In vitro Synthesis of 1α ,25-Dihydroxycholecalciferoland 24,25-Dihydroxycholecalciferol by Isolated Calvarial Cells

The question of whether the skeleton metabolizes 25-hydroxycholecalciferol [25(OH)D3] to more-polar products was studied. Calvarial cells were dispersed from 16-day old chicken embryos by using collagenase and then grown in culture in serum-free medium. Confluent cell cultures were incubated with 7...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1980-10, Vol.77 (10), p.5720-5724
Hauptverfasser:	Turner, Russell T., Puzas, J. Edward, Forte, Mario D., Lester, Gayle E., Gray, T. Kenney, Howard, Guy A., Baylink, David J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell culture techniques Cell separation Cellular metabolism Cultured cells Embryos Endothelial cells Kidney cells Kidneys Skull Vitamin D
Online-Zugang:	Volltext
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