Changes in Membrane Microviscosity Associated with Phagocytosis: Effects of Colchicine
The effects of phagocytosis on plasma membrane microviscosity were studied by fluorescence depolarization techniques. It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the beha...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1977-03, Vol.74 (3), p.1072-1076 |
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| container_end_page | 1076 |
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| container_issue | 3 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 74 |
| creator | Berlin, Richard D. Fera, Judith P. |
| description | The effects of phagocytosis on plasma membrane microviscosity were studied by fluorescence depolarization techniques. It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the behavior of probes in isolated membranes. Phagocytosis of oil emulsions or polystyrene beads by rabbit polymorphonuclear leukocytes induces a marked decrease in plasma membrane microviscosity that parallels the extent of phagocytosis. Liposomes made from extracts of membrane lipid show qualitatively the same changes, indicating that the alteration of microviscosity results at least in part from changes in lipid composition. The decrease in microviscosity is abolished when colchicine is present during phagocytosis. Addition of colchicine to membranes previously isolated from control or phagocytic cells has no effect on their microviscosity. The results suggest that phagocytosis is accompanied by a microtubule-dependent reorganization of membrane lipids. |
| doi_str_mv | 10.1073/pnas.74.3.1072 |
| format | Article |
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It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the behavior of probes in isolated membranes. Phagocytosis of oil emulsions or polystyrene beads by rabbit polymorphonuclear leukocytes induces a marked decrease in plasma membrane microviscosity that parallels the extent of phagocytosis. Liposomes made from extracts of membrane lipid show qualitatively the same changes, indicating that the alteration of microviscosity results at least in part from changes in lipid composition. The decrease in microviscosity is abolished when colchicine is present during phagocytosis. Addition of colchicine to membranes previously isolated from control or phagocytic cells has no effect on their microviscosity. The results suggest that phagocytosis is accompanied by a microtubule-dependent reorganization of membrane lipids.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.74.3.1072</identifier><identifier>PMID: 265552</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Cell Membrane - physiology ; Cell membranes ; Colchicine - pharmacology ; Depolarization ; Emulsions ; Fibroblasts ; Fluorescence ; Fluorescent Dyes ; Lipids ; Liposomes ; Microscopy, Fluorescence ; Microtubules - physiology ; Neutrophils ; P branes ; Phagocytes ; Phagocytosis ; Phagocytosis - drug effects ; Rabbits ; Viscosity</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1977-03, Vol.74 (3), p.1072-1076</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-838c527c43f764e222bea137c6179fc7109d2d1cc2e3ff5a85c1bf96ca4898833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/74/3.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/66343$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/66343$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27922,27923,53789,53791,58015,58248</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/265552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berlin, Richard D.</creatorcontrib><creatorcontrib>Fera, Judith P.</creatorcontrib><title>Changes in Membrane Microviscosity Associated with Phagocytosis: Effects of Colchicine</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The effects of phagocytosis on plasma membrane microviscosity were studied by fluorescence depolarization techniques. It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the behavior of probes in isolated membranes. Phagocytosis of oil emulsions or polystyrene beads by rabbit polymorphonuclear leukocytes induces a marked decrease in plasma membrane microviscosity that parallels the extent of phagocytosis. Liposomes made from extracts of membrane lipid show qualitatively the same changes, indicating that the alteration of microviscosity results at least in part from changes in lipid composition. The decrease in microviscosity is abolished when colchicine is present during phagocytosis. Addition of colchicine to membranes previously isolated from control or phagocytic cells has no effect on their microviscosity. The results suggest that phagocytosis is accompanied by a microtubule-dependent reorganization of membrane lipids.