Evidence for Unaltered Structure and in vivo Assembly of Microtubules in Transformed Cells
By using immunoperoxidase cytochemistry at the light and electron microscopic level, microtubles were visualized in a number of ``normal'' nontumorigenic and transformed tumorigenic cell lines. A well-defined cytoplasmic microtubule complex exists in both normal and transformed interphase...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1978-03, Vol.75 (3), p.1339-1343 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | De Mey, J. Joniau, M. De Brabander, M. Moens, W. Geuens, G. |
| description | By using immunoperoxidase cytochemistry at the light and electron microscopic level, microtubles were visualized in a number of ``normal'' nontumorigenic and transformed tumorigenic cell lines. A well-defined cytoplasmic microtubule complex exists in both normal and transformed interphase cells. The distribution of this complex closely correlates with the cell shape and the degree of cell spreading. Our data support the idea that these properties determine the pattern of the cytoplasmic microtubule complex, rather than the reverse. Ultrastructural observations of immunoperoxidase-stained tumor cells showed characteristic microtubules in cells in which the microtubules were poorly resolved at the light microscopic level. The results suggest that microtubule assembly and structure are unaltered in transformed cells. However, this conclusion does not exclude the possibility that some of the microtubules' functions might be impaired in a yet-unknown way. |
| doi_str_mv | 10.1073/pnas.75.3.1339 |
| format | Article |
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However, this conclusion does not exclude the possibility that some of the microtubules' functions might be impaired in a yet-unknown way.</description><subject>Antibodies</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>Cells</subject><subject>Electron microscopy</subject><subject>Electrons</subject><subject>Fluorescent antibody techniques</subject><subject>HeLa cells</subject><subject>Immunoenzyme Techniques</subject><subject>Interphase</subject><subject>Microtubules</subject><subject>Microtubules - metabolism</subject><subject>Microtubules - ultrastructure</subject><subject>Neurons</subject><subject>Transformed cell line</subject><subject>Tubulin - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kTtP5DAUhS3Ea3i0FAgkV3QJdvwaFxRoxO4igSiAhsZyHHsJytiDnYzg36-jmR1BQ-XifOce33sAOMGoxEiQy4XXqRSsJCUmRG6BCUYSF5xKtA0mCFWimNKK7oODlN4QQpJN0R7YJVQyTifg5WbZNtYbC12I8NnrrrfRNvCxj4Pph2ih9g1sPVy2ywCvU7LzuvuEwcH71sTQD_XQ2TQCT1H7lIfMs3tmuy4dgR2nu2SP1-8heP518zT7U9w9_L6dXd8Vhggki1o3WGDOHSeVdBpz6SjlVhNqqCSSaENIZWRDde2oY0Jjx9iUkkobiYyckkNwtZq7GOocbqzvo-7UIrZzHT9V0K36rvj2Vf0NS0Uxppxn_8XaH8P7YFOv5m0yeQPtbRiSEhRhhtkYVK7AvHhK0bpNBkZq7EKNXSjBFFFjF9lw9vVnG3x1_Cyfr-XR9l_8ar_4SVdu6HJbH30GT1fgW-pD3JBcSMnIPzMpp28</recordid><startdate>19780301</startdate><enddate>19780301</enddate><creator>De Mey, J.</creator><creator>Joniau, M.</creator><creator>De Brabander, M.</creator><creator>Moens, W.</creator><creator>Geuens, G.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19780301</creationdate><title>Evidence for Unaltered Structure and in vivo Assembly of Microtubules in Transformed Cells</title><author>De Mey, J. ; Joniau, M. ; De Brabander, M. ; Moens, W. ; Geuens, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3709-bad17166f6329fa169f446ea34c49393ac332c9d4abf4f57a1f558432ac90c983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Antibodies</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>Cells</topic><topic>Electron microscopy</topic><topic>Electrons</topic><topic>Fluorescent antibody techniques</topic><topic>HeLa cells</topic><topic>Immunoenzyme Techniques</topic><topic>Interphase</topic><topic>Microtubules</topic><topic>Microtubules - metabolism</topic><topic>Microtubules - ultrastructure</topic><topic>Neurons</topic><topic>Transformed cell line</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Mey, J.</creatorcontrib><creatorcontrib>Joniau, M.</creatorcontrib><creatorcontrib>De Brabander, M.</creatorcontrib><creatorcontrib>Moens, W.</creatorcontrib><creatorcontrib>Geuens, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Mey, J.</au><au>Joniau, M.</au><au>De Brabander, M.</au><au>Moens, W.</au><au>Geuens, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Unaltered Structure and in vivo Assembly of Microtubules in Transformed Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1978-03-01</date><risdate>1978</risdate><volume>75</volume><issue>3</issue><spage>1339</spage><epage>1343</epage><pages>1339-1343</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>By using immunoperoxidase cytochemistry at the light and electron microscopic level, microtubles were visualized in a number of ``normal'' nontumorigenic and transformed tumorigenic cell lines. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1978-03, Vol.75 (3), p.1339-1343 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_67995 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antibodies Cell Line Cell lines Cell Transformation, Neoplastic - pathology Cells Electron microscopy Electrons Fluorescent antibody techniques HeLa cells Immunoenzyme Techniques Interphase Microtubules Microtubules - metabolism Microtubules - ultrastructure Neurons Transformed cell line Tubulin - metabolism |
| title | Evidence for Unaltered Structure and in vivo Assembly of Microtubules in Transformed Cells |
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