Expression of Tie1 and Tie2 Proteins during Reendothelialization in Balloon-Injured Rat Carotid Artery
The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the express...
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| Veröffentlicht in: | Journal of vascular research 1999-07, Vol.36 (4), p.272-281 |
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| creator | Fujikawa, Koshi Presman, Eleonora Isner, Jeffrey M. Varticovski, Lyuba |
| description | The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie2 in normal vessels and during reendothelialization following balloon injury of the adult rat carotid artery. Tie proteins were detected in quiescent endothelial cells of the adult rat carotid artery. Tie1 and Tie2 proteins were also detected in human and rat platelets. A weak expression of Tie1 and Tie2 proteins was detected in young endothelial cells which sparsely repopulated the denuded surface by day 14. Protein levels increased in the confluent layer of endothelial cells by day 28. Based on these observations, we tested whether Tie1 and Tie2 mRNA and protein levels are regulated by cell density. Tie1 and Tie2 expression significantly increased with higher density in cultured human endothelial cells, and this upregulation required cell-cell interaction. These data suggest that Tie1 and Tie2 may play a role in the maintenance and repair of the adult vascular system and that the expression of these proteins is regulated by cell density. |
| doi_str_mv | 10.1159/000025655 |
| format | Article |
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We examined the expression of Tie1 and Tie2 in normal vessels and during reendothelialization following balloon injury of the adult rat carotid artery. Tie proteins were detected in quiescent endothelial cells of the adult rat carotid artery. Tie1 and Tie2 proteins were also detected in human and rat platelets. A weak expression of Tie1 and Tie2 proteins was detected in young endothelial cells which sparsely repopulated the denuded surface by day 14. Protein levels increased in the confluent layer of endothelial cells by day 28. Based on these observations, we tested whether Tie1 and Tie2 mRNA and protein levels are regulated by cell density. Tie1 and Tie2 expression significantly increased with higher density in cultured human endothelial cells, and this upregulation required cell-cell interaction. These data suggest that Tie1 and Tie2 may play a role in the maintenance and repair of the adult vascular system and that the expression of these proteins is regulated by cell density.</description><identifier>ISSN: 1018-1172</identifier><identifier>EISSN: 1423-0135</identifier><identifier>DOI: 10.1159/000025655</identifier><identifier>PMID: 10474040</identifier><identifier>CODEN: JVREE9</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Angioplasty, Balloon - adverse effects ; Animals ; Biological and medical sciences ; Blood vessels and receptors ; Carotid Arteries - innervation ; Carotid Arteries - metabolism ; Cells, Cultured ; Endothelium, Vascular - physiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Rats ; Rats, Sprague-Dawley ; Receptor Protein-Tyrosine Kinases - analysis ; Receptor, TIE-1 ; Receptors, Cell Surface - analysis ; Receptors, TIE ; Regeneration ; Research Paper ; Vertebrates: cardiovascular system</subject><ispartof>Journal of vascular research, 1999-07, Vol.36 (4), p.272-281</ispartof><rights>1999 S. Karger AG, Basel</rights><rights>1999 INIST-CNRS</rights><rights>Copyright S. 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We examined the expression of Tie1 and Tie2 in normal vessels and during reendothelialization following balloon injury of the adult rat carotid artery. Tie proteins were detected in quiescent endothelial cells of the adult rat carotid artery. Tie1 and Tie2 proteins were also detected in human and rat platelets. A weak expression of Tie1 and Tie2 proteins was detected in young endothelial cells which sparsely repopulated the denuded surface by day 14. Protein levels increased in the confluent layer of endothelial cells by day 28. Based on these observations, we tested whether Tie1 and Tie2 mRNA and protein levels are regulated by cell density. Tie1 and Tie2 expression significantly increased with higher density in cultured human endothelial cells, and this upregulation required cell-cell interaction. 