Epidermal Growth Factor-Induced Down-Regulation of Receptor Does Not Occur in HeLa Cells Grown in Defined Medium
125I-Labeled epidermal growth factor (EGF) binds to HeLa cells in culture under completely defined conditions in a specific and saturable manner. When the cultures are maintained in serum, all the reported phenomena of down-regulation occur. In contrast, in cultures maintained in serum-free hormone-...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-05, Vol.77 (5), p.2735-2739 |
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| container_issue | 5 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 77 |
| creator | Wolfe, R. A. Wu, R. Sato, G. H. |
| description | 125I-Labeled epidermal growth factor (EGF) binds to HeLa cells in culture under completely defined conditions in a specific and saturable manner. When the cultures are maintained in serum, all the reported phenomena of down-regulation occur. In contrast, in cultures maintained in serum-free hormone-supplemented medium, binding is maximal after 50 min and remains constant for at least 18 hr. EGF retains its mitogenic activity in this defined system; as little as 4 pM EGF elicits a 2-fold increase in cell number in 5 days. EGF appears to be in reversible equilibrium with a constant number of surface receptors, and gel filtration analysis indicates a decrease in the rate of degradation and release of bound125I-labeled EGF. These results indicate that EGF can retain its mitogenic activity in the absence of concomitant downregulation. |
| doi_str_mv | 10.1073/pnas.77.5.2735 |
| format | Article |
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A. ; Wu, R. ; Sato, G. H.</creator><creatorcontrib>Wolfe, R. A. ; Wu, R. ; Sato, G. H.</creatorcontrib><description>125I-Labeled epidermal growth factor (EGF) binds to HeLa cells in culture under completely defined conditions in a specific and saturable manner. When the cultures are maintained in serum, all the reported phenomena of down-regulation occur. In contrast, in cultures maintained in serum-free hormone-supplemented medium, binding is maximal after 50 min and remains constant for at least 18 hr. EGF retains its mitogenic activity in this defined system; as little as 4 pM EGF elicits a 2-fold increase in cell number in 5 days. EGF appears to be in reversible equilibrium with a constant number of surface receptors, and gel filtration analysis indicates a decrease in the rate of degradation and release of bound125I-labeled EGF. These results indicate that EGF can retain its mitogenic activity in the absence of concomitant downregulation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.5.2735</identifier><identifier>PMID: 6248863</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Cell Division - drug effects ; Cell growth ; Cells ; Culture Media ; Cultured cells ; Dose-Response Relationship, Drug ; Down regulation ; Epidermal Growth Factor - metabolism ; Epidermal Growth Factor - pharmacology ; Gels ; HeLa cells ; HeLa Cells - drug effects ; HeLa Cells - metabolism ; Hormones ; Humans ; Kinetics ; Ligands ; Molecular Weight ; Peptides - pharmacology ; Radioactive decay ; Receptors ; Receptors, Cell Surface - drug effects ; Receptors, Cell Surface - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-05, Vol.77 (5), p.2735-2739</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-3abef2cdb4daa74564e768d9c75725d268ca325b29355a80b77cb9f045d31b323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/8768$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/8768$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6248863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolfe, R. A.</creatorcontrib><creatorcontrib>Wu, R.</creatorcontrib><creatorcontrib>Sato, G. H.</creatorcontrib><title>Epidermal Growth Factor-Induced Down-Regulation of Receptor Does Not Occur in HeLa Cells Grown in Defined Medium</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>125I-Labeled epidermal growth factor (EGF) binds to HeLa cells in culture under completely defined conditions in a specific and saturable manner. When the cultures are maintained in serum, all the reported phenomena of down-regulation occur. In contrast, in cultures maintained in serum-free hormone-supplemented medium, binding is maximal after 50 min and remains constant for at least 18 hr. EGF retains its mitogenic activity in this defined system; as little as 4 pM EGF elicits a 2-fold increase in cell number in 5 days. EGF appears to be in reversible equilibrium with a constant number of surface receptors, and gel filtration analysis indicates a decrease in the rate of degradation and release of bound125I-labeled EGF. These results indicate that EGF can retain its mitogenic activity in the absence of concomitant downregulation.