Increased Expression of Protease M in Ovarian Tumors
Proteases are known to play important roles in tumor invasion and metastasis. Protease M, which was originally identified by Anisowicz and colleagues in 1996, is a new member of the serine protease family. We also identified the protease M transcript in a differential PCR screen of ovarian tumors an...
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| Veröffentlicht in: | Tumor biology 2001-01, Vol.22 (1), p.11-18 |
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| creator | Tanimoto, Hirotoshi Underwood, Lowell J. Shigemasa, Kazushi Parmley, Tim H. O’Brien, Timothy J. |
| description | Proteases are known to play important roles in tumor invasion and metastasis. Protease M, which was originally identified by Anisowicz and colleagues in 1996, is a new member of the serine protease family. We also identified the protease M transcript in a differential PCR screen of ovarian tumors and have investigated its expression in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labeled with 32 P and a phosphoimager was used to determine the relative expression of the protease M gene compared to internal control β-tubulin. mRNA expression levels of protease M were significantly elevated in 9 of 12 low malignant potential tumors and 30 of 32 carcinomas. Northern blot hybridization showed that the 1.7-kb protease M transcript was abundant in carcinoma but not detected in normal ovary. Immunohistochemical staining of normal overy and ovarian tumor tissue sections with antibodies generated to protease M derived peptides corroborated the semi-quantitative PCR and Northern analysis data. Our results suggest that protease M is frequently overexpressed in ovarian tumors and may therefore contribute to the invasive nature or growth capacity of ovarian carcinomas. |
| doi_str_mv | 10.1159/000030150 |
| format | Article |
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Protease M, which was originally identified by Anisowicz and colleagues in 1996, is a new member of the serine protease family. We also identified the protease M transcript in a differential PCR screen of ovarian tumors and have investigated its expression in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labeled with 32 P and a phosphoimager was used to determine the relative expression of the protease M gene compared to internal control β-tubulin. mRNA expression levels of protease M were significantly elevated in 9 of 12 low malignant potential tumors and 30 of 32 carcinomas. Northern blot hybridization showed that the 1.7-kb protease M transcript was abundant in carcinoma but not detected in normal ovary. Immunohistochemical staining of normal overy and ovarian tumor tissue sections with antibodies generated to protease M derived peptides corroborated the semi-quantitative PCR and Northern analysis data. Our results suggest that protease M is frequently overexpressed in ovarian tumors and may therefore contribute to the invasive nature or growth capacity of ovarian carcinomas.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1159/000030150</identifier><identifier>PMID: 11054022</identifier><identifier>CODEN: OBIMD4</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adenocarcinoma, Clear Cell - enzymology ; Adenocarcinoma, Clear Cell - genetics ; Adenocarcinoma, Mucinous - enzymology ; Adenocarcinoma, Mucinous - genetics ; Animals ; Antibody Specificity ; Biological and medical sciences ; Blotting, Northern ; Carcinoma - enzymology ; Carcinoma - genetics ; Carcinoma, Endometrioid - enzymology ; Carcinoma, Endometrioid - genetics ; Cystadenocarcinoma, Serous - enzymology ; Cystadenocarcinoma, Serous - genetics ; Enzyme Induction ; Female ; Female genital diseases ; Gene Expression Regulation, Neoplastic ; Gynecology. Andrology. Obstetrics ; Humans ; Immunoenzyme Techniques ; Immunoglobulin G - immunology ; Kallikreins ; Medical sciences ; Neoplasm Invasiveness ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Ovarian Neoplasms - enzymology ; Ovarian Neoplasms - genetics ; Ovary - enzymology ; Polymerase Chain Reaction ; Rabbits ; Research Article ; RNA, Messenger - analysis ; RNA, Neoplasm - analysis ; Serine Endopeptidases - biosynthesis ; Serine Endopeptidases - genetics ; Tumors</subject><ispartof>Tumor biology, 2001-01, Vol.22 (1), p.11-18</ispartof><rights>2000 S. Karger AG, Basel</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2000 S. Karger AG, Basel.