Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR
Real-time solvent exchange measurements using Fourier transform NMR at 270 MHz are presented. By means of the fast gel filtration column techniques originally developed for tritium exchange experiments, we were able to replace the solvent of a tRNA sample from an1H2O to an2H2O buffer and obtain a us...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-07, Vol.76 (7), p.3130-3134 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Johnston, P. D. Figueroa, N. Redfield, A. G. |
| description | Real-time solvent exchange measurements using Fourier transform NMR at 270 MHz are presented. By means of the fast gel filtration column techniques originally developed for tritium exchange experiments, we were able to replace the solvent of a tRNA sample from an1H2O to an2H2O buffer and obtain a useful spectrum in 2-5 min. At 15 degrees C, there are 5 ± 1 lowfield (-11 to -15 ppm relative to 2,2-dimethyl-2-silapentane-5-sulfonate) imino protons with exchange half times of minutes to hours. In addition, the m7G-46 C(8) proton and several amino protons are observed to exchange with similar rates. Analogous studies on unfractionated yeast tRNA suggest that such a class of slowly exchanging imino protons is present in several tRNAs, and that the activation energy for exchange is small [≈ 5 kcal/mol (21 kJ/mol)]. We speculate that these imino resonances arise from D-stem protons and that their slow exchange reflects stabilization by the numerous tertiary interactions involving this stem and the Mg2+bound at the P-10 bend. |
| doi_str_mv | 10.1073/pnas.76.7.3130 |
| format | Article |
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D. ; Figueroa, N. ; Redfield, A. G.</creator><creatorcontrib>Johnston, P. D. ; Figueroa, N. ; Redfield, A. G. ; Menoufia Univ. (Egypt)</creatorcontrib><description>Real-time solvent exchange measurements using Fourier transform NMR at 270 MHz are presented. By means of the fast gel filtration column techniques originally developed for tritium exchange experiments, we were able to replace the solvent of a tRNA sample from an1H2O to an2H2O buffer and obtain a useful spectrum in 2-5 min. At 15 degrees C, there are 5 ± 1 lowfield (-11 to -15 ppm relative to 2,2-dimethyl-2-silapentane-5-sulfonate) imino protons with exchange half times of minutes to hours. In addition, the m7G-46 C(8) proton and several amino protons are observed to exchange with similar rates. Analogous studies on unfractionated yeast tRNA suggest that such a class of slowly exchanging imino protons is present in several tRNAs, and that the activation energy for exchange is small [≈ 5 kcal/mol (21 kJ/mol)]. We speculate that these imino resonances arise from D-stem protons and that their slow exchange reflects stabilization by the numerous tertiary interactions involving this stem and the Mg2+bound at the P-10 bend.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.76.7.3130</identifier><identifier>PMID: 386331</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Base Composition ; Biochemistry ; Exchange rates ; Fourier Analysis ; Hydrogen ; Hydrogen Bonding ; Low temperature ; Magnetic Resonance Spectroscopy ; Melting ; Nucleic Acid Conformation ; Nucleic acids ; Phenylalanine ; Protons ; RNA, Fungal ; RNA, Transfer, Amino Acyl ; Saccharomyces cerevisiae - metabolism ; Solvents ; Transfer RNA ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1979-07, Vol.76 (7), p.3130-3134</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-ac210f1a5b9afd77d6aa0bb385e7ed651fac7bb2c39807fcfe611f8252657f123</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/76/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/69943$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/69943$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/386331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnston, P. D.</creatorcontrib><creatorcontrib>Figueroa, N.</creatorcontrib><creatorcontrib>Redfield, A. G.</creatorcontrib><creatorcontrib>Menoufia Univ. (Egypt)</creatorcontrib><title>Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Real-time solvent exchange measurements using Fourier transform NMR at 270 MHz are presented. By means of the fast gel filtration column techniques originally developed for tritium exchange experiments, we were able to replace the solvent of a tRNA sample from an1H2O to an2H2O buffer and obtain a useful spectrum in 2-5 min. At 15 degrees C, there are 5 ± 1 lowfield (-11 to -15 ppm relative to 2,2-dimethyl-2-silapentane-5-sulfonate) imino protons with exchange half times of minutes to hours. In addition, the m7G-46 C(8) proton and several amino protons are observed to exchange with similar rates. Analogous studies on unfractionated yeast tRNA suggest that such a class of slowly exchanging imino protons is present in several tRNAs, and that the activation energy for exchange is small [≈ 5 kcal/mol (21 kJ/mol)]. We speculate that these imino resonances arise from D-stem protons and that their slow exchange reflects stabilization by the numerous tertiary interactions involving this stem and the Mg2+bound at the P-10 bend.