Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-Arginine
Objectives: Our purpose was to investigate the role of nitric oxide (NO) synthase substrate L-arginine (L-arg) in the regulation of airway ciliary beat frequency (CBF). Methods: We studied the effects of L-arg on CBF of rabbit tracheal epithelial cells by using high-speed digital microscopy and dete...
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| container_title | O.R.L. Journal for oto-rhino-laryngology and its related specialties |
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| creator | Jiao, Jian Han, Demin Meng, Na Jin, Shanzhe Zhang, Luo |
| description | Objectives: Our purpose was to investigate the role of nitric oxide (NO) synthase substrate L-arginine (L-arg) in the regulation of airway ciliary beat frequency (CBF). Methods: We studied the effects of L-arg on CBF of rabbit tracheal epithelial cells by using high-speed digital microscopy and determined the expression and distribution of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in intact tracheal mucosa and in isolated cilia by using immunohistochemistry and Western blot, respectively. Results:L-arg dose- and time-dependently increased CBF compared with the control groups, and the effect was blocked by pretreatment with the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME). Immunoreactivity to 3 isoforms of NOS (nNOS, iNOS, and eNOS) was observed in cilia of epithelial cells, and the signal intensity of eNOS was stronger than that of both nNOS and iNOS. Western blot analysis of isolated ciliary preparations also detected all 3 isoforms of NOS, and the expression level is eNOS > nNOS > iNOS. Conclusion:L-arg increases the CBF of rabbit tracheal epithelial cells via the NOS-NO signal pathway. |
| doi_str_mv | 10.1159/000265683 |
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Methods: We studied the effects of L-arg on CBF of rabbit tracheal epithelial cells by using high-speed digital microscopy and determined the expression and distribution of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in intact tracheal mucosa and in isolated cilia by using immunohistochemistry and Western blot, respectively. Results:L-arg dose- and time-dependently increased CBF compared with the control groups, and the effect was blocked by pretreatment with the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME). Immunoreactivity to 3 isoforms of NOS (nNOS, iNOS, and eNOS) was observed in cilia of epithelial cells, and the signal intensity of eNOS was stronger than that of both nNOS and iNOS. Western blot analysis of isolated ciliary preparations also detected all 3 isoforms of NOS, and the expression level is eNOS > nNOS > iNOS. Conclusion:L-arg increases the CBF of rabbit tracheal epithelial cells via the NOS-NO signal pathway.</description><identifier>ISSN: 0301-1569</identifier><identifier>EISSN: 1423-0275</identifier><identifier>DOI: 10.1159/000265683</identifier><identifier>PMID: 20110742</identifier><identifier>CODEN: ORLJAH</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Amino acids ; Analysis of Variance ; Animals ; Arginine - pharmacology ; Biological and medical sciences ; Blotting, Western ; Cells, Cultured ; Cellular biology ; Cilia - drug effects ; Cilia - physiology ; Dose-Response Relationship, Drug ; Enzymes ; Immunoenzyme Techniques ; Medical sciences ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric oxide ; Nitric Oxide Synthase - physiology ; Nitric Oxide Synthase Type II - physiology ; Nitric Oxide Synthase Type III - physiology ; Original Paper ; Otorhinolaryngology. Stomatology ; Rabbits ; Signal Transduction ; Throat ; Trachea - cytology ; Trachea - enzymology</subject><ispartof>O.R.L. Journal for oto-rhino-laryngology and its related specialties, 2010-01, Vol.72 (1), p.6-11</ispartof><rights>2010 S. Karger AG, Basel</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2010 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-c1d62ca4256094ea3636b3dd148f5c9f6a35443eaab1f76023d3c6d77340d24d3</citedby><cites>FETCH-LOGICAL-c428t-c1d62ca4256094ea3636b3dd148f5c9f6a35443eaab1f76023d3c6d77340d24d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22841981$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20110742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiao, Jian</creatorcontrib><creatorcontrib>Han, Demin</creatorcontrib><creatorcontrib>Meng, Na</creatorcontrib><creatorcontrib>Jin, Shanzhe</creatorcontrib><creatorcontrib>Zhang, Luo</creatorcontrib><title>Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-Arginine</title><title>O.R.L. Journal for oto-rhino-laryngology and its related specialties</title><addtitle>ORL</addtitle><description>Objectives: Our purpose was to investigate the role of nitric oxide (NO) synthase substrate L-arginine (L-arg) in the regulation of airway ciliary beat frequency (CBF). Methods: We studied the effects of L-arg on CBF of rabbit tracheal epithelial cells by using high-speed digital microscopy and determined the expression and distribution of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in intact tracheal mucosa and in isolated cilia by using immunohistochemistry and Western blot, respectively. Results:L-arg dose- and time-dependently increased CBF compared with the control groups, and the effect was blocked by pretreatment with the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME). Immunoreactivity to 3 isoforms of NOS (nNOS, iNOS, and eNOS) was observed in cilia of epithelial cells, and the signal intensity of eNOS was stronger than that of both nNOS and iNOS. Western blot analysis of isolated ciliary preparations also detected all 3 isoforms of NOS, and the expression level is eNOS > nNOS > iNOS. Conclusion:L-arg increases the CBF of rabbit tracheal epithelial cells via the NOS-NO signal pathway.