Antigen-Specific Identification and Cloning of Hybridomas with a Fluorescence-Activated Cell Sorter

Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. The ratio of specific to nonspecific microsphere binding by viable hybridoma cells was about 100:1. By using a modified...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1979-04, Vol.76 (4), p.1962-1966
Hauptverfasser:	Parks, David R., Bryan, Virginia M., Oi, Vernon T., Herzenberg, Leonard A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibodies, Neoplasm Antibody Formation Antigens Antigens, Neoplasm B lymphocytes Cell Line Cell lines Cells Clone Cells Feeder cells Fluorescence Hybrid cells Hybrid Cells - immunology Hybridomas Light Mice Myeloma Proteins Plasmacytoma Scattering, Radiation Spectrometry, Fluorescence Spleen
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