Prolonged Physical Training Decreases mRNA Levels of Glucocorticoid Receptor and Inflammatory Genes
Background/Aims: Prolonged physical exercise induces adaptive alterations in the hypothalamic-pituitary axis, increasing cortisol metabolism, and reducing cortisol synthesis and glucocorticoid sensitivity. The mechanisms responsible for this relative glucocorticoid resistance remain unknown but may...
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| Veröffentlicht in: | Hormone research in paediatrics 2010-01, Vol.74 (1), p.6-14 |
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| creator | Sousa e Silva, Tatiane Longui, Carlos Alberto Rocha, Mylene Neves Faria, Claudia Dutra Costantin Melo, Murilo Rezende Faria, Thelma Gomes de Souza, Julio Antonio Rizzo, Luiz Vicente |
| description | Background/Aims: Prolonged physical exercise induces adaptive alterations in the hypothalamic-pituitary axis, increasing cortisol metabolism, and reducing cortisol synthesis and glucocorticoid sensitivity. The mechanisms responsible for this relative glucocorticoid resistance remain unknown but may involve expression of genes encoding glucocorticoid receptor (GR) and/or inflammatory molecules of nuclear factor kappa B1 (NFkB1) signaling pathway and cytokines. This study aimed to determine the impact of prolonged physical training on the expression of genes involved in glucocorticoid action and inflammatory response. Methods: Normal sedentary male cadets of the Brazilian Air Force Academy were submitted to 6 weeks of standardized physical training. Eighteen of 29 initially selected cadets were able to fully complete the training program. Fasting glucose, insulin and cortisol levels, cytokine concentration and the expression of genes encoding GR, NFkB1, inhibitor of NFkB1 and IkB kinase A were determined before and after the training period. Results: Prolonged physical exercise reduced the basal cortisol levels and the percent cortisol reduction after dexamethasone. These findings were associated with a significant reduction in the mRNA levels of GR (6.3%), NFkB1 (63%), inhibitor of NFkB1 (25%) and IkB kinase A (46%) with concomitant reduction in cytokine concentrations (ELISA). Conclusions: Prolonged physical training decreases the glucocorticoid sensitivity and the mRNA levels of the GR gene combined with decreased mRNA of genes related to the NFkB pathway. |
| doi_str_mv | 10.1159/000313586 |
| format | Article |
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The mechanisms responsible for this relative glucocorticoid resistance remain unknown but may involve expression of genes encoding glucocorticoid receptor (GR) and/or inflammatory molecules of nuclear factor kappa B1 (NFkB1) signaling pathway and cytokines. This study aimed to determine the impact of prolonged physical training on the expression of genes involved in glucocorticoid action and inflammatory response. Methods: Normal sedentary male cadets of the Brazilian Air Force Academy were submitted to 6 weeks of standardized physical training. Eighteen of 29 initially selected cadets were able to fully complete the training program. Fasting glucose, insulin and cortisol levels, cytokine concentration and the expression of genes encoding GR, NFkB1, inhibitor of NFkB1 and IkB kinase A were determined before and after the training period. Results: Prolonged physical exercise reduced the basal cortisol levels and the percent cortisol reduction after dexamethasone. These findings were associated with a significant reduction in the mRNA levels of GR (6.3%), NFkB1 (63%), inhibitor of NFkB1 (25%) and IkB kinase A (46%) with concomitant reduction in cytokine concentrations (ELISA). Conclusions: Prolonged physical training decreases the glucocorticoid sensitivity and the mRNA levels of the GR gene combined with decreased mRNA of genes related to the NFkB pathway.</description><identifier>ISSN: 1663-2818</identifier><identifier>EISSN: 1663-2826</identifier><identifier>DOI: 10.1159/000313586</identifier><identifier>PMID: 20407229</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Adolescent ; Blood Glucose - physiology ; Body Composition - physiology ; Cytokines - genetics ; Cytokines - physiology ; Glucocorticoids - physiology ; Humans ; Hydrocortisone - physiology ; Hypothalamo-Hypophyseal System - physiology ; I-kappa B Kinase - genetics ; I-kappa B Kinase - physiology ; Insulin - blood ; Insulin - physiology ; Male ; NF-kappa B - genetics ; NF-kappa B - physiology ; Original Paper ; Physical Fitness - physiology ; Pituitary-Adrenal System - physiology ; Receptors, Glucocorticoid - biosynthesis ; Receptors, Glucocorticoid - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Statistics, Nonparametric ; Young Adult</subject><ispartof>Hormone research in paediatrics, 2010-01, Vol.74 (1), p.6-14</ispartof><rights>2010 S. Karger AG, Basel</rights><rights>Copyright 2010 S. Karger AG, Basel.