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Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor Protein-Tyrosine Kinases - analysis</subject><subject>Receptor, TIE-1</subject><subject>Receptors, Cell Surface - analysis</subject><subject>Receptors, TIE</subject><subject>Regeneration</subject><subject>Research Paper</subject><subject>Vertebrates: cardiovascular system</subject><issn>1018-1172</issn><issn>1423-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpt0MuLFDEQB-AgivvQg2dBwrIIHlorr34c12HVlQVlGLw21d2VNWNPMpt0g-tfb8YZVxFzSaC-qhQ_xp4JeC2Ead5APtKUxjxgx0JLVYBQ5mF-g6gLISp5xE5SWgMI3dTlY3YkQFcaNBwze_l9GyklFzwPlq8cCY5-2D0k_xzDRM4nPszR-Ru-JPJDmL7S6HB0P3DadTnP3-I4huCLK7-eIw18iRNfYG52A7-IE8W7J-yRxTHR08N9ylbvLleLD8X1p_dXi4vrole1nopG9BpM1emurqUqRdlJQIuy7KgEIzuNlVUNKIuDFIRaglagelkj2t4adcpe7sduY7idKU3txqWexhE9hTm1Vc4JjCkzPPsHrsMcfV6tlVIbIysjMnq1R30MKUWy7Ta6Dca7VkC7C769Dz7bF4eBc7eh4S-5TzqD8wPA1ONoI_repT-uyXtVu8We79k3jDcU7-u_fzn7b_Xjl-Uv0G4Hq34CaLWdZg</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Fujikawa, Koshi</creator><creator>Presman, Eleonora</creator><creator>Isner, Jeffrey M.</creator><creator>Varticovski, Lyuba</creator><general>Karger</general><general>S. 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Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor Protein-Tyrosine Kinases - analysis</topic><topic>Receptor, TIE-1</topic><topic>Receptors, Cell Surface - analysis</topic><topic>Receptors, TIE</topic><topic>Regeneration</topic><topic>Research Paper</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujikawa, Koshi</creatorcontrib><creatorcontrib>Presman, Eleonora</creatorcontrib><creatorcontrib>Isner, Jeffrey M.</creatorcontrib><creatorcontrib>Varticovski, Lyuba</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of vascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujikawa, Koshi</au><au>Presman, Eleonora</au><au>Isner, Jeffrey M.</au><au>Varticovski, Lyuba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Tie1 and Tie2 Proteins during Reendothelialization in Balloon-Injured Rat Carotid Artery</atitle><jtitle>Journal of vascular research</jtitle><addtitle>J Vasc Res</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>36</volume><issue>4</issue><spage>272</spage><epage>281</epage><pages>272-281</pages><issn>1018-1172</issn><eissn>1423-0135</eissn><coden>JVREE9</coden><abstract>The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie2 in normal vessels and during reendothelialization following balloon injury of the adult rat carotid artery. Tie proteins were detected in quiescent endothelial cells of the adult rat carotid artery. Tie1 and Tie2 proteins were also detected in human and rat platelets. A weak expression of Tie1 and Tie2 proteins was detected in young endothelial cells which sparsely repopulated the denuded surface by day 14. Protein levels increased in the confluent layer of endothelial cells by day 28. Based on these observations, we tested whether Tie1 and Tie2 mRNA and protein levels are regulated by cell density. Tie1 and Tie2 expression significantly increased with higher density in cultured human endothelial cells, and this upregulation required cell-cell interaction. These data suggest that Tie1 and Tie2 may play a role in the maintenance and repair of the adult vascular system and that the expression of these proteins is regulated by cell density.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10474040</pmid><doi>10.1159/000025655</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of vascular research, 1999-07, Vol.36 (4), p.272-281 |
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| subjects | Angioplasty, Balloon - adverse effects Animals Biological and medical sciences Blood vessels and receptors Carotid Arteries - innervation Carotid Arteries - metabolism Cells, Cultured Endothelium, Vascular - physiology Fundamental and applied biological sciences. Psychology Humans Male Rats Rats, Sprague-Dawley Receptor Protein-Tyrosine Kinases - analysis Receptor, TIE-1 Receptors, Cell Surface - analysis Receptors, TIE Regeneration Research Paper Vertebrates: cardiovascular system |
| title | Expression of Tie1 and Tie2 Proteins during Reendothelialization in Balloon-Injured Rat Carotid Artery |
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