</description><subject>Base Composition</subject><subject>Biochemistry</subject><subject>Exchange rates</subject><subject>Fourier Analysis</subject><subject>Hydrogen</subject><subject>Hydrogen Bonding</subject><subject>Low temperature</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Melting</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Phenylalanine</subject><subject>Protons</subject><subject>RNA, Fungal</subject><subject>RNA, Transfer, Amino Acyl</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Solvents</subject><subject>Transfer RNA</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS3E11K4ckCAfOKWYMeJnRw4VFULSKVICz1bk8TedZXYqe1U3Qt_e71NWZULJ9vze8_j8UPoLSU5JYJ9niyEXPBc5Iwy8gStKGloxsuGPEUrQgqR1WVRvkSvQrgihDRVTV6g56zmjNEV-rNWMGTRjAoHN9woG7G67bZgN6kQ596ogJ3GcauwGY11GGyP4X43eRedvcc7BSHiaavsboABrLEKRw82aOXx-uIYtzt85mZv0nGpOz_iix_r1-iZhiGoNw_rEbo8O_198i07__n1-8nxedaVoo4ZdAUlmkLVNqB7IXoOQNqW1ZUSqucV1dCJti061tRE6E4rTqmui6rgldC0YEfoy3LvNLej6rs0p4dBTt6M4HfSgZH_Emu2cuNuJKuZECL5Pz34vbueVYhyNKFTQ5pVuTlIUQpOOKuSMF-EnXcheKUPPSiR-7zkPi8puBRyn1cyvH_8soN8CehR473tLzzYpZ6HIarbmIQf_idM_N3Cr0J0_iDgTVOyBD8uUIOTsPEmyMtfNP0lqYq6LgW7A7gtvqQ</recordid><startdate>19790701</startdate><enddate>19790701</enddate><creator>Johnston, P. D.</creator><creator>Figueroa, N.</creator><creator>Redfield, A. G.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790701</creationdate><title>Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR</title><author>Johnston, P. D. ; Figueroa, N. ; Redfield, A. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-ac210f1a5b9afd77d6aa0bb385e7ed651fac7bb2c39807fcfe611f8252657f123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Base Composition</topic><topic>Biochemistry</topic><topic>Exchange rates</topic><topic>Fourier Analysis</topic><topic>Hydrogen</topic><topic>Hydrogen Bonding</topic><topic>Low temperature</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Melting</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic acids</topic><topic>Phenylalanine</topic><topic>Protons</topic><topic>RNA, Fungal</topic><topic>RNA, Transfer, Amino Acyl</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Solvents</topic><topic>Transfer RNA</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnston, P. D.</creatorcontrib><creatorcontrib>Figueroa, N.</creatorcontrib><creatorcontrib>Redfield, A. G.</creatorcontrib><creatorcontrib>Menoufia Univ. (Egypt)</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnston, P. D.</au><au>Figueroa, N.</au><au>Redfield, A. G.</au><aucorp>Menoufia Univ. (Egypt)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-07-01</date><risdate>1979</risdate><volume>76</volume><issue>7</issue><spage>3130</spage><epage>3134</epage><pages>3130-3134</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Real-time solvent exchange measurements using Fourier transform NMR at 270 MHz are presented. By means of the fast gel filtration column techniques originally developed for tritium exchange experiments, we were able to replace the solvent of a tRNA sample from an1H2O to an2H2O buffer and obtain a useful spectrum in 2-5 min. At 15 degrees C, there are 5 ± 1 lowfield (-11 to -15 ppm relative to 2,2-dimethyl-2-silapentane-5-sulfonate) imino protons with exchange half times of minutes to hours. In addition, the m7G-46 C(8) proton and several amino protons are observed to exchange with similar rates. Analogous studies on unfractionated yeast tRNA suggest that such a class of slowly exchanging imino protons is present in several tRNAs, and that the activation energy for exchange is small [≈ 5 kcal/mol (21 kJ/mol)]. We speculate that these imino resonances arise from D-stem protons and that their slow exchange reflects stabilization by the numerous tertiary interactions involving this stem and the Mg2+bound at the P-10 bend.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>386331</pmid><doi>10.1073/pnas.76.7.3130</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Base Composition Biochemistry Exchange rates Fourier Analysis Hydrogen Hydrogen Bonding Low temperature Magnetic Resonance Spectroscopy Melting Nucleic Acid Conformation Nucleic acids Phenylalanine Protons RNA, Fungal RNA, Transfer, Amino Acyl Saccharomyces cerevisiae - metabolism Solvents Transfer RNA Yeasts |
| title | Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR |
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