Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes
Objective: To examine the possible relationship between maternal and fetal characteristics and pregnancy outcomes on fetal and maternal cell-free (cf) DNA in maternal plasma at 11-13 weeks' gestation. Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosom...
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| Veröffentlicht in: | Fetal diagnosis and therapy 2013-05, Vol.33 (4), p.215-223 |
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| creator | Poon, L.C.Y. Musci, T. Song, K. Syngelaki, A. Nicolaides, K.H. |
| description | Objective: To examine the possible relationship between maternal and fetal characteristics and pregnancy outcomes on fetal and maternal cell-free (cf) DNA in maternal plasma at 11-13 weeks' gestation. Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosome-selective sequencing was used to determine the proportion of cfDNA and total cfDNA counts which was of fetal and maternal origin. Multivariate regression analysis was used to determine whether specific maternal and fetal characteristics and pregnancy complications, such as preeclampsia (PE), early spontaneous preterm birth (SPB) and delivery of small for gestational age (SGA) neonates, were significant predictors of fetal and maternal cfDNA in maternal plasma. Results: The fetal and maternal cfDNA plasma concentration increased with serum pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin level, was higher in women of Afro-Caribbean and East-Asian racial origin than in Caucasians, and lower in smokers, but it was not significantly altered in pregnancies complicated by PE, SPB or SGA. The fetal cfDNA level was inversely related to maternal weight and uterine artery pulsatility index, and maternal cfDNA increased with maternal weight. Conclusions: The fetal and maternal cfDNA level in maternal plasma is affected by maternal and fetal characteristics, but it is not altered in pregnancy complications. |
| doi_str_mv | 10.1159/000346806 |
| format | Article |
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Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosome-selective sequencing was used to determine the proportion of cfDNA and total cfDNA counts which was of fetal and maternal origin. Multivariate regression analysis was used to determine whether specific maternal and fetal characteristics and pregnancy complications, such as preeclampsia (PE), early spontaneous preterm birth (SPB) and delivery of small for gestational age (SGA) neonates, were significant predictors of fetal and maternal cfDNA in maternal plasma. Results: The fetal and maternal cfDNA plasma concentration increased with serum pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin level, was higher in women of Afro-Caribbean and East-Asian racial origin than in Caucasians, and lower in smokers, but it was not significantly altered in pregnancies complicated by PE, SPB or SGA. The fetal cfDNA level was inversely related to maternal weight and uterine artery pulsatility index, and maternal cfDNA increased with maternal weight. Conclusions: The fetal and maternal cfDNA level in maternal plasma is affected by maternal and fetal characteristics, but it is not altered in pregnancy complications.</description><identifier>ISSN: 1015-3837</identifier><identifier>EISSN: 1421-9964</identifier><identifier>DOI: 10.1159/000346806</identifier><identifier>PMID: 23466432</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adult ; Biological and medical sciences ; Biomarkers - blood ; Cohort Studies ; Diseases of mother, fetus and pregnancy ; DNA - blood ; Early Diagnosis ; Female ; Fetal Growth Retardation - blood ; Fetal Growth Retardation - diagnosis ; Fetal Growth Retardation - physiopathology ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Original Paper ; Placentation ; Plasma - metabolism ; Pre-Eclampsia - blood ; Pre-Eclampsia - diagnosis ; Pre-Eclampsia - physiopathology ; Pregnancy ; Pregnancy Complications - blood ; Pregnancy Complications - diagnosis ; Pregnancy Complications - physiopathology ; Pregnancy Trimester, First ; Pregnancy. Fetus. Placenta ; Premature Birth - blood ; Premature Birth - diagnosis ; Premature Birth - physiopathology ; Prenatal Diagnosis ; Prospective Studies ; Pulsatile Flow ; Uterine Artery - physiology ; Uterine Artery - physiopathology ; Weight Loss</subject><ispartof>Fetal diagnosis and therapy, 2013-05, Vol.33 (4), p.215-223</ispartof><rights>2013 S. Karger AG, Basel</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 S. Karger AG, Basel.