Cloning and Sequencing of Human Oncodevelopmental Soluble Placental Tissue Protein 17 (PP17): Homology with Adipophilin and the Mouse Adipose Differentiation-Related Protein
Using a monospecific anti-PP17 antiserum, we detected 4 different molecular-weight PP17 immunoreactive proteins (31,500 kD PP17a, 48,000 kD PP17b, 60,900 kD PP17c and 74,000 kD PP17d) in different normal adult and fetal human tissues, and in term placenta, by chemiluminescence Western blot analysis....
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| Veröffentlicht in: | Tumor biology 1999-07, Vol.20 (4), p.184-192 |
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| creator | Than, Nandor G. Sumegi, Balazs Than, Gabor N. Kispal, Gyula Bohn, Hans |
| description | Using a monospecific anti-PP17 antiserum, we detected 4 different molecular-weight PP17 immunoreactive proteins (31,500 kD PP17a, 48,000 kD PP17b, 60,900 kD PP17c and 74,000 kD PP17d) in different normal adult and fetal human tissues, and in term placenta, by chemiluminescence Western blot analysis. These proteins are overexpressed in cervix carcinoma tissue. Furthermore, increased amounts of PP17b are secreted into the circulation in cervix carcinoma patients; after radical surgery, PP17b serum levels are decreased, and the protein probably has an oncodevelopmental significance. cDNAs were isolated from a human placental cDNA library with the monospecific anti-PP17 antiserum. Sequence analysis of the clones showed that they encode for the 251 residue long PP17a variant, which is identical to the previously isolated and characterized PP17 antigen described in 1983. An alignment search of the protein databank showed that PP17a is homologous to human adipophilin and mouse adipose differentiation-related protein. PP17c turned out to be a dimer of PP17a, while PP17b and PP17d immunoreactive proteins recently detected on Western blots require further investigations. |
| doi_str_mv | 10.1159/000030062 |
| format | Article |
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These proteins are overexpressed in cervix carcinoma tissue. Furthermore, increased amounts of PP17b are secreted into the circulation in cervix carcinoma patients; after radical surgery, PP17b serum levels are decreased, and the protein probably has an oncodevelopmental significance. cDNAs were isolated from a human placental cDNA library with the monospecific anti-PP17 antiserum. Sequence analysis of the clones showed that they encode for the 251 residue long PP17a variant, which is identical to the previously isolated and characterized PP17 antigen described in 1983. An alignment search of the protein databank showed that PP17a is homologous to human adipophilin and mouse adipose differentiation-related protein. PP17c turned out to be a dimer of PP17a, while PP17b and PP17d immunoreactive proteins recently detected on Western blots require further investigations.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1159/000030062</identifier><identifier>PMID: 10393528</identifier><identifier>CODEN: OBIMD4</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adult ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; Carrier Proteins ; Cloning, Molecular ; DNA, Complementary - chemistry ; DNA-Binding Proteins ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Intracellular Signaling Peptides and Proteins ; Luminescent Measurements ; Medical sciences ; Membrane Proteins ; Mice ; Molecular Sequence Data ; Original Paper ; Peptides - chemistry ; Perilipin-2 ; Perilipin-3 ; Pregnancy Proteins - chemistry ; Pregnancy Proteins - genetics ; Sequence Homology, Amino Acid ; Tumors ; Uterine Cervical Neoplasms - blood ; Vesicular Transport Proteins</subject><ispartof>Tumor biology, 1999-07, Vol.20 (4), p.184-192</ispartof><rights>1999 S. Karger AG, Basel</rights><rights>1999 INIST-CNRS</rights><rights>Copyright (c) 1999 S. 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These proteins are overexpressed in cervix carcinoma tissue. Furthermore, increased amounts of PP17b are secreted into the circulation in cervix carcinoma patients; after radical surgery, PP17b serum levels are decreased, and the protein probably has an oncodevelopmental significance. cDNAs were isolated from a human placental cDNA library with the monospecific anti-PP17 antiserum. Sequence analysis of the clones showed that they encode for the 251 residue long PP17a variant, which is identical to the previously isolated and characterized PP17 antigen described in 1983. An alignment search of the protein databank showed that PP17a is homologous to human adipophilin and mouse adipose differentiation-related protein. PP17c turned out to be a dimer of PP17a, while PP17b and PP17d immunoreactive proteins recently detected on Western blots require further investigations.