</rights><rights>Copyright (c) 2001 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-c0a5d27817979951cc11ce97e83dede54fde22dadb42d0e14b56f9ca8b1b9e433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=866712$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11054022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanimoto, Hirotoshi</creatorcontrib><creatorcontrib>Underwood, Lowell J.</creatorcontrib><creatorcontrib>Shigemasa, Kazushi</creatorcontrib><creatorcontrib>Parmley, Tim H.</creatorcontrib><creatorcontrib>O’Brien, Timothy J.</creatorcontrib><title>Increased Expression of Protease M in Ovarian Tumors</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><description>Proteases are known to play important roles in tumor invasion and metastasis. Protease M, which was originally identified by Anisowicz and colleagues in 1996, is a new member of the serine protease family. We also identified the protease M transcript in a differential PCR screen of ovarian tumors and have investigated its expression in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labeled with 32 P and a phosphoimager was used to determine the relative expression of the protease M gene compared to internal control β-tubulin. mRNA expression levels of protease M were significantly elevated in 9 of 12 low malignant potential tumors and 30 of 32 carcinomas. Northern blot hybridization showed that the 1.7-kb protease M transcript was abundant in carcinoma but not detected in normal ovary. Immunohistochemical staining of normal overy and ovarian tumor tissue sections with antibodies generated to protease M derived peptides corroborated the semi-quantitative PCR and Northern analysis data. Our results suggest that protease M is frequently overexpressed in ovarian tumors and may therefore contribute to the invasive nature or growth capacity of ovarian carcinomas.</description><subject>Adenocarcinoma, Clear Cell - enzymology</subject><subject>Adenocarcinoma, Clear Cell - genetics</subject><subject>Adenocarcinoma, Mucinous - enzymology</subject><subject>Adenocarcinoma, Mucinous - genetics</subject><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Carcinoma - enzymology</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma, Endometrioid - enzymology</subject><subject>Carcinoma, Endometrioid - genetics</subject><subject>Cystadenocarcinoma, Serous - enzymology</subject><subject>Cystadenocarcinoma, Serous - genetics</subject><subject>Enzyme Induction</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology. 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Obstetrics</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin G - immunology</subject><subject>Kallikreins</subject><subject>Medical sciences</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Ovarian Neoplasms - enzymology</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovary - enzymology</subject><subject>Polymerase Chain Reaction</subject><subject>Rabbits</subject><subject>Research Article</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Neoplasm - analysis</subject><subject>Serine Endopeptidases - biosynthesis</subject><subject>Serine Endopeptidases - genetics</subject><subject>Tumors</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpd0M9LwzAUB_AgipvTg2dBioLgoZqXNG1zlDF1MJmHeS5p8iqd_TGTVfS_N6NlgrkkvHx4yfsScg70DkDIe-oXpyDoARlDxHhIeUoP_ZkCDSOW8hE5cW5NPZEyPiYjACoiytiYRPNGW1QOTTD73lh0rmyboC2CV9tud_XgJSibYPmlbKmaYNXVrXWn5KhQlcOzYZ-Qt8fZavocLpZP8-nDItRcxNtQUyUMS1JIZCKlAK0BNMoEU27QoIgKg4wZZfKIGYoQ5SIupFZpDrnEiPMJuen7bmz72aHbZnXpNFaVarDtXJb4KTgksYdX_-C67Wzj_5YxxhJGJQOPbnukbeucxSLb2LJW9icDmu1yzPY5ens5NOzyGs2fHILz4HoAymlVFVY1unR7l8ZxAjt10asPZd_R7q_7R34BAtKAFw</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Tanimoto, Hirotoshi</creator><creator>Underwood, Lowell J.</creator><creator>Shigemasa, Kazushi</creator><creator>Parmley, Tim H.</creator><creator>O’Brien, Timothy J.