Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?
Aim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham...
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creator | Ergenoglu, Mete Erbaş, Oytun Akdemir, Ali Yeniel, Ahmet Özgür Yildirim, Nuri Oltulu, Fatih Aktuğ, Hüseyin Taskiran, Dilek |
description | Aim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model. |
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Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model.</description><identifier>ISSN: 0014-312X</identifier><identifier>EISSN: 1421-9921</identifier><identifier>DOI: 10.1159/000353403</identifier><identifier>PMID: 23899611</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Antipyrine - analogs & derivatives ; Antipyrine - therapeutic use ; Caspase 3 - analysis ; Female ; Free Radical Scavengers - therapeutic use ; Glutathione - analysis ; Immunohistochemistry ; Malondialdehyde - analysis ; Original Paper ; Ovary - blood supply ; Ovary - pathology ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control</subject><ispartof>European surgical research, 2013-01, Vol.51 (1-2), p.21-32</ispartof><rights>2013 S. Karger AG, Basel</rights><rights>2013 S. Karger AG, Basel.</rights><rights>Copyright (c) 2013 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-46d3efc3d591dab2ed33cfe181b31bcb3c4738806c90066065963bedef117fa63</citedby><cites>FETCH-LOGICAL-c362t-46d3efc3d591dab2ed33cfe181b31bcb3c4738806c90066065963bedef117fa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23899611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ergenoglu, Mete</creatorcontrib><creatorcontrib>Erbaş, Oytun</creatorcontrib><creatorcontrib>Akdemir, Ali</creatorcontrib><creatorcontrib>Yeniel, Ahmet Özgür</creatorcontrib><creatorcontrib>Yildirim, Nuri</creatorcontrib><creatorcontrib>Oltulu, Fatih</creatorcontrib><creatorcontrib>Aktuğ, Hüseyin</creatorcontrib><creatorcontrib>Taskiran, Dilek</creatorcontrib><title>Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?</title><title>European surgical research</title><addtitle>Eur Surg Res</addtitle><description>Aim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model.</description><subject>Animals</subject><subject>Antipyrine - analogs & derivatives</subject><subject>Antipyrine - therapeutic use</subject><subject>Caspase 3 - analysis</subject><subject>Female</subject><subject>Free Radical Scavengers - therapeutic use</subject><subject>Glutathione - analysis</subject><subject>Immunohistochemistry</subject><subject>Malondialdehyde - analysis</subject><subject>Original Paper</subject><subject>Ovary - blood supply</subject><subject>Ovary - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><issn>0014-312X</issn><issn>1421-9921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpt0MFP2zAUBnBrAo2u24H7NFnish0CfnmJE3OZEO2gElKnwiRukWM_s0ATd3aCxH9PUKGHiZNl-_c-PX2MHYI4BsjViRACc8wEfmATyFJIlEphj02EgCxBSG8P2KcY78drrgr1kR2kWColASbMnvU9dYPuG99x7_gimr_UNvpkRRsKbojje7Lo7GDI8uWjDo3u-Ey3-o540_GV7uMpn3mKfG510I--I750jgL_HXxP5iX352e27_Q60pfXc8r-_JrfnF8mV8uLxfnZVWJQpn2SSYvkDNpcgdV1ShbROIISaoTa1GiyAstSSKOEkFLIXEmsyZIDKJyWOGXft7mb4P8NFPuqbaKh9Vp35IdYQSZTLECUYqRH_9F7P4Ru3G5UBZQIeV6O6sdWmeBjDOSqTWhaHZ4qENVL9dWu-tF-e00c6pbsTr51PYKvW_Cgwx2FHdjNH737Pb9ebUW1sQ6fATfEkiA</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Ergenoglu, Mete</creator><creator>Erbaş, Oytun</creator><creator>Akdemir, Ali</creator><creator>Yeniel, Ahmet Özgür</creator><creator>Yildirim, Nuri</creator><creator>Oltulu, Fatih</creator><creator>Aktuğ, Hüseyin</creator><creator>Taskiran, Dilek</creator><general>S. 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analogs & derivatives</topic><topic>Antipyrine - therapeutic use</topic><topic>Caspase 3 - analysis</topic><topic>Female</topic><topic>Free Radical Scavengers - therapeutic use</topic><topic>Glutathione - analysis</topic><topic>Immunohistochemistry</topic><topic>Malondialdehyde - analysis</topic><topic>Original Paper</topic><topic>Ovary - blood supply</topic><topic>Ovary - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ergenoglu, Mete</creatorcontrib><creatorcontrib>Erbaş, Oytun</creatorcontrib><creatorcontrib>Akdemir, Ali</creatorcontrib><creatorcontrib>Yeniel, Ahmet Özgür</creatorcontrib><creatorcontrib>Yildirim, Nuri</creatorcontrib><creatorcontrib>Oltulu, Fatih</creatorcontrib><creatorcontrib>Aktuğ, Hüseyin</creatorcontrib><creatorcontrib>Taskiran, Dilek</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>European surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ergenoglu, Mete</au><au>Erbaş, Oytun</au><au>Akdemir, Ali</au><au>Yeniel, Ahmet Özgür</au><au>Yildirim, Nuri</au><au>Oltulu, Fatih</au><au>Aktuğ, Hüseyin</au><au>Taskiran, Dilek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?</atitle><jtitle>European surgical research</jtitle><addtitle>Eur Surg Res</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>51</volume><issue>1-2</issue><spage>21</spage><epage>32</epage><pages>21-32</pages><issn>0014-312X</issn><eissn>1421-9921</eissn><abstract>Aim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>23899611</pmid><doi>10.1159/000353403</doi><tpages>12</tpages></addata></record> |
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subjects | Animals Antipyrine - analogs & derivatives Antipyrine - therapeutic use Caspase 3 - analysis Female Free Radical Scavengers - therapeutic use Glutathione - analysis Immunohistochemistry Malondialdehyde - analysis Original Paper Ovary - blood supply Ovary - pathology Rats Rats, Sprague-Dawley Reperfusion Injury - metabolism Reperfusion Injury - pathology Reperfusion Injury - prevention & control |
title | Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection? |
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