Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm
A cell-free enzyme system capable of metabolizing abscisic acid has been obtained from Eastern Wild Cucumber (Echinocystis lobata Michx.) liquid endosperm. The reaction products were determined to be phaseic acid (PA) and dihydrophaseic acid (DPA) by co-chromatography on thin layer chromatograms as...
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Veröffentlicht in: | Plant physiology (Bethesda) 1976-01, Vol.58 (6), p.790-795 |
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creator | Gillard D.F Walton D.C |
description | A cell-free enzyme system capable of metabolizing abscisic acid has been obtained from Eastern Wild Cucumber (Echinocystis lobata Michx.) liquid endosperm. The reaction products were determined to be phaseic acid (PA) and dihydrophaseic acid (DPA) by co-chromatography on thin layer chromatograms as the free acids, methyl esters, and their respective oxidation or reduction products. The crude enzyme preparation was separated by centrifugation into a particulate abscisic acid (ABA)-hydroxylating activity and a soluble PA-reducing activity. The particulate ABA-hydroxylating enzyme showed a requirement for O2 and NADPH, inhibition by CO, and high substrate specificity for (+)-ABA. Acetylation of short term incubation mixtures gave evidence for the presence of 6′-hydroxymethyl-ABA as an intermediate in PA formation. Determinations of endogenous ABA and DPA concentrations suggest that the ABA-hydroxylating and PA-reducing enzymes are extensively metabolizing ABA in the intact E. lobata seed. |
doi_str_mv | 10.1104/pp.58.6.790 |
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The reaction products were determined to be phaseic acid (PA) and dihydrophaseic acid (DPA) by co-chromatography on thin layer chromatograms as the free acids, methyl esters, and their respective oxidation or reduction products. The crude enzyme preparation was separated by centrifugation into a particulate abscisic acid (ABA)-hydroxylating activity and a soluble PA-reducing activity. The particulate ABA-hydroxylating enzyme showed a requirement for O2 and NADPH, inhibition by CO, and high substrate specificity for (+)-ABA. Acetylation of short term incubation mixtures gave evidence for the presence of 6′-hydroxymethyl-ABA as an intermediate in PA formation. Determinations of endogenous ABA and DPA concentrations suggest that the ABA-hydroxylating and PA-reducing enzymes are extensively metabolizing ABA in the intact E. lobata seed.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.58.6.790</identifier><language>eng</language><publisher>American Society of Plant Physiologists</publisher><subject>Acetylation ; Cellular metabolism ; Centrifugation ; Endosperm ; Enzyme activity ; Enzyme preparations ; Enzymes ; Esters ; Liquids ; Particulate matter</subject><ispartof>Plant physiology (Bethesda), 1976-01, Vol.58 (6), p.790-795</ispartof><rights>Copyright 1976 The American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4264650$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4264650$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids></links><search><creatorcontrib>Gillard D.F</creatorcontrib><creatorcontrib>Walton D.C</creatorcontrib><title>Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm</title><title>Plant physiology (Bethesda)</title><description>A cell-free enzyme system capable of metabolizing abscisic acid has been obtained from Eastern Wild Cucumber (Echinocystis lobata Michx.) liquid endosperm. The reaction products were determined to be phaseic acid (PA) and dihydrophaseic acid (DPA) by co-chromatography on thin layer chromatograms as the free acids, methyl esters, and their respective oxidation or reduction products. The crude enzyme preparation was separated by centrifugation into a particulate abscisic acid (ABA)-hydroxylating activity and a soluble PA-reducing activity. The particulate ABA-hydroxylating enzyme showed a requirement for O2 and NADPH, inhibition by CO, and high substrate specificity for (+)-ABA. Acetylation of short term incubation mixtures gave evidence for the presence of 6′-hydroxymethyl-ABA as an intermediate in PA formation. Determinations of endogenous ABA and DPA concentrations suggest that the ABA-hydroxylating and PA-reducing enzymes are extensively metabolizing ABA in the intact E. lobata seed.</description><subject>Acetylation</subject><subject>Cellular metabolism</subject><subject>Centrifugation</subject><subject>Endosperm</subject><subject>Enzyme activity</subject><subject>Enzyme preparations</subject><subject>Enzymes</subject><subject>Esters</subject><subject>Liquids</subject><subject>Particulate matter</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><recordid>eNotzE9LwzAcxvEgCtbpyatI3kBr0vyaP8cx5hQGHnRHKUmaaEbb1KQe9u4tzNPzwBc-CN1TUlFK4GmaqkZWvBKKXKCCNqwu6wbkJSoIWT6RUl2jm5yPhBDKKBToc22yDTlYrG