</rights><rights>Copyright (c) 2010 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-5484064bf8e9d5e5d53690a8139c2107c020443e452167d8cde6daf1ba9fd3aa3</citedby><cites>FETCH-LOGICAL-c333t-5484064bf8e9d5e5d53690a8139c2107c020443e452167d8cde6daf1ba9fd3aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,2423,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20407229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sousa e Silva, Tatiane</creatorcontrib><creatorcontrib>Longui, Carlos Alberto</creatorcontrib><creatorcontrib>Rocha, Mylene Neves</creatorcontrib><creatorcontrib>Faria, Claudia Dutra Costantin</creatorcontrib><creatorcontrib>Melo, Murilo Rezende</creatorcontrib><creatorcontrib>Faria, Thelma Gomes</creatorcontrib><creatorcontrib>de Souza, Julio Antonio</creatorcontrib><creatorcontrib>Rizzo, Luiz Vicente</creatorcontrib><title>Prolonged Physical Training Decreases mRNA Levels of Glucocorticoid Receptor and Inflammatory Genes</title><title>Hormone research in paediatrics</title><addtitle>Horm Res Paediatr</addtitle><description>Background/Aims: Prolonged physical exercise induces adaptive alterations in the hypothalamic-pituitary axis, increasing cortisol metabolism, and reducing cortisol synthesis and glucocorticoid sensitivity. The mechanisms responsible for this relative glucocorticoid resistance remain unknown but may involve expression of genes encoding glucocorticoid receptor (GR) and/or inflammatory molecules of nuclear factor kappa B1 (NFkB1) signaling pathway and cytokines. This study aimed to determine the impact of prolonged physical training on the expression of genes involved in glucocorticoid action and inflammatory response. Methods: Normal sedentary male cadets of the Brazilian Air Force Academy were submitted to 6 weeks of standardized physical training. Eighteen of 29 initially selected cadets were able to fully complete the training program. Fasting glucose, insulin and cortisol levels, cytokine concentration and the expression of genes encoding GR, NFkB1, inhibitor of NFkB1 and IkB kinase A were determined before and after the training period. Results: Prolonged physical exercise reduced the basal cortisol levels and the percent cortisol reduction after dexamethasone. These findings were associated with a significant reduction in the mRNA levels of GR (6.3%), NFkB1 (63%), inhibitor of NFkB1 (25%) and IkB kinase A (46%) with concomitant reduction in cytokine concentrations (ELISA). Conclusions: Prolonged physical training decreases the glucocorticoid sensitivity and the mRNA levels of the GR gene combined with decreased mRNA of genes related to the NFkB pathway.</description><subject>Adolescent</subject><subject>Blood Glucose - physiology</subject><subject>Body Composition - physiology</subject><subject>Cytokines - genetics</subject><subject>Cytokines - physiology</subject><subject>Glucocorticoids - physiology</subject><subject>Humans</subject><subject>Hydrocortisone - physiology</subject><subject>Hypothalamo-Hypophyseal System - physiology</subject><subject>I-kappa B Kinase - genetics</subject><subject>I-kappa B Kinase - physiology</subject><subject>Insulin - blood</subject><subject>Insulin - physiology</subject><subject>Male</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - physiology</subject><subject>Original Paper</subject><subject>Physical Fitness - physiology</subject><subject>Pituitary-Adrenal System - physiology</subject><subject>Receptors, Glucocorticoid - biosynthesis</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Statistics, Nonparametric</subject><subject>Young Adult</subject><issn>1663-2818</issn><issn>1663-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpd0EFLwzAUB_AgipO5g3eRgAfxUE2aNk2OY-ocDB1jnkuWvM7OtplJK-zbm7G5g-Tw8uD3Ho8_QleUPFCaykdCCKMsFfwEXVDOWRSLmJ8e_1T00MD7Ndk5kUmanaNeTBKSxbG8QHrmbGWbFRg8-9z6UqsKL5wqm7JZ4SfQDpQHj-v52xBP4Qcqj22Bx1WnrbauLbUtDZ6Dhk1rHVaNwZOmqFRdq9Bv8Rga8JforFCVh8Gh9tHHy_Ni9BpN38eT0XAaacZYG6WJSAhPloUAaVJITcq4JEpQJnVMSaZJODthkKQx5ZkR2gA3qqBLJQvDlGJ9dLffu3H2uwPf5nXpNVSVasB2Ps8YS6WIKQvy9p9c28414bicEkG5TMIL6n6vtLPeOyjyjStr5bYB5bvs82P2wd4cNnbLGsxR_iUdwPUefCm3AncEh_lfNKaGJg</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Sousa e Silva, Tatiane</creator><creator>Longui, Carlos Alberto</creator><creator>Rocha, Mylene Neves</creator><creator>Faria, Claudia Dutra Costantin</creator><creator>Melo, Murilo Rezende</creator><creator>Faria, Thelma Gomes</creator><creator>de Souza, Julio Antonio</creator><creator>Rizzo, Luiz Vicente</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>Prolonged Physical Training Decreases mRNA Levels of Glucocorticoid Receptor and Inflammatory Genes</title><author>Sousa e Silva, Tatiane ; Longui, Carlos Alberto ; Rocha, Mylene Neves ; Faria, Claudia Dutra Costantin ; Melo, Murilo Rezende ; Faria, Thelma Gomes ; de