Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling
The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1971-05, Vol.68 (5), p.873-876 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 68 |
| creator | Kanabus, Jan Cherry, Joe H. |
| description | The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well. Soybean hypocotyls appear to contain only the last two enzyme fractions. |
| doi_str_mv | 10.1073/pnas.68.5.873 |
| format | Article |
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One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well. Soybean hypocotyls appear to contain only the last two enzyme fractions.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.68.5.873</identifier><identifier>PMID: 5280524</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Biological Sciences: Biochemistry ; Chromatography ; Cotyledons ; Enzymes ; Fractionation ; Freon ; Glycine max ; Hydrocarbons, Halogenated ; Hydroxyapatites ; Hypocotyls ; Leucine - metabolism ; Ligases - analysis ; Ligases - isolation & purification ; Organ Specificity ; Potassium phosphates ; RNA, Transfer - metabolism ; Seeds - enzymology ; Soybeans ; Transfer RNA</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1971-05, Vol.68 (5), p.873-876</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3663-e9099390857d20b6ec4d4995ade1d50df0a4005bf8e5d0705d05bcd5f1b4683e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/68/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/60603$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/60603$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5280524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanabus, Jan</creatorcontrib><creatorcontrib>Cherry, Joe H.</creatorcontrib><title>Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well. Soybean hypocotyls appear to contain only the last two enzyme fractions.</description><subject>Amino acids</subject><subject>Biological Sciences: Biochemistry</subject><subject>Chromatography</subject><subject>Cotyledons</subject><subject>Enzymes</subject><subject>Fractionation</subject><subject>Freon</subject><subject>Glycine max</subject><subject>Hydrocarbons, Halogenated</subject><subject>Hydroxyapatites</subject><subject>Hypocotyls</subject><subject>Leucine - metabolism</subject><subject>Ligases - analysis</subject><subject>Ligases - isolation & purification</subject><subject>Organ Specificity</subject><subject>Potassium phosphates</subject><subject>RNA, Transfer - metabolism</subject><subject>Seeds - enzymology</subject><subject>Soybeans</subject><subject>Transfer RNA</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vEzEQhi0EKqFw5IJA2gvcNsyu1177wKGq-KiIqETgbHntcbqVYwfbi8i_Z6OEUi5cPIfnecejl5DnDSwb6OnbXdB5ycWSLUVPH5BFA7KpeSfhIVkAtH0turZ7TJ7kfAsAkgk4I2esFcDabkE-X-XodRljqKKrdKiu00aHer1DM7rRVCuczN7X5euXi2q9D-UGi85YuRS31TruB5wja0Trx7B5Sh457TM-O81z8v3D-2-Xn-rV9cery4tVbSjntEYJUlIJgvW2hYGj6WwnJdMWG8vAOtAdABucQGahh_lhg7HMNUPHBUV6Tt4d9-6mYYvWYChJe7VL41anvYp6VP-SMN6oTfypqJDA5Zx_c8qn-GPCXNR2zAa91wHjlJUA2UtKD2J9FE2KOSd0d380oA7lq0P5igvF1Fz-7L-6f9idfWp75i9P_BD7Q-_FX_8HKzd5X_BXmb0XR-82l5j-isCB0t8OOqES</recordid><startdate>19710501</startdate><enddate>19710501</enddate><creator>Kanabus, Jan</creator><creator>Cherry, Joe H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19710501</creationdate><title>Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling</title><author>Kanabus, Jan ; Cherry, Joe H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3663-e9099390857d20b6ec4d4995ade1d50df0a4005bf8e5d0705d05bcd5f1b4683e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Amino acids</topic><topic>Biological Sciences: Biochemistry</topic><topic>Chromatography</topic><topic>Cotyledons</topic><topic>Enzymes</topic><topic>Fractionation</topic><topic>Freon</topic><topic>Glycine max</topic><topic>Hydrocarbons, Halogenated</topic><topic>Hydroxyapatites</topic><topic>Hypocotyls</topic><topic>Leucine - metabolism</topic><topic>Ligases - analysis</topic><topic>Ligases - isolation & purification</topic><topic>Organ Specificity</topic><topic>Potassium phosphates</topic><topic>RNA, Transfer - metabolism</topic><topic>Seeds - enzymology</topic><topic>Soybeans</topic><topic>Transfer RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanabus, Jan</creatorcontrib><creatorcontrib>Cherry, Joe H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanabus, Jan</au><au>Cherry, Joe H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1971-05-01</date><risdate>1971</risdate><volume>68</volume><issue>5</issue><spage>873</spage><epage>876</epage><pages>873-876</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1971-05, Vol.68 (5), p.873-876 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Biological Sciences: Biochemistry Chromatography Cotyledons Enzymes Fractionation Freon Glycine max Hydrocarbons, Halogenated Hydroxyapatites Hypocotyls Leucine - metabolism Ligases - analysis Ligases - isolation & purification Organ Specificity Potassium phosphates RNA, Transfer - metabolism Seeds - enzymology Soybeans Transfer RNA |
| title | Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling |
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