Comparison of Filtration Properties of Hepatitis B Virus, Hepatitis C Virus and Simian Virus 40 Using a Polyvinylidene Fluoride Membrane Filter
Background and Objectives: We examined the ability of a modified polyvinylidene fluoride membrane filter to remove blood–borne and surrogate viruses. Materials and Methods: Phages PR772 and PP7, hepatitis B virus (HBV), hepatitis C virus and simian virus 40 (SV40) were spiked in minimum essential me...
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Veröffentlicht in: | Vox sanguinis 1998-03, Vol.75 (3), p.181-188 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Background and Objectives: We examined the ability of a modified polyvinylidene fluoride membrane filter to remove blood–borne and surrogate viruses. Materials and Methods: Phages PR772 and PP7, hepatitis B virus (HBV), hepatitis C virus and simian virus 40 (SV40) were spiked in minimum essential media with 10% fetal calf serum and the concentration of these viruses compared before and after filtration by either plaque assay or polymerase chain reaction. Results: Viruses >50 nm were removed to below detection limits (>10 6 logs) for all filters tested. A 5–log reduction of HBV (42 nm) and 2– to 3–log reduction of HCV (30–65 nm) was observed. Conclusion: A predictable size–based removal of viral agents was observed. The results also suggest the possible utility of SV40 as a surrogate to HBV for membrane filter challenge studies. |
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ISSN: | 0042-9007 1423-0410 |
DOI: | 10.1159/000030984 |