Partial Amino-Acid Sequence of NAD-Specific Glutamate Dehydrogenase of Neurospora crassa

Parts of the primary structure of the NAD-specific glutamate dehydrogenase [L-glutamate:NAD oxido-reductase (deaminating), EC 1.4.1.2] from Neurospora crassa are presented. Segments of the sequence representing 886 unique amino-acid residues have been determined; the largest contains 267 residues. There are only short regions of possible homology between this enzyme and the glutamate dehydrogenases of bovine liver or the NADP-specific enzyme of Neurospora. The large size of the subunit (116,000 molecular weight) of the NAD-specific glutamate dehydrogenase is unusual when compared to other known dehydrogenases.