</description><subject>Amino acids</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Cilia - drug effects</subject><subject>Cilia - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzymes</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase - physiology</subject><subject>Nitric Oxide Synthase Type II - physiology</subject><subject>Nitric Oxide Synthase Type III - physiology</subject><subject>Original Paper</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Rabbits</subject><subject>Signal Transduction</subject><subject>Throat</subject><subject>Trachea - cytology</subject><subject>Trachea - enzymology</subject><issn>0301-1569</issn><issn>1423-0275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpd0MtLxDAQBvAgiq6Pg3eRIIh4qOad9qiLL1gUfJw8lGmSrtFuq0kL7n9vZFcFTzOHHzMfH0K7lJxQKotTQghTUuV8BY2oYDwjTMtVNCKc0IxKVWygzRhfE5Ms1-togxFKiRZshJ7v3XRooPddi7saPwYwLw4aPPaNhzDH5w56fBncx-BaM8fVHN_6PniD7z69dfhh3vYvENMyVLEP0Ds8yc7C1Le-ddtorYYmup3l3EJPlxeP4-tscnd1Mz6bZEawvM8MtYoZEEwqUggHXHFVcWupyGtpiloBl0JwB1DRWivCuOVGWa25IJYJy7fQ0eLue-hSztiXMx-NaxpoXTfEUnOeWpKcJnnwT752Q2hTuFJLkhIwrhM6XiATuhiDq8v34GepjJKS8rvv8rfvZPeXB4dq5uyv_Ck4gcMlgGigqQO0xsc_x3JBi_w72d7CvUGYuvAHFn--AH5Bjnc</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Jiao, Jian</creator><creator>Han, Demin</creator><creator>Meng, Na</creator><creator>Jin, Shanzhe</creator><creator>Zhang, Luo</creator><general>Karger</general><general>S. 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Stomatology</topic><topic>Rabbits</topic><topic>Signal Transduction</topic><topic>Throat</topic><topic>Trachea - cytology</topic><topic>Trachea - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiao, Jian</creatorcontrib><creatorcontrib>Han, Demin</creatorcontrib><creatorcontrib>Meng, Na</creatorcontrib><creatorcontrib>Jin, Shanzhe</creatorcontrib><creatorcontrib>Zhang, Luo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>O.R.L. Journal for oto-rhino-laryngology and its related specialties</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiao, Jian</au><au>Han, Demin</au><au>Meng, Na</au><au>Jin, Shanzhe</au><au>Zhang, Luo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-Arginine</atitle><jtitle>O.R.L. Journal for oto-rhino-laryngology and its related specialties</jtitle><addtitle>ORL</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>72</volume><issue>1</issue><spage>6</spage><epage>11</epage><pages>6-11</pages><issn>0301-1569</issn><eissn>1423-0275</eissn><coden>ORLJAH</coden><abstract>Objectives: Our purpose was to investigate the role of nitric oxide (NO) synthase substrate L-arginine (L-arg) in the regulation of airway ciliary beat frequency (CBF). Methods: We studied the effects of L-arg on CBF of rabbit tracheal epithelial cells by using high-speed digital microscopy and determined the expression and distribution of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in intact tracheal mucosa and in isolated cilia by using immunohistochemistry and Western blot, respectively. Results:L-arg dose- and time-dependently increased CBF compared with the control groups, and the effect was blocked by pretreatment with the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME). Immunoreactivity to 3 isoforms of NOS (nNOS, iNOS, and eNOS) was observed in cilia of epithelial cells, and the signal intensity of eNOS was stronger than that of both nNOS and iNOS. Western blot analysis of isolated ciliary preparations also detected all 3 isoforms of NOS, and the expression level is eNOS > nNOS > iNOS. Conclusion:L-arg increases the CBF of rabbit tracheal epithelial cells via the NOS-NO signal pathway.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>20110742</pmid><doi>10.1159/000265683</doi><tpages>6</tpages></addata></record> |
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| ispartof | O.R.L. Journal for oto-rhino-laryngology and its related specialties, 2010-01, Vol.72 (1), p.6-11 |
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| source | Karger Journals; MEDLINE; Alma/SFX Local Collection; Karger:Jisc Collections:ORL, Ophthalmology, Dental Medicine, Obstetrics, Gynecology and Psychology, Psychiatry Archive Collection (2012-2112) |
| subjects | Amino acids Analysis of Variance Animals Arginine - pharmacology Biological and medical sciences Blotting, Western Cells, Cultured Cellular biology Cilia - drug effects Cilia - physiology Dose-Response Relationship, Drug Enzymes Immunoenzyme Techniques Medical sciences NG-Nitroarginine Methyl Ester - pharmacology Nitric oxide Nitric Oxide Synthase - physiology Nitric Oxide Synthase Type II - physiology Nitric Oxide Synthase Type III - physiology Original Paper Otorhinolaryngology. Stomatology Rabbits Signal Transduction Throat Trachea - cytology Trachea - enzymology |
| title | Regulation of Tracheal Ciliary Beat Frequency by Nitric Oxide Synthase Substrate L-Arginine |
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