</description><subject>Animals</subject><subject>Cell Membrane - physiology</subject><subject>Cell membranes</subject><subject>Colchicine - pharmacology</subject><subject>Depolarization</subject><subject>Emulsions</subject><subject>Fibroblasts</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Lipids</subject><subject>Liposomes</subject><subject>Microscopy, Fluorescence</subject><subject>Microtubules - physiology</subject><subject>Neutrophils</subject><subject>P branes</subject><subject>Phagocytes</subject><subject>Phagocytosis</subject><subject>Phagocytosis - drug effects</subject><subject>Rabbits</subject><subject>Viscosity</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1PGzEQtVCBpsCVA2oln7ht8Od6F4kDiqBFAsGh5Wo5EztrtFmHtUPJv8er0IheOI1G7715M28QOqZkTIniZ8vOxLESYz60bAeNKKlpUYqafEEjQpgqKsHEV_QtxidCSC0rso_2WCmlZCP0OGlMN7cR-w7f2cW0N53Fdx768OIjhOjTGl_GGMCbZGf4r08NfmjMPMA6ZTSe4yvnLKSIg8OT0ELjwXf2EO0600Z79F4P0J_rq9-TX8Xt_c-byeVtAULKVFS8AskUCO5UKSxjbGoN5QpKqmoHKp8yYzMKwCx3TppKAp26ugQjqrqqOD9AF5u5y9V0YWdgu9SbVi97vzD9Wgfj9f9I5xs9Dy9acCJrmfWn7_o-PK9sTHqRz7Ztm2MIq6jzgrImbDAab4g5mRh767YelOjhD3r4g1ZC86FlWfD942Zb-ib4D8aD7B-4lWu3attkX1Mm_viMmPGTDf4UU-i3hLLkgvM30BimLw</recordid><startdate>19770301</startdate><enddate>19770301</enddate><creator>Berlin, Richard D.</creator><creator>Fera, Judith P.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19770301</creationdate><title>Changes in Membrane Microviscosity Associated with Phagocytosis: Effects of Colchicine</title><author>Berlin, Richard D. ; Fera, Judith P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-838c527c43f764e222bea137c6179fc7109d2d1cc2e3ff5a85c1bf96ca4898833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Cell Membrane - physiology</topic><topic>Cell membranes</topic><topic>Colchicine - pharmacology</topic><topic>Depolarization</topic><topic>Emulsions</topic><topic>Fibroblasts</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes</topic><topic>Lipids</topic><topic>Liposomes</topic><topic>Microscopy, Fluorescence</topic><topic>Microtubules - physiology</topic><topic>Neutrophils</topic><topic>P branes</topic><topic>Phagocytes</topic><topic>Phagocytosis</topic><topic>Phagocytosis - drug effects</topic><topic>Rabbits</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berlin, Richard D.</creatorcontrib><creatorcontrib>Fera, Judith P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berlin, Richard D.</au><au>Fera, Judith P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in Membrane Microviscosity Associated with Phagocytosis: Effects of Colchicine</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1977-03-01</date><risdate>1977</risdate><volume>74</volume><issue>3</issue><spage>1072</spage><epage>1076</epage><pages>1072-1076</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The effects of phagocytosis on plasma membrane microviscosity were studied by fluorescence depolarization techniques. It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the behavior of probes in isolated membranes. Phagocytosis of oil emulsions or polystyrene beads by rabbit polymorphonuclear leukocytes induces a marked decrease in plasma membrane microviscosity that parallels the extent of phagocytosis. Liposomes made from extracts of membrane lipid show qualitatively the same changes, indicating that the alteration of microviscosity results at least in part from changes in lipid composition. The decrease in microviscosity is abolished when colchicine is present during phagocytosis. Addition of colchicine to membranes previously isolated from control or phagocytic cells has no effect on their microviscosity. The results suggest that phagocytosis is accompanied by a microtubule-dependent reorganization of membrane lipids.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>265552</pmid><doi>10.1073/pnas.74.3.1072</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1977-03, Vol.74 (3), p.1072-1076 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Cell Membrane - physiology Cell membranes Colchicine - pharmacology Depolarization Emulsions Fibroblasts Fluorescence Fluorescent Dyes Lipids Liposomes Microscopy, Fluorescence Microtubules - physiology Neutrophils P branes Phagocytes Phagocytosis Phagocytosis - drug effects Rabbits Viscosity |
| title | Changes in Membrane Microviscosity Associated with Phagocytosis: Effects of Colchicine |
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