</rights><rights>Copyright (c) 2013 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-2f40b03533abc73bb7f3f2c7044f941b90ceed49e0e2d8901b28b98f8878444d3</citedby><cites>FETCH-LOGICAL-c465t-2f40b03533abc73bb7f3f2c7044f941b90ceed49e0e2d8901b28b98f8878444d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27406066$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23466432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poon, L.C.Y.</creatorcontrib><creatorcontrib>Musci, T.</creatorcontrib><creatorcontrib>Song, K.</creatorcontrib><creatorcontrib>Syngelaki, A.</creatorcontrib><creatorcontrib>Nicolaides, K.H.</creatorcontrib><title>Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes</title><title>Fetal diagnosis and therapy</title><addtitle>Fetal Diagn Ther</addtitle><description>Objective: To examine the possible relationship between maternal and fetal characteristics and pregnancy outcomes on fetal and maternal cell-free (cf) DNA in maternal plasma at 11-13 weeks' gestation. Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosome-selective sequencing was used to determine the proportion of cfDNA and total cfDNA counts which was of fetal and maternal origin. Multivariate regression analysis was used to determine whether specific maternal and fetal characteristics and pregnancy complications, such as preeclampsia (PE), early spontaneous preterm birth (SPB) and delivery of small for gestational age (SGA) neonates, were significant predictors of fetal and maternal cfDNA in maternal plasma. Results: The fetal and maternal cfDNA plasma concentration increased with serum pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin level, was higher in women of Afro-Caribbean and East-Asian racial origin than in Caucasians, and lower in smokers, but it was not significantly altered in pregnancies complicated by PE, SPB or SGA. The fetal cfDNA level was inversely related to maternal weight and uterine artery pulsatility index, and maternal cfDNA increased with maternal weight. Conclusions: The fetal and maternal cfDNA level in maternal plasma is affected by maternal and fetal characteristics, but it is not altered in pregnancy complications.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Cohort Studies</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>DNA - blood</subject><subject>Early Diagnosis</subject><subject>Female</subject><subject>Fetal Growth Retardation - blood</subject><subject>Fetal Growth Retardation - diagnosis</subject><subject>Fetal Growth Retardation - physiopathology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Original Paper</subject><subject>Placentation</subject><subject>Plasma - metabolism</subject><subject>Pre-Eclampsia - blood</subject><subject>Pre-Eclampsia - diagnosis</subject><subject>Pre-Eclampsia - physiopathology</subject><subject>Pregnancy</subject><subject>Pregnancy Complications - blood</subject><subject>Pregnancy Complications - diagnosis</subject><subject>Pregnancy Complications - physiopathology</subject><subject>Pregnancy Trimester, First</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Premature Birth - blood</subject><subject>Premature Birth - diagnosis</subject><subject>Premature Birth - physiopathology</subject><subject>Prenatal Diagnosis</subject><subject>Prospective Studies</subject><subject>Pulsatile Flow</subject><subject>Uterine Artery - physiology</subject><subject>Uterine Artery - physiopathology</subject><subject>Weight Loss</subject><issn>1015-3837</issn><issn>1421-9964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0c1PFTEQAPDGSORDD96NaWIMcFidbrv98EYePCBBIQTjcdPtzuLCfmDbPfBn8B_Tx3s-E0Oip07bX6eZGULeMvjEWGE-AwAXUoN8QbaYyFlmjBQvUwysyLjmapNsh3CTmFZcviKbeeJS8HyLPHy1Ef1gO3rR2dBbOsOuy-Yekc4xpmM71HRtDr8dUBspYxnj9Afibdilxxiije04fKGX2D1FNI7PvZ79tN66tGlDbF14urzweD3Ywd3T8ym6scfwmmw0tgv4ZrXukO_zo6vZSXZ2fnw6OzjLnJBFzPJGQAW84NxWTvGqUg1vcqdAiMYIVhlwiLUwCJjX2gCrcl0Z3WittBCi5jtkb5n3zo-_plRE2bfBpertgOMUSpbeqIIbDv-m3BgDEnLzH7SQXJrCLOiHv-jNOC0alZRgRmlIKKn9pXJ-DMFjU975trf-vmRQLqZfrqef7PtVxqnqsV7L3-NO4OMK2OBs1_jU-Tb8cUqkKuQi0bulu7X-Gv0arP55BFzPvF0</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Poon, L.C.Y.</creator><creator>Musci, T.</creator><creator>Song, K.</creator><creator>Syngelaki, A.</creator><creator>Nicolaides, K.H.</creator><general>Karger</general><general>S. 