An Escherichia coli Strain with All Chromosomal rRNA Operons Inactivated: Complete Exchange of rRNA Genes between Bacteria

Current global phylogenies are built predominantly on rRNA sequences. However, an experimental system for studying the evolution of rRNA is not readily available, mainly because the rRNA genes are highly repeated in most experimental organisms. We have constructed an Escherichia coli strain in which...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1999-03, Vol.96 (5), p.1971-1976
Hauptverfasser:	Asai, Tsuneaki, Zaporojets, Dmitry, Squires, Craig, Squires, Catherine L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Base Sequence Biochemistry Biological Sciences Chromosomes, Bacterial - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Genes Genes, Bacterial Hybridity Molecules Operator regions Operon Operons Plasmids Polymerase Chain Reaction Proteus vulgaris Restriction Mapping Ribonucleic acid Ribosomes RNA RNA, Bacterial - genetics RNA, Ribosomal - genetics RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics rRNA genes rRNA operon Saccharomyces cerevisiae Salmonella typhimurium Salmonella typhimurium - genetics Sequence Deletion Transfer RNA
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