Evaluation of p40 as a Myoepithelial Marker in Different Breast Lesions
Objective: The identification of myoepithelial cells (MEC) is a valuable clue in the differential diagnosis of breast lesions. A series of breast lesions with occasional absence of or decrease in the staining for some MEC markers was analyzed for the expression of a novel marker, p40, and results we...
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| Veröffentlicht in: | Pathobiology (Basel) 2015-09, Vol.82 (3-4), p.166-171 |
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| creator | Kővári, Bence Szász, A. Marcell Kulka, Janina Marušić, Zlatko Šarčević, Bozena Tiszlavicz, László Cserni, Gábor |
| description | Objective: The identification of myoepithelial cells (MEC) is a valuable clue in the differential diagnosis of breast lesions. A series of breast lesions with occasional absence of or decrease in the staining for some MEC markers was analyzed for the expression of a novel marker, p40, and results were compared to the p63 staining profile. Methods: Samples (n = 34) from patients with benign sclerosing lesions (n = 11), ductal carcinoma in situ (n = 13) and adenomyoepithelial lesions (n = 10) and associated normal breast tissues (n = 31) were selected to evaluate the differential expression of p40 and p63 using immunohistochemistry. Triple-negative, cytokeratin 5 (CK5)-expressing invasive breast carcinomas (n = 19) were also assessed for p40 expression. Results: Normal structures showed similar diffuse and strong MEC positivity using p40 and p63 in all 31 cases. The two antibodies performed similarly in all 34 breast lesions acknowledged to present altered expression of MEC markers; focal losses of expression occurred in a parallel fashion. CK5-positive carcinomas expressed p40 more frequently than p63 (18/19 vs. 8/19) and the staining was more marked. Conclusions: It seems that both antibodies can be used interchangeably for MEC identification, but show differences in the labeling at least in a subset of tumor cells in triple-negative carcinomas. |
| doi_str_mv | 10.1159/000375127 |
| format | Article |
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Marcell ; Kulka, Janina ; Marušić, Zlatko ; Šarčević, Bozena ; Tiszlavicz, László ; Cserni, Gábor</creator><creatorcontrib>Kővári, Bence ; Szász, A. Marcell ; Kulka, Janina ; Marušić, Zlatko ; Šarčević, Bozena ; Tiszlavicz, László ; Cserni, Gábor</creatorcontrib><description>Objective: The identification of myoepithelial cells (MEC) is a valuable clue in the differential diagnosis of breast lesions. A series of breast lesions with occasional absence of or decrease in the staining for some MEC markers was analyzed for the expression of a novel marker, p40, and results were compared to the p63 staining profile. Methods: Samples (n = 34) from patients with benign sclerosing lesions (n = 11), ductal carcinoma in situ (n = 13) and adenomyoepithelial lesions (n = 10) and associated normal breast tissues (n = 31) were selected to evaluate the differential expression of p40 and p63 using immunohistochemistry. Triple-negative, cytokeratin 5 (CK5)-expressing invasive breast carcinomas (n = 19) were also assessed for p40 expression. Results: Normal structures showed similar diffuse and strong MEC positivity using p40 and p63 in all 31 cases. The two antibodies performed similarly in all 34 breast lesions acknowledged to present altered expression of MEC markers; focal losses of expression occurred in a parallel fashion. CK5-positive carcinomas expressed p40 more frequently than p63 (18/19 vs. 8/19) and the staining was more marked. Conclusions: It seems that both antibodies can be used interchangeably for MEC identification, but show differences in the labeling at least in a subset of tumor cells in triple-negative carcinomas.</description><identifier>ISSN: 1015-2008</identifier><identifier>ISBN: 9783318055870</identifier><identifier>ISBN: 3318055875</identifier><identifier>EISSN: 1423-0291</identifier><identifier>EISBN: 9783318055887</identifier><identifier>EISBN: 3318055883</identifier><identifier>DOI: 10.1159/000375127</identifier><identifier>PMID: 26330357</identifier><identifier>CODEN: PATHEF</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Adenomyoepithelioma - metabolism ; Adult ; Aged ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Breast - metabolism ; Breast cancer ; Breast Diseases - metabolism ; Breast Neoplasms - diagnosis ; Breast Neoplasms - metabolism ; Carcinoma, Intraductal, Noninfiltrating - metabolism ; Diagnosis, Differential ; Epithelial Cells - metabolism ; Female ; Humans ; Immunohistochemistry ; Lesions ; Medical diagnosis ; Middle Aged ; Original Paper ; Transcription Factors - metabolism ; Triple Negative Breast Neoplasms - genetics ; Triple Negative Breast Neoplasms - metabolism ; Tumor Suppressor Proteins - metabolism ; Tumors</subject><ispartof>Pathobiology (Basel), 2015-09, Vol.82 (3-4), p.166-171</ispartof><rights>2015 S. Karger AG, Basel</rights><rights>2015 S. Karger AG, Basel.