Measurement of Cell Proliferation by Labeling of DNA with Stable Isotope-Labeled Glucose: Studies in vitro, in Animals, and in Humans

A method for measuring DNA synthesis and, thus, cell proliferation, in vivo is presented. The technique consists of administering [6,6-2H2]Glc or [U-13C]Glc, isolating genomic DNA, hydrolyzing enzymatically to free deoxyribonucleosides, and derivatizing for GC-MS analysis of dA or dG isotopic enrich...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-01, Vol.95 (2), p.708-713
Hauptverfasser:	Macallan, Derek C., Fullerton, Catherine A., Neese, Richard A., Haddock, Katherine, Park, Sunny S., Hellerstein, Marc K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals B lymphocytes Biological Sciences Cell Division Cell growth Cell lines Cellular biology Cytological Techniques Deoxyribonucleic acid DNA DNA - analysis DNA - biosynthesis DNA Replication Glucose Hep G2 cells Hepatocytes Humans Isotope Labeling Isotopes Isotopic labeling Lymphocytes Radioisotopes Stem cells T lymphocytes Weights & measures
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