</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carrier Proteins</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA-Binding Proteins</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. 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Andrology. Obstetrics</topic><topic>Humans</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Luminescent Measurements</topic><topic>Medical sciences</topic><topic>Membrane Proteins</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Original Paper</topic><topic>Peptides - chemistry</topic><topic>Perilipin-2</topic><topic>Perilipin-3</topic><topic>Pregnancy Proteins - chemistry</topic><topic>Pregnancy Proteins - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - blood</topic><topic>Vesicular Transport Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Than, Nandor G.</creatorcontrib><creatorcontrib>Sumegi, Balazs</creatorcontrib><creatorcontrib>Than, Gabor N.</creatorcontrib><creatorcontrib>Kispal, Gyula</creatorcontrib><creatorcontrib>Bohn, Hans</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Than, Nandor G.</au><au>Sumegi, Balazs</au><au>Than, Gabor N.</au><au>Kispal, Gyula</au><au>Bohn, Hans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Sequencing of Human Oncodevelopmental Soluble Placental Tissue Protein 17 (PP17): Homology with Adipophilin and the Mouse Adipose Differentiation-Related Protein</atitle><jtitle>Tumor biology</jtitle><addtitle>Tumor Biol</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>20</volume><issue>4</issue><spage>184</spage><epage>192</epage><pages>184-192</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><coden>OBIMD4</coden><abstract>Using a monospecific anti-PP17 antiserum, we detected 4 different molecular-weight PP17 immunoreactive proteins (31,500 kD PP17a, 48,000 kD PP17b, 60,900 kD PP17c and 74,000 kD PP17d) in different normal adult and fetal human tissues, and in term placenta, by chemiluminescence Western blot analysis. These proteins are overexpressed in cervix carcinoma tissue. Furthermore, increased amounts of PP17b are secreted into the circulation in cervix carcinoma patients; after radical surgery, PP17b serum levels are decreased, and the protein probably has an oncodevelopmental significance. cDNAs were isolated from a human placental cDNA library with the monospecific anti-PP17 antiserum. Sequence analysis of the clones showed that they encode for the 251 residue long PP17a variant, which is identical to the previously isolated and characterized PP17 antigen described in 1983. An alignment search of the protein databank showed that PP17a is homologous to human adipophilin and mouse adipose differentiation-related protein. PP17c turned out to be a dimer of PP17a, while PP17b and PP17d immunoreactive proteins recently detected on Western blots require further investigations.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10393528</pmid><doi>10.1159/000030062</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1010-4283 |
| ispartof | Tumor biology, 1999-07, Vol.20 (4), p.184-192 |
| issn | 1010-4283 1423-0380 |
| language | eng |
| recordid | cdi_karger_primary_30062 |
| source | MEDLINE; Karger Journals |
| subjects | Adult Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Western Carrier Proteins Cloning, Molecular DNA, Complementary - chemistry DNA-Binding Proteins Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Intracellular Signaling Peptides and Proteins Luminescent Measurements Medical sciences Membrane Proteins Mice Molecular Sequence Data Original Paper Peptides - chemistry Perilipin-2 Perilipin-3 Pregnancy Proteins - chemistry Pregnancy Proteins - genetics Sequence Homology, Amino Acid Tumors Uterine Cervical Neoplasms - blood Vesicular Transport Proteins |
| title | Cloning and Sequencing of Human Oncodevelopmental Soluble Placental Tissue Protein 17 (PP17): Homology with Adipophilin and the Mouse Adipose Differentiation-Related Protein |
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