A Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains
The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between pathogenic and nonpathogenic strains, we identified a chromosomal pathogenicity island (PAI) that is p...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-03, Vol.95 (6), p.3134-3139 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | David K. R. Karaolis Johnson, Judith A. Bailey, Camella C. Boedeker, Edgar C. Kaper, James B. Reeves, Peter R. |
| description | The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between pathogenic and nonpathogenic strains, we identified a chromosomal pathogenicity island (PAI) that is present in epidemic and pandemic strains but absent from nonpathogenic strains. Initially, two ToxR-regulated genes (aldA and tagA) were studied and were found to be associated with epidemic and pandemic strains but absent in nontoxigenic strains. The region containing aldA and tagA comprises 13 kb of previously unidentified DNA and is part of a PAI that contains a regulator of virulence genes (ToxT) and a gene cluster encoding an essential colonization factor and the cholera toxin phage receptor (toxin-coregulated pilus; TCP). The PAI is 39.5 kb in size, has low%G+C (35%), contains putative integrase and transposase genes, is flanked by att sites, and inserts near a 10Sa RNA gene (ssrA), suggesting it may be of bacteriophage origin. We found this PAI in two clinical non-O1/non-O139 cholera toxin-positive strains, suggesting that it can be transferred within V. cholerae. The sequence within this PAI includes an ORF with homology to a gene associated with the type IV pilus gene cluster of enteropathogenic Escherichia coli, a transposase from Vibrio anguillarum, and several ORFs with no known homology. As the PAI contains the CTXΦ receptor, it may represent the initial genetic factor required for the emergence of epidemic and pandemic cholera. We propose to call this island VPI (V. cholerae pathogenicity island). |
| doi_str_mv | 10.1073/pnas.95.6.3134 |
| format | Article |
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R. Karaolis ; Johnson, Judith A. ; Bailey, Camella C. ; Boedeker, Edgar C. ; Kaper, James B. ; Reeves, Peter R.</creator><creatorcontrib>David K. R. Karaolis ; Johnson, Judith A. ; Bailey, Camella C. ; Boedeker, Edgar C. ; Kaper, James B. ; Reeves, Peter R.</creatorcontrib><description>The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between pathogenic and nonpathogenic strains, we identified a chromosomal pathogenicity island (PAI) that is present in epidemic and pandemic strains but absent from nonpathogenic strains. Initially, two ToxR-regulated genes (aldA and tagA) were studied and were found to be associated with epidemic and pandemic strains but absent in nontoxigenic strains. The region containing aldA and tagA comprises 13 kb of previously unidentified DNA and is part of a PAI that contains a regulator of virulence genes (ToxT) and a gene cluster encoding an essential colonization factor and the cholera toxin phage receptor (toxin-coregulated pilus; TCP). The PAI is 39.5 kb in size, has low%G+C (35%), contains putative integrase and transposase genes, is flanked by att sites, and inserts near a 10Sa RNA gene (ssrA), suggesting it may be of bacteriophage origin. We found this PAI in two clinical non-O1/non-O139 cholera toxin-positive strains, suggesting that it can be transferred within V. cholerae. The sequence within this PAI includes an ORF with homology to a gene associated with the type IV pilus gene cluster of enteropathogenic Escherichia coli, a transposase from Vibrio anguillarum, and several ORFs with no known homology. As the PAI contains the CTXΦ receptor, it may represent the initial genetic factor required for the emergence of epidemic and pandemic cholera. We propose to call this island VPI (V. cholerae pathogenicity island).</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.95.6.3134</identifier><identifier>PMID: 9501228</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Aldehyde Dehydrogenase - genetics ; Bacteria ; Bacterial Proteins - genetics ; Base Composition ; Biological Sciences ; Cholera ; Cholera - epidemiology ; Cholera - etiology ; Cosmids ; Disease Outbreaks ; DNA ; DNA Transposable Elements ; Epidemics ; Epidemiology ; Fimbriae, Bacterial - genetics ; Genes ; Genes, Bacterial ; Genetic transposition ; Humans ; Lipoproteins - genetics ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Pandemics ; Polymerase chain reaction ; Sequence Analysis, DNA ; Transcription Factors - genetics ; United States ; Vibrio cholerae - classification ; Vibrio cholerae - genetics ; Vibrio cholerae - pathogenicity ; Virulence ; Virulence - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1998-03, Vol.95 (6), p.3134-3139</ispartof><rights>Copyright 1993-1998 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Mar 17, 1998</rights><rights>Copyright © 1998, The National Academy of Sciences 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-cec3a02b832a92641b28010f64380ea878b2b92ca3ef5b34a648e340de3136cb3</citedby><cites>FETCH-LOGICAL-c513t-cec3a02b832a92641b28010f64380ea878b2b92ca3ef5b34a648e340de3136cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/95/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/44810$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/44810$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9501228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>David K. R. Karaolis</creatorcontrib><creatorcontrib>Johnson, Judith A.