Antigen-Specific Identification and Cloning of Hybridomas with a Fluorescence-Activated Cell Sorter
Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. The ratio of specific to nonspecific microsphere binding by viable hybridoma cells was about 100:1. By using a modified...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-04, Vol.76 (4), p.1962-1966 |
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| container_end_page | 1966 |
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| container_issue | 4 |
| container_start_page | 1962 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 76 |
| creator | Parks, David R. Bryan, Virginia M. Oi, Vernon T. Herzenberg, Leonard A. |
| description | Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. The ratio of specific to nonspecific microsphere binding by viable hybridoma cells was about 100:1. By using a modified fluorescence-activated cell sorter (FACS), selected hybridoma cells in a mixture have been sorted individually into media in microculture wells, where, with thymocyte feeder cells, they developed into clones producing a desired monoclonal antibody. Viable cells were selected by measurement of their light scattering and autofluorescence properties. Rare antibody-producing clones were obtained without laborious screening and repeated subculturing. This technique should expand the range of monoclonal antibodies readily obtained from hybridomas and greatly facilitate the process of obtaining desired hybridomas. |
| doi_str_mv | 10.1073/pnas.76.4.1962 |
| format | Article |
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This technique should expand the range of monoclonal antibodies readily obtained from hybridomas and greatly facilitate the process of obtaining desired hybridomas.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Neoplasm</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Antigens, Neoplasm</subject><subject>B lymphocytes</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Clone Cells</subject><subject>Feeder cells</subject><subject>Fluorescence</subject><subject>Hybrid cells</subject><subject>Hybrid Cells - immunology</subject><subject>Hybridomas</subject><subject>Light</subject><subject>Mice</subject><subject>Myeloma Proteins</subject><subject>Plasmacytoma</subject><subject>Scattering, Radiation</subject><subject>Spectrometry, Fluorescence</subject><subject>Spleen</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kT1vGyEAhlHVLyft2qFqpZuy3YWvg2PIYFlNEylSh6Qz4jhwiDC4wKXNvy-WXStdMoF43oevF4BPCHYIcnK-DSp3nHW0Q4LhV2CBoEAtowK-BgsIMW8Hiul7cJLzA4RQ9AN8B97igUPCF0AvQ3FrE9rbrdHOOt1cT6Yu1ZkqLoZGhalZ-RhcWDfRNldPY3JT3Kjc_HblvlHNpZ9jMlmboE271MU9qmKqY7xvbmMqJn0Ab6zy2Xw8jKfg5-W3u9VVe_Pj-_VqedNq2vel1dCMBPbIWIWHiWPG8WBHNYyQYzERwjBVDFklsBgnPmI7WNKPRBFIKxeGnIKL_b7bedyYqd6oJOXlNrmNSk8yKif_J8Hdy3V8lGQgPSLVPzv4Kf6aTS5y4-q7vFfBxDlLThkc6r_WYLcP6hRzTsYez0BQ7kqRu1IkZ5LKXSlV-PL8Zsf4voWKvx7wTvsHn-tnL3FpZ--L-VNq8PM--JBLTMckE4z05C80zqr2</recordid><startdate>19790401</startdate><enddate>19790401</enddate><creator>Parks, David R.</creator><creator>Bryan, Virginia M.</creator><creator>Oi, Vernon T.</creator><creator>Herzenberg, Leonard A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790401</creationdate><title>Antigen-Specific Identification and Cloning of Hybridomas with a Fluorescence-Activated Cell Sorter</title><author>Parks, David R. ; Bryan, Virginia M. ; Oi, Vernon T. ; Herzenberg, Leonard A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-c0eb3051efa28d726728fba8b0729d33624a61fa929bd7b2f8f35b3a30429d9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Neoplasm</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Antigens, Neoplasm</topic><topic>B lymphocytes</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cells</topic><topic>Clone Cells</topic><topic>Feeder cells</topic><topic>Fluorescence</topic><topic>Hybrid cells</topic><topic>Hybrid Cells - immunology</topic><topic>Hybridomas</topic><topic>Light</topic><topic>Mice</topic><topic>Myeloma Proteins</topic><topic>Plasmacytoma</topic><topic>Scattering, Radiation</topic><topic>Spectrometry, Fluorescence</topic><topic>Spleen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parks, David R.</creatorcontrib><creatorcontrib>Bryan, Virginia M.</creatorcontrib><creatorcontrib>Oi, Vernon T.</creatorcontrib><creatorcontrib>Herzenberg, Leonard A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parks, David R.</au><au>Bryan, Virginia M.</au><au>Oi, Vernon T.</au><au>Herzenberg, Leonard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigen-Specific Identification and Cloning of Hybridomas with a Fluorescence-Activated Cell Sorter</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-04-01</date><risdate>1979</risdate><volume>76</volume><issue>4</issue><spage>1962</spage><epage>1966</epage><pages>1962-1966</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-04, Vol.76 (4), p.1962-1966 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_69635 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antibodies, Neoplasm Antibody Formation Antigens Antigens, Neoplasm B lymphocytes Cell Line Cell lines Cells Clone Cells Feeder cells Fluorescence Hybrid cells Hybrid Cells - immunology Hybridomas Light Mice Myeloma Proteins Plasmacytoma Scattering, Radiation Spectrometry, Fluorescence Spleen |
| title | Antigen-Specific Identification and Cloning of Hybridomas with a Fluorescence-Activated Cell Sorter |
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