</description><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cells</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down regulation</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Gels</subject><subject>HeLa cells</subject><subject>HeLa Cells - drug effects</subject><subject>HeLa Cells - metabolism</subject><subject>Hormones</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Ligands</subject><subject>Molecular Weight</subject><subject>Peptides - pharmacology</subject><subject>Radioactive decay</subject><subject>Receptors</subject><subject>Receptors, Cell Surface - drug effects</subject><subject>Receptors, Cell Surface - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtv1DAUhS0EKkNhi8QCySt2CY4fsbNggaZPaaBSBWvLsW_aVBk72EkL_x6HGUaDxMqSz3fOvfZB6G1FyopI9nH0JpVSlqKkkolnaFWRpipq3pDnaEUIlYXilL9Er1J6IIQ0QpETdFJTrlTNVmg8H3sHcWsGfBnD03SPL4ydQiyuvZstOHwWnnxxC3fzYKY-eBw6fAsWxsxkDRL-GiZ8Y-0cce_xFWwMXsMwpD9xfrk7g673OekLuH7evkYvOjMkeLM_T9H3i_Nv66tic3N5vf68KSwXYiqYaaGj1rXcGSO5qDnIWrnGSiGpcLRW1jAqWtowIYwirZS2bTrChWNVyyg7RZ92uePcbsFZ8FM0gx5jvzXxlw6m1_8qvr_Xd-FRM95wqbL_w94fw48Z0qS3fbL5ZcZDmJOWopKqoiSD5Q60MaQUoTvMqIheKtJLRVpKLfRSUTa8P97sgO87OZq8-P6qB7_u5mGY4Od0FPRfMOvvdvpDym0dAJU_kv0GpsCurw</recordid><startdate>19800501</startdate><enddate>19800501</enddate><creator>Wolfe, R. A.</creator><creator>Wu, R.</creator><creator>Sato, G. H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800501</creationdate><title>Epidermal Growth Factor-Induced Down-Regulation of Receptor Does Not Occur in HeLa Cells Grown in Defined Medium</title><author>Wolfe, R. A. ; Wu, R. ; Sato, G. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-3abef2cdb4daa74564e768d9c75725d268ca325b29355a80b77cb9f045d31b323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cells</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Dose-Response Relationship, Drug</topic><topic>Down regulation</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Gels</topic><topic>HeLa cells</topic><topic>HeLa Cells - drug effects</topic><topic>HeLa Cells - metabolism</topic><topic>Hormones</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Ligands</topic><topic>Molecular Weight</topic><topic>Peptides - pharmacology</topic><topic>Radioactive decay</topic><topic>Receptors</topic><topic>Receptors, Cell Surface - drug effects</topic><topic>Receptors, Cell Surface - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolfe, R. A.</creatorcontrib><creatorcontrib>Wu, R.</creatorcontrib><creatorcontrib>Sato, G. H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolfe, R. A.</au><au>Wu, R.</au><au>Sato, G. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidermal Growth Factor-Induced Down-Regulation of Receptor Does Not Occur in HeLa Cells Grown in Defined Medium</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-05-01</date><risdate>1980</risdate><volume>77</volume><issue>5</issue><spage>2735</spage><epage>2739</epage><pages>2735-2739</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>125I-Labeled epidermal growth factor (EGF) binds to HeLa cells in culture under completely defined conditions in a specific and saturable manner. When the cultures are maintained in serum, all the reported phenomena of down-regulation occur. In contrast, in cultures maintained in serum-free hormone-supplemented medium, binding is maximal after 50 min and remains constant for at least 18 hr. EGF retains its mitogenic activity in this defined system; as little as 4 pM EGF elicits a 2-fold increase in cell number in 5 days. EGF appears to be in reversible equilibrium with a constant number of surface receptors, and gel filtration analysis indicates a decrease in the rate of degradation and release of bound125I-labeled EGF. These results indicate that EGF can retain its mitogenic activity in the absence of concomitant downregulation.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6248863</pmid><doi>10.1073/pnas.77.5.2735</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1980-05, Vol.77 (5), p.2735-2739 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Open Access: PubMed Central; MEDLINE; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR |
| subjects | Cell Division - drug effects Cell growth Cells Culture Media Cultured cells Dose-Response Relationship, Drug Down regulation Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology Gels HeLa cells HeLa Cells - drug effects HeLa Cells - metabolism Hormones Humans Kinetics Ligands Molecular Weight Peptides - pharmacology Radioactive decay Receptors Receptors, Cell Surface - drug effects Receptors, Cell Surface - metabolism |
| title | Epidermal Growth Factor-Induced Down-Regulation of Receptor Does Not Occur in HeLa Cells Grown in Defined Medium |
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