Enkephalin Inhibits Release of Substance P from Sensory Neurons in Culture and Decreases Action Potential Duration
Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephali...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-01, Vol.76 (1), p.526-530 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 76 |
| creator | Mudge, Anne W. Leeman, Susan E. Fischbach, Gerald D. |
| description | Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 μ M inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release. |
| doi_str_mv | 10.1073/pnas.76.1.526 |
| format | Article |
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When depolarized in high-K+media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 μ M inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. 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When depolarized in high-K+media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 μ M inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release.</description><subject>Action potentials</subject><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Cell culture techniques</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Cultured cells</subject><subject>Electric Conductivity</subject><subject>Electric current</subject><subject>Endorphins - pharmacology</subject><subject>Enkephalins - pharmacology</subject><subject>Ganglia, Spinal - drug effects</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Materials</subject><subject>Membrane Potentials - drug effects</subject><subject>Nerve Endings - drug effects</subject><subject>Nerves</subject><subject>Neurons</subject><subject>Sensory neurons</subject><subject>Spinal cord</subject><subject>Substance P - metabolism</subject><subject>Synaptic Transmission - drug effects</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1v1DAQhi3E11I4ckEcfIFbFtuJ4_WBQ7UtUKmCisLZmjgTNsVrL_5A9N-TaLerInGyNM_7jEd6CXnJ2ZIzVb_beUhL1S75Uor2AVlwpnnVNpo9JAvGhKpWjWiekmcp3TDGtFyxJ-Sx4CvBmgWJ5_4n7jbgRk8v_GbsxpzoV3QICWkY6HXpUgZvkV7RIYYtvUafQryln7HE4BOdvHVxuUSk4Ht6hjbObqKnNo_B06uQ0ecRHD0rEebRc_JoAJfwxeE9Id8_nH9bf6ouv3y8WJ9eVlbyNleohQTQIBqhseUglFYaoJaNRm3lMHRQ95LzRqLtoOO8trzuVdcrzSSTQ31C3u_37kq3xd5OZ0RwZhfHLcRbE2A0_xI_bsyP8NvUK6GVnPy3Bz-GXwVTNtsxWXQOPIaSjGoaxaRWU7DaB20MKUUcjn9wZuaKzFyRUa3hZqpoyr--f9gxve9kwm8OeLbu4J1thuJcxj_53pr_5Sb8ao9vUg7xyFsta17_BWXEr6g</recordid><startdate>19790101</startdate><enddate>19790101</enddate><creator>Mudge, Anne W.</creator><creator>Leeman, Susan E.</creator><creator>Fischbach, Gerald D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790101</creationdate><title>Enkephalin Inhibits Release of Substance P from Sensory Neurons in Culture and Decreases Action Potential Duration</title><author>Mudge, Anne W. ; Leeman, Susan E. ; Fischbach, Gerald D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-e925aa9a2429e61a27979aa3549e9c5ffba3d51145ecbab113c13d7bd790505f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Action potentials</topic><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Cell culture techniques</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Cultured cells</topic><topic>Electric Conductivity</topic><topic>Electric current</topic><topic>Endorphins - pharmacology</topic><topic>Enkephalins - pharmacology</topic><topic>Ganglia, Spinal - drug effects</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Materials</topic><topic>Membrane Potentials - drug effects</topic><topic>Nerve Endings - drug effects</topic><topic>Nerves</topic><topic>Neurons</topic><topic>Sensory neurons</topic><topic>Spinal cord</topic><topic>Substance P - metabolism</topic><topic>Synaptic Transmission - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mudge, Anne W.</creatorcontrib><creatorcontrib>Leeman, Susan E.</creatorcontrib><creatorcontrib>Fischbach, Gerald D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mudge, Anne W.</au><au>Leeman, Susan E.</au><au>Fischbach, Gerald D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enkephalin Inhibits Release of Substance P from Sensory Neurons in Culture and Decreases Action Potential Duration</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-01-01</date><risdate>1979</risdate><volume>76</volume><issue>1</issue><spage>526</spage><epage>530</epage><pages>526-530</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 μ M inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>218204</pmid><doi>10.1073/pnas.76.1.526</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-01, Vol.76 (1), p.526-530 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_69531 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Action potentials Action Potentials - drug effects Animals Cell culture techniques Cells, Cultured Chick Embryo Cultured cells Electric Conductivity Electric current Endorphins - pharmacology Enkephalins - pharmacology Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism Materials Membrane Potentials - drug effects Nerve Endings - drug effects Nerves Neurons Sensory neurons Spinal cord Substance P - metabolism Synaptic Transmission - drug effects |
| title | Enkephalin Inhibits Release of Substance P from Sensory Neurons in Culture and Decreases Action Potential Duration |
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