and invasion was examined in vitro and in vivo. Results: The results showed that the level of PlncRNA-1 expression was significantly increased in HCC tissues and significantly correlated with tumor size, vascular invasion and advanced TNM stage. Moreover, patients with high levels of PlncRNA-1 expression had relatively poor prognostic outcomes, serving as an independent prognostic factor for HCC. In vitro functional assays indicated that knockdown of PlncRNA-1 expression significantly reduced cell proliferation, migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT) signaling. Animal model experiments confirmed the ability of PlncRNA-1 to promote tumor growth in vivo. Conclusions: Taken together, our findings suggest that PlncRNA-1 may serve as an oncogene in HCC progression and represent a valuable prognostic marker and potential therapeutic target for HCC.</description><subject>Animals</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Development and progression</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Health aspects</subject><subject>Hepatocellular carcinoma</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Invasion</subject><subject>Liver - pathology</subject><subject>Liver Neoplasms - diagnosis</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>Mice, Inbred BALB C</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Invasiveness - pathology</subject><subject>Neoplasm Metastasis - genetics</subject><subject>Neoplasm Metastasis - pathology</subject><subject>Oncogene</subject><subject>Original Paper</subject><subject>PlncRNA-1</subject><subject>Prognosis</subject><subject>Proliferation</subject><subject>RNA</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Up-Regulation</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNptkU1v1DAQhiMEomXhwB2hSFzgkGI7cRwfl6jQlbZlhdpzNGuPty6JndrZQ38Dfxpvs-wJ2dJ86Jl3ZjRZ9p6SC0q5_EoIqaqSlM2L7JxWjBZSiOZl8gnlRSMbcZa9ifGBpFBI9jo7Y7UkNamb8-zP3Rhwt-9hst7l3uRr73b5jXdF67VN7q-bZb7pnUq2oPkm-MFPGPNrnCCmb2MOTucrp_cqpS9HO91jb6EvrjGiU_dPA_T5bQAX7XML6_IrHGHyCvs-9Q15C0FZ5wd4m70y0Ed8d7SL7O775W17Vax__li1y3WhOGFToZBpWRtqRKU1EbXAbVUqxgzIuhRM0NpUwCmppSppKaUkWtdYMglbLiXj5SJbzbraw0M3BjtAeOo82O454cOugzBZ1WOnNJQNIcA58gq1lpJXXDSNJAINq2jS-jxrjcE_7jFO3WDjYTVw6Pexo6JueM2b8oBezOgOkrJ1xk8BVHoaB6u8Q2NTfpl24KSsUski-zIXqOBjDGhOs1LSHQ7fnQ6f2I_HOfbbAfWJ_HfpBHyagd8QdhhOQLv5Nkt0ozaJ-vBf6tjlL_GMvA0</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Dong, Liyang</creator><creator>Ni, Junwei</creator><creator>Hu, Wenhao</creator><creator>Yu, Chang</creator><creator>Li, Haiyan</creator><general>S. Karger AG</general><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20160101</creationdate><title>Upregulation of Long Non-Coding RNA PlncRNA-1 Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma</title><author>Dong, Liyang ; Ni, Junwei ; Hu, Wenhao ; Yu, Chang ; Li, Haiyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-ce2d96f1f74dd0767eb43c22fa96372716f4a51069c3139990dd6e329ab599253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Carcinoma, Hepatocellular - diagnosis</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Development and progression</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Health aspects</topic><topic>Hepatocellular carcinoma</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Invasion</topic><topic>Liver - pathology</topic><topic>Liver Neoplasms - diagnosis</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>Mice, Inbred BALB C</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Neoplasm Metastasis - genetics</topic><topic>Neoplasm Metastasis - pathology</topic><topic>Oncogene</topic><topic>Original Paper</topic><topic>PlncRNA-1</topic><topic>Prognosis</topic><topic>Proliferation</topic><topic>RNA</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Liyang</creatorcontrib><creatorcontrib>Ni, Junwei</creatorcontrib><creatorcontrib>Hu, Wenhao</creatorcontrib><creatorcontrib>Yu, Chang</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular Physiology and Biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Liyang</au><au>Ni, Junwei</au><au>Hu, Wenhao</au><au>Yu, Chang</au><au>Li, Haiyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of Long Non-Coding RNA PlncRNA-1 Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma</atitle><jtitle>Cellular Physiology and Biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>38</volume><issue>2</issue><spage>836</spage><epage>846</epage><pages>836-846</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: PlncRNA-1 has been demonstrated to promote malignancy in various cancers. The present study aims to investigate the expression pattern, prognosis value and the function of PlncRNA-1 in human hepatocellular carcinoma (HCC). Methods: The expression of PlncRNA-1 in 84 pairs of HCC and their matched normal tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of PlncRNA-1 expression and clinicopathological characteristics and prognosis were also analyzed. The biological role of PlncRNA-1 in cell proliferation, migration and invasion was examined in vitro and in vivo. Results: The results showed that the level of PlncRNA-1 expression was significantly increased in HCC tissues and significantly correlated with tumor size, vascular invasion and advanced TNM stage. Moreover, patients with high levels of PlncRNA-1 expression had relatively poor prognostic outcomes, serving as an independent prognostic factor for HCC. In vitro functional assays indicated that knockdown of PlncRNA-1 expression significantly reduced cell proliferation, migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT) signaling. Animal model experiments confirmed the ability of PlncRNA-1 to promote tumor growth in vivo. Conclusions: Taken together, our findings suggest that PlncRNA-1 may serve as an oncogene in HCC progression and represent a valuable prognostic marker and potential therapeutic target for HCC.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>26906068</pmid><doi>10.1159/000443038</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cellular Physiology and Biochemistry, 2016-01, Vol.38 (2), p.836-846 |
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| subjects | Animals Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell Movement Development and progression Epithelial-Mesenchymal Transition Female Gene Expression Regulation, Neoplastic Genetic aspects Genetic regulation Health aspects Hepatocellular carcinoma Hepatoma Humans Invasion Liver - pathology Liver Neoplasms - diagnosis Liver Neoplasms - genetics Liver Neoplasms - pathology Male Mice, Inbred BALB C Middle Aged Neoplasm Invasiveness - genetics Neoplasm Invasiveness - pathology Neoplasm Metastasis - genetics Neoplasm Metastasis - pathology Oncogene Original Paper PlncRNA-1 Prognosis Proliferation RNA RNA, Long Noncoding - genetics Up-Regulation |
| title | Upregulation of Long Non-Coding RNA PlncRNA-1 Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma |
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