</rights><rights>Copyright S. Karger AG Sep 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-763cee99023445e8b1d365d717f7489235711a49a66e7a1fccf76911aeb232a3</citedby><cites>FETCH-LOGICAL-c334t-763cee99023445e8b1d365d717f7489235711a49a66e7a1fccf76911aeb232a3</cites><orcidid>0000-0002-2733-0822</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2430,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26330357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kővári, Bence</creatorcontrib><creatorcontrib>Szász, A. 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Methods: Samples (n = 34) from patients with benign sclerosing lesions (n = 11), ductal carcinoma in situ (n = 13) and adenomyoepithelial lesions (n = 10) and associated normal breast tissues (n = 31) were selected to evaluate the differential expression of p40 and p63 using immunohistochemistry. Triple-negative, cytokeratin 5 (CK5)-expressing invasive breast carcinomas (n = 19) were also assessed for p40 expression. Results: Normal structures showed similar diffuse and strong MEC positivity using p40 and p63 in all 31 cases. The two antibodies performed similarly in all 34 breast lesions acknowledged to present altered expression of MEC markers; focal losses of expression occurred in a parallel fashion. CK5-positive carcinomas expressed p40 more frequently than p63 (18/19 vs. 8/19) and the staining was more marked. 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Marcell</au><au>Kulka, Janina</au><au>Marušić, Zlatko</au><au>Šarčević, Bozena</au><au>Tiszlavicz, László</au><au>Cserni, Gábor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of p40 as a Myoepithelial Marker in Different Breast Lesions</atitle><jtitle>Pathobiology (Basel)</jtitle><addtitle>Pathobiology</addtitle><date>2015-09</date><risdate>2015</risdate><volume>82</volume><issue>3-4</issue><spage>166</spage><epage>171</epage><pages>166-171</pages><issn>1015-2008</issn><eissn>1423-0291</eissn><isbn>9783318055870</isbn><isbn>3318055875</isbn><eisbn>9783318055887</eisbn><eisbn>3318055883</eisbn><coden>PATHEF</coden><abstract>Objective: The identification of myoepithelial cells (MEC) is a valuable clue in the differential diagnosis of breast lesions. A series of breast lesions with occasional absence of or decrease in the staining for some MEC markers was analyzed for the expression of a novel marker, p40, and results were compared to the p63 staining profile. Methods: Samples (n = 34) from patients with benign sclerosing lesions (n = 11), ductal carcinoma in situ (n = 13) and adenomyoepithelial lesions (n = 10) and associated normal breast tissues (n = 31) were selected to evaluate the differential expression of p40 and p63 using immunohistochemistry. Triple-negative, cytokeratin 5 (CK5)-expressing invasive breast carcinomas (n = 19) were also assessed for p40 expression. Results: Normal structures showed similar diffuse and strong MEC positivity using p40 and p63 in all 31 cases. The two antibodies performed similarly in all 34 breast lesions acknowledged to present altered expression of MEC markers; focal losses of expression occurred in a parallel fashion. CK5-positive carcinomas expressed p40 more frequently than p63 (18/19 vs. 8/19) and the staining was more marked. Conclusions: It seems that both antibodies can be used interchangeably for MEC identification, but show differences in the labeling at least in a subset of tumor cells in triple-negative carcinomas.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>26330357</pmid><doi>10.1159/000375127</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-2733-0822</orcidid></addata></record> |
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| subjects | Adenomyoepithelioma - metabolism Adult Aged Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Breast - metabolism Breast cancer Breast Diseases - metabolism Breast Neoplasms - diagnosis Breast Neoplasms - metabolism Carcinoma, Intraductal, Noninfiltrating - metabolism Diagnosis, Differential Epithelial Cells - metabolism Female Humans Immunohistochemistry Lesions Medical diagnosis Middle Aged Original Paper Transcription Factors - metabolism Triple Negative Breast Neoplasms - genetics Triple Negative Breast Neoplasms - metabolism Tumor Suppressor Proteins - metabolism Tumors |
| title | Evaluation of p40 as a Myoepithelial Marker in Different Breast Lesions |
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