</creator><general>Karger</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Increased Expression of Protease M in Ovarian Tumors</title><author>Tanimoto, Hirotoshi ; Underwood, Lowell J. ; Shigemasa, Kazushi ; Parmley, Tim H. ; O’Brien, Timothy J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-c0a5d27817979951cc11ce97e83dede54fde22dadb42d0e14b56f9ca8b1b9e433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenocarcinoma, Clear Cell - enzymology</topic><topic>Adenocarcinoma, Clear Cell - genetics</topic><topic>Adenocarcinoma, Mucinous - enzymology</topic><topic>Adenocarcinoma, Mucinous - genetics</topic><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Carcinoma - enzymology</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma, Endometrioid - enzymology</topic><topic>Carcinoma, Endometrioid - genetics</topic><topic>Cystadenocarcinoma, Serous - enzymology</topic><topic>Cystadenocarcinoma, Serous - genetics</topic><topic>Enzyme Induction</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin G - immunology</topic><topic>Kallikreins</topic><topic>Medical sciences</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Ovarian Neoplasms - enzymology</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovary - enzymology</topic><topic>Polymerase Chain Reaction</topic><topic>Rabbits</topic><topic>Research Article</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Neoplasm - analysis</topic><topic>Serine Endopeptidases - biosynthesis</topic><topic>Serine Endopeptidases - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanimoto, Hirotoshi</creatorcontrib><creatorcontrib>Underwood, Lowell J.</creatorcontrib><creatorcontrib>Shigemasa, Kazushi</creatorcontrib><creatorcontrib>Parmley, Tim H.</creatorcontrib><creatorcontrib>O’Brien, Timothy J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanimoto, Hirotoshi</au><au>Underwood, Lowell J.</au><au>Shigemasa, Kazushi</au><au>Parmley, Tim H.</au><au>O’Brien, Timothy J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Expression of Protease M in Ovarian Tumors</atitle><jtitle>Tumor biology</jtitle><addtitle>Tumor Biol</addtitle><date>2001-01</date><risdate>2001</risdate><volume>22</volume><issue>1</issue><spage>11</spage><epage>18</epage><pages>11-18</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><coden>OBIMD4</coden><abstract>Proteases are known to play important roles in tumor invasion and metastasis. Protease M, which was originally identified by Anisowicz and colleagues in 1996, is a new member of the serine protease family. We also identified the protease M transcript in a differential PCR screen of ovarian tumors and have investigated its expression in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labeled with 32 P and a phosphoimager was used to determine the relative expression of the protease M gene compared to internal control β-tubulin. mRNA expression levels of protease M were significantly elevated in 9 of 12 low malignant potential tumors and 30 of 32 carcinomas. Northern blot hybridization showed that the 1.7-kb protease M transcript was abundant in carcinoma but not detected in normal ovary. Immunohistochemical staining of normal overy and ovarian tumor tissue sections with antibodies generated to protease M derived peptides corroborated the semi-quantitative PCR and Northern analysis data. Our results suggest that protease M is frequently overexpressed in ovarian tumors and may therefore contribute to the invasive nature or growth capacity of ovarian carcinomas.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>11054022</pmid><doi>10.1159/000030150</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma, Clear Cell - enzymology Adenocarcinoma, Clear Cell - genetics Adenocarcinoma, Mucinous - enzymology Adenocarcinoma, Mucinous - genetics Animals Antibody Specificity Biological and medical sciences Blotting, Northern Carcinoma - enzymology Carcinoma - genetics Carcinoma, Endometrioid - enzymology Carcinoma, Endometrioid - genetics Cystadenocarcinoma, Serous - enzymology Cystadenocarcinoma, Serous - genetics Enzyme Induction Female Female genital diseases Gene Expression Regulation, Neoplastic Gynecology. Andrology. Obstetrics Humans Immunoenzyme Techniques Immunoglobulin G - immunology Kallikreins Medical sciences Neoplasm Invasiveness Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Ovarian Neoplasms - enzymology Ovarian Neoplasms - genetics Ovary - enzymology Polymerase Chain Reaction Rabbits Research Article RNA, Messenger - analysis RNA, Neoplasm - analysis Serine Endopeptidases - biosynthesis Serine Endopeptidases - genetics Tumors |
| title | Increased Expression of Protease M in Ovarian Tumors |
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