Souza, Julio Antonio ; Rizzo, Luiz Vicente</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-5484064bf8e9d5e5d53690a8139c2107c020443e452167d8cde6daf1ba9fd3aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Blood Glucose - physiology</topic><topic>Body Composition - physiology</topic><topic>Cytokines - genetics</topic><topic>Cytokines - physiology</topic><topic>Glucocorticoids - physiology</topic><topic>Humans</topic><topic>Hydrocortisone - physiology</topic><topic>Hypothalamo-Hypophyseal System - physiology</topic><topic>I-kappa B Kinase - genetics</topic><topic>I-kappa B Kinase - physiology</topic><topic>Insulin - blood</topic><topic>Insulin - physiology</topic><topic>Male</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - physiology</topic><topic>Original Paper</topic><topic>Physical Fitness - physiology</topic><topic>Pituitary-Adrenal System - physiology</topic><topic>Receptors, Glucocorticoid - biosynthesis</topic><topic>Receptors, Glucocorticoid - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Statistics, Nonparametric</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sousa e Silva, Tatiane</creatorcontrib><creatorcontrib>Longui, Carlos Alberto</creatorcontrib><creatorcontrib>Rocha, Mylene Neves</creatorcontrib><creatorcontrib>Faria, Claudia Dutra Costantin</creatorcontrib><creatorcontrib>Melo, Murilo Rezende</creatorcontrib><creatorcontrib>Faria, Thelma Gomes</creatorcontrib><creatorcontrib>de Souza, Julio Antonio</creatorcontrib><creatorcontrib>Rizzo, Luiz Vicente</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hormone research in paediatrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sousa e Silva, Tatiane</au><au>Longui, Carlos Alberto</au><au>Rocha, Mylene Neves</au><au>Faria, Claudia Dutra Costantin</au><au>Melo, Murilo Rezende</au><au>Faria, Thelma Gomes</au><au>de Souza, Julio Antonio</au><au>Rizzo, Luiz Vicente</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prolonged Physical Training Decreases mRNA Levels of Glucocorticoid Receptor and Inflammatory Genes</atitle><jtitle>Hormone research in paediatrics</jtitle><addtitle>Horm Res Paediatr</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>74</volume><issue>1</issue><spage>6</spage><epage>14</epage><pages>6-14</pages><issn>1663-2818</issn><eissn>1663-2826</eissn><abstract>Background/Aims: Prolonged physical exercise induces adaptive alterations in the hypothalamic-pituitary axis, increasing cortisol metabolism, and reducing cortisol synthesis and glucocorticoid sensitivity. The mechanisms responsible for this relative glucocorticoid resistance remain unknown but may involve expression of genes encoding glucocorticoid receptor (GR) and/or inflammatory molecules of nuclear factor kappa B1 (NFkB1) signaling pathway and cytokines. This study aimed to determine the impact of prolonged physical training on the expression of genes involved in glucocorticoid action and inflammatory response. Methods: Normal sedentary male cadets of the Brazilian Air Force Academy were submitted to 6 weeks of standardized physical training. Eighteen of 29 initially selected cadets were able to fully complete the training program. Fasting glucose, insulin and cortisol levels, cytokine concentration and the expression of genes encoding GR, NFkB1, inhibitor of NFkB1 and IkB kinase A were determined before and after the training period. Results: Prolonged physical exercise reduced the basal cortisol levels and the percent cortisol reduction after dexamethasone. These findings were associated with a significant reduction in the mRNA levels of GR (6.3%), NFkB1 (63%), inhibitor of NFkB1 (25%) and IkB kinase A (46%) with concomitant reduction in cytokine concentrations (ELISA). Conclusions: Prolonged physical training decreases the glucocorticoid sensitivity and the mRNA levels of the GR gene combined with decreased mRNA of genes related to the NFkB pathway.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>20407229</pmid><doi>10.1159/000313586</doi><tpages>9</tpages></addata></record> |
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| subjects | Adolescent Blood Glucose - physiology Body Composition - physiology Cytokines - genetics Cytokines - physiology Glucocorticoids - physiology Humans Hydrocortisone - physiology Hypothalamo-Hypophyseal System - physiology I-kappa B Kinase - genetics I-kappa B Kinase - physiology Insulin - blood Insulin - physiology Male NF-kappa B - genetics NF-kappa B - physiology Original Paper Physical Fitness - physiology Pituitary-Adrenal System - physiology Receptors, Glucocorticoid - biosynthesis Receptors, Glucocorticoid - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Statistics, Nonparametric Young Adult |
| title | Prolonged Physical Training Decreases mRNA Levels of Glucocorticoid Receptor and Inflammatory Genes |
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