Soluble Complexes between Steroid Hormones and Target-Tissue Receptors Bind Specifically to Target-Tissue Chromatin
Cytoplasmic fractions containing steroid hormone receptor were prepared from rat prostate and uterine tissues, incubated first with [3H]dihydrotestosterone or [3H]estradiol, and then with their respective target and non-target tissue chromatins. Only prostate and testis chromatin bound the dihydrote...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1971-07, Vol.68 (7), p.1479-1482 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Steggles, A. W. Spelsberg, T. C. Glasser, S. R. O'Malley, B. W. |
| description | Cytoplasmic fractions containing steroid hormone receptor were prepared from rat prostate and uterine tissues, incubated first with [3H]dihydrotestosterone or [3H]estradiol, and then with their respective target and non-target tissue chromatins. Only prostate and testis chromatin bound the dihydrotestosterone-receptor complex from prostate cytosol extensively. Similarly, uterine chromatin bound more estradiol-receptor complex from uterus than did liver, spleen, or lung chromatin. Complexes between dihydrotestosterone or estradiol with cytosols prepared from liver and spleen bound less extensively, and similarly, to all chromatins. Analogous results are described for the [3H]progesterone-receptor complex from chick oviduct cytosol binding to oviduct chromatin. These studies suggest that the chromatin of all steroid hormone target tissues may contain ``acceptor sites'' for their respective hormone-receptor complexes, and are thus programmed to receive the complex as it is transferred into the nucleus from the cytoplasm of the cell. |
| doi_str_mv | 10.1073/pnas.68.7.1479 |
| format | Article |
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W.</creatorcontrib><creatorcontrib>Spelsberg, T. C.</creatorcontrib><creatorcontrib>Glasser, S. R.</creatorcontrib><creatorcontrib>O'Malley, B. W.</creatorcontrib><title>Soluble Complexes between Steroid Hormones and Target-Tissue Receptors Bind Specifically to Target-Tissue Chromatin</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cytoplasmic fractions containing steroid hormone receptor were prepared from rat prostate and uterine tissues, incubated first with [3H]dihydrotestosterone or [3H]estradiol, and then with their respective target and non-target tissue chromatins. Only prostate and testis chromatin bound the dihydrotestosterone-receptor complex from prostate cytosol extensively. Similarly, uterine chromatin bound more estradiol-receptor complex from uterus than did liver, spleen, or lung chromatin. Complexes between dihydrotestosterone or estradiol with cytosols prepared from liver and spleen bound less extensively, and similarly, to all chromatins. Analogous results are described for the [3H]progesterone-receptor complex from chick oviduct cytosol binding to oviduct chromatin. These studies suggest that the chromatin of all steroid hormone target tissues may contain ``acceptor sites'' for their respective hormone-receptor complexes, and are thus programmed to receive the complex as it is transferred into the nucleus from the cytoplasm of the cell.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological Sciences: Biochemistry</subject><subject>Carbon Isotopes</subject><subject>Castration</subject><subject>Centrifugation, Density Gradient</subject><subject>Chickens</subject><subject>Chromatin</subject><subject>Chromosomes - analysis</subject><subject>Chromosomes - metabolism</subject><subject>Cytosol</subject><subject>Dihydrotestosterone - metabolism</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - biosynthesis</subject><subject>Estradiol - metabolism</subject><subject>Female</subject><subject>Histones - analysis</subject><subject>Kidney - metabolism</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Lung - metabolism</subject><subject>Lungs</subject><subject>Male</subject><subject>Nucleic Acids - metabolism</subject><subject>Oviducts</subject><subject>Oviducts - metabolism</subject><subject>Progesterone - metabolism</subject><subject>Prostate</subject><subject>Prostate - cytology</subject><subject>Prostate - metabolism</subject><subject>Rats</subject><subject>Receptors</subject><subject>Receptors, Drug</subject><subject>Spleen</subject><subject>Spleen - metabolism</subject><subject>Steroids</subject><subject>Testis - metabolism</subject><subject>Tritium</subject><subject>Uracil Nucleotides - metabolism</subject><subject>Uterus - cytology</subject><subject>Uterus - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb9v1DAcRy0EKkfLyoCElKlbUv9KbA8McAKKVAmpd8yW43zTunLiYDvQ_vfkdEe5MjB5eO_Zlj4IvSG4Iliwi2k0qWpkJSrChXqGVgQrUjZc4edohTEVpeSUv0SvUrrDGKta4hN0UlPJFJMrlDbBz62HYh2GycM9pKKF_AtgLDYZYnBdcRniEMYFmLErtibeQC63LqUZimuwMOUQU_HRLXAzgXW9s8b7hyKHf-T1bQyDyW48Qy964xO8Ppyn6PvnT9v1ZXn17cvX9Yer0vK6yaVpKbWytsrWSmBTW8ukaKkh0FhMmWxoVyuwom47LnrM2460PWDRcWUXTbBT9H5_7zS3A3QWxhyN11N0g4kPOhinn5LR3eqb8FMzqSilS39-6GP4MUPKenDJgvdmhDAnLbhiiki8iNVetDGkFKF_fINgvVtJ71bSjdRC71ZagnfHP3vUD7Mc8V33hx735__jup-9z3CfF_HtXrxLy0x_TSwoY78BZ5eyfQ</recordid><startdate>19710701</startdate><enddate>19710701</enddate><creator>Steggles, A. W.