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Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Original Paper</topic><topic>Placentation</topic><topic>Plasma - metabolism</topic><topic>Pre-Eclampsia - blood</topic><topic>Pre-Eclampsia - diagnosis</topic><topic>Pre-Eclampsia - physiopathology</topic><topic>Pregnancy</topic><topic>Pregnancy Complications - blood</topic><topic>Pregnancy Complications - diagnosis</topic><topic>Pregnancy Complications - physiopathology</topic><topic>Pregnancy Trimester, First</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Premature Birth - blood</topic><topic>Premature Birth - diagnosis</topic><topic>Premature Birth - physiopathology</topic><topic>Prenatal Diagnosis</topic><topic>Prospective Studies</topic><topic>Pulsatile Flow</topic><topic>Uterine Artery - physiology</topic><topic>Uterine Artery - physiopathology</topic><topic>Weight Loss</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poon, L.C.Y.</creatorcontrib><creatorcontrib>Musci, T.</creatorcontrib><creatorcontrib>Song, K.</creatorcontrib><creatorcontrib>Syngelaki, A.</creatorcontrib><creatorcontrib>Nicolaides, K.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Fetal diagnosis and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poon, L.C.Y.</au><au>Musci, T.</au><au>Song, K.</au><au>Syngelaki, A.</au><au>Nicolaides, K.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes</atitle><jtitle>Fetal diagnosis and therapy</jtitle><addtitle>Fetal Diagn Ther</addtitle><date>2013-05</date><risdate>2013</risdate><volume>33</volume><issue>4</issue><spage>215</spage><epage>223</epage><pages>215-223</pages><issn>1015-3837</issn><eissn>1421-9964</eissn><abstract>Objective: To examine the possible relationship between maternal and fetal characteristics and pregnancy outcomes on fetal and maternal cell-free (cf) DNA in maternal plasma at 11-13 weeks' gestation. Methods: cfDNA was extracted from maternal plasma of 1,949 singleton pregnancies and chromosome-selective sequencing was used to determine the proportion of cfDNA and total cfDNA counts which was of fetal and maternal origin. Multivariate regression analysis was used to determine whether specific maternal and fetal characteristics and pregnancy complications, such as preeclampsia (PE), early spontaneous preterm birth (SPB) and delivery of small for gestational age (SGA) neonates, were significant predictors of fetal and maternal cfDNA in maternal plasma. Results: The fetal and maternal cfDNA plasma concentration increased with serum pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin level, was higher in women of Afro-Caribbean and East-Asian racial origin than in Caucasians, and lower in smokers, but it was not significantly altered in pregnancies complicated by PE, SPB or SGA. The fetal cfDNA level was inversely related to maternal weight and uterine artery pulsatility index, and maternal cfDNA increased with maternal weight. Conclusions: The fetal and maternal cfDNA level in maternal plasma is affected by maternal and fetal characteristics, but it is not altered in pregnancy complications.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>23466432</pmid><doi>10.1159/000346806</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fetal diagnosis and therapy, 2013-05, Vol.33 (4), p.215-223 |
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| source | Karger Journal Archive Collection; Karger Journals; MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Biomarkers - blood Cohort Studies Diseases of mother, fetus and pregnancy DNA - blood Early Diagnosis Female Fetal Growth Retardation - blood Fetal Growth Retardation - diagnosis Fetal Growth Retardation - physiopathology Gynecology. Andrology. Obstetrics Humans Medical sciences Original Paper Placentation Plasma - metabolism Pre-Eclampsia - blood Pre-Eclampsia - diagnosis Pre-Eclampsia - physiopathology Pregnancy Pregnancy Complications - blood Pregnancy Complications - diagnosis Pregnancy Complications - physiopathology Pregnancy Trimester, First Pregnancy. Fetus. Placenta Premature Birth - blood Premature Birth - diagnosis Premature Birth - physiopathology Prenatal Diagnosis Prospective Studies Pulsatile Flow Uterine Artery - physiology Uterine Artery - physiopathology Weight Loss |
| title | Maternal Plasma Cell-Free Fetal and Maternal DNA at 11-13 Weeks' Gestation: Relation to Fetal and Maternal Characteristics and Pregnancy Outcomes |
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