</creatorcontrib><creatorcontrib>Bailey, Camella C.</creatorcontrib><creatorcontrib>Boedeker, Edgar C.</creatorcontrib><creatorcontrib>Kaper, James B.</creatorcontrib><creatorcontrib>Reeves, Peter R.</creatorcontrib><title>A Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between pathogenic and nonpathogenic strains, we identified a chromosomal pathogenicity island (PAI) that is present in epidemic and pandemic strains but absent from nonpathogenic strains. Initially, two ToxR-regulated genes (aldA and tagA) were studied and were found to be associated with epidemic and pandemic strains but absent in nontoxigenic strains. The region containing aldA and tagA comprises 13 kb of previously unidentified DNA and is part of a PAI that contains a regulator of virulence genes (ToxT) and a gene cluster encoding an essential colonization factor and the cholera toxin phage receptor (toxin-coregulated pilus; TCP). The PAI is 39.5 kb in size, has low%G+C (35%), contains putative integrase and transposase genes, is flanked by att sites, and inserts near a 10Sa RNA gene (ssrA), suggesting it may be of bacteriophage origin. We found this PAI in two clinical non-O1/non-O139 cholera toxin-positive strains, suggesting that it can be transferred within V. cholerae. The sequence within this PAI includes an ORF with homology to a gene associated with the type IV pilus gene cluster of enteropathogenic Escherichia coli, a transposase from Vibrio anguillarum, and several ORFs with no known homology. As the PAI contains the CTXΦ receptor, it may represent the initial genetic factor required for the emergence of epidemic and pandemic cholera. 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Karaolis</creator><creator>Johnson, Judith A.</creator><creator>Bailey, Camella C.</creator><creator>Boedeker, Edgar C.</creator><creator>Kaper, James B.</creator><creator>Reeves, Peter R.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980317</creationdate><title>A Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains</title><author>David K. 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Karaolis</creatorcontrib><creatorcontrib>Johnson, Judith A.</creatorcontrib><creatorcontrib>Bailey, Camella C.</creatorcontrib><creatorcontrib>Boedeker, Edgar C.</creatorcontrib><creatorcontrib>Kaper, James B.</creatorcontrib><creatorcontrib>Reeves, Peter R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>David K. R. Karaolis</au><au>Johnson, Judith A.</au><au>Bailey, Camella C.</au><au>Boedeker, Edgar C.</au><au>Kaper, James B.</au><au>Reeves, Peter R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1998-03-17</date><risdate>1998</risdate><volume>95</volume><issue>6</issue><spage>3134</spage><epage>3139</epage><pages>3134-3139</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between pathogenic and nonpathogenic strains, we identified a chromosomal pathogenicity island (PAI) that is present in epidemic and pandemic strains but absent from nonpathogenic strains. Initially, two ToxR-regulated genes (aldA and tagA) were studied and were found to be associated with epidemic and pandemic strains but absent in nontoxigenic strains. The region containing aldA and tagA comprises 13 kb of previously unidentified DNA and is part of a PAI that contains a regulator of virulence genes (ToxT) and a gene cluster encoding an essential colonization factor and the cholera toxin phage receptor (toxin-coregulated pilus; TCP). The PAI is 39.5 kb in size, has low%G+C (35%), contains putative integrase and transposase genes, is flanked by att sites, and inserts near a 10Sa RNA gene (ssrA), suggesting it may be of bacteriophage origin. We found this PAI in two clinical non-O1/non-O139 cholera toxin-positive strains, suggesting that it can be transferred within V. cholerae. The sequence within this PAI includes an ORF with homology to a gene associated with the type IV pilus gene cluster of enteropathogenic Escherichia coli, a transposase from Vibrio anguillarum, and several ORFs with no known homology. As the PAI contains the CTXΦ receptor, it may represent the initial genetic factor required for the emergence of epidemic and pandemic cholera. We propose to call this island VPI (V. cholerae pathogenicity island).</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>9501228</pmid><doi>10.1073/pnas.95.6.3134</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Aldehyde Dehydrogenase - genetics Bacteria Bacterial Proteins - genetics Base Composition Biological Sciences Cholera Cholera - epidemiology Cholera - etiology Cosmids Disease Outbreaks DNA DNA Transposable Elements Epidemics Epidemiology Fimbriae, Bacterial - genetics Genes Genes, Bacterial Genetic transposition Humans Lipoproteins - genetics Microbiology Molecular Sequence Data Multigene Family Pandemics Polymerase chain reaction Sequence Analysis, DNA Transcription Factors - genetics United States Vibrio cholerae - classification Vibrio cholerae - genetics Vibrio cholerae - pathogenicity Virulence Virulence - genetics |
| title | A Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains |
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