</creator><creator>Spelsberg, T. C.</creator><creator>Glasser, S. R.</creator><creator>O'Malley, B. W.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19710701</creationdate><title>Soluble Complexes between Steroid Hormones and Target-Tissue Receptors Bind Specifically to Target-Tissue Chromatin</title><author>Steggles, A. W. ; Spelsberg, T. C. ; Glasser, S. R. ; O'Malley, B. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ab22c85c9c5970a5cc387b2a1e6c023862d59ec75bd47f04bd1bfe07d49cb2a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological Sciences: Biochemistry</topic><topic>Carbon Isotopes</topic><topic>Castration</topic><topic>Centrifugation, Density Gradient</topic><topic>Chickens</topic><topic>Chromatin</topic><topic>Chromosomes - analysis</topic><topic>Chromosomes - metabolism</topic><topic>Cytosol</topic><topic>Dihydrotestosterone - metabolism</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - biosynthesis</topic><topic>Estradiol - metabolism</topic><topic>Female</topic><topic>Histones - analysis</topic><topic>Kidney - metabolism</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Lung - metabolism</topic><topic>Lungs</topic><topic>Male</topic><topic>Nucleic Acids - metabolism</topic><topic>Oviducts</topic><topic>Oviducts - metabolism</topic><topic>Progesterone - metabolism</topic><topic>Prostate</topic><topic>Prostate - cytology</topic><topic>Prostate - metabolism</topic><topic>Rats</topic><topic>Receptors</topic><topic>Receptors, Drug</topic><topic>Spleen</topic><topic>Spleen - metabolism</topic><topic>Steroids</topic><topic>Testis - metabolism</topic><topic>Tritium</topic><topic>Uracil Nucleotides - metabolism</topic><topic>Uterus - cytology</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steggles, A. W.</creatorcontrib><creatorcontrib>Spelsberg, T. C.</creatorcontrib><creatorcontrib>Glasser, S. R.</creatorcontrib><creatorcontrib>O'Malley, B. W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steggles, A. W.</au><au>Spelsberg, T. C.</au><au>Glasser, S. R.</au><au>O'Malley, B. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble Complexes between Steroid Hormones and Target-Tissue Receptors Bind Specifically to Target-Tissue Chromatin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1971-07-01</date><risdate>1971</risdate><volume>68</volume><issue>7</issue><spage>1479</spage><epage>1482</epage><pages>1479-1482</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cytoplasmic fractions containing steroid hormone receptor were prepared from rat prostate and uterine tissues, incubated first with [3H]dihydrotestosterone or [3H]estradiol, and then with their respective target and non-target tissue chromatins. Only prostate and testis chromatin bound the dihydrotestosterone-receptor complex from prostate cytosol extensively. Similarly, uterine chromatin bound more estradiol-receptor complex from uterus than did liver, spleen, or lung chromatin. Complexes between dihydrotestosterone or estradiol with cytosols prepared from liver and spleen bound less extensively, and similarly, to all chromatins. Analogous results are described for the [3H]progesterone-receptor complex from chick oviduct cytosol binding to oviduct chromatin. These studies suggest that the chromatin of all steroid hormone target tissues may contain ``acceptor sites'' for their respective hormone-receptor complexes, and are thus programmed to receive the complex as it is transferred into the nucleus from the cytoplasm of the cell.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>5283938</pmid><doi>10.1073/pnas.68.7.1479</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Binding Sites Biological Sciences: Biochemistry Carbon Isotopes Castration Centrifugation, Density Gradient Chickens Chromatin Chromosomes - analysis Chromosomes - metabolism Cytosol Dihydrotestosterone - metabolism DNA DNA - analysis DNA - biosynthesis Estradiol - metabolism Female Histones - analysis Kidney - metabolism Liver Liver - metabolism Lung - metabolism Lungs Male Nucleic Acids - metabolism Oviducts Oviducts - metabolism Progesterone - metabolism Prostate Prostate - cytology Prostate - metabolism Rats Receptors Receptors, Drug Spleen Spleen - metabolism Steroids Testis - metabolism Tritium Uracil Nucleotides - metabolism Uterus - cytology Uterus - metabolism |
| title | Soluble Complexes between Steroid Hormones and Target-Tissue Receptors Bind Specifically to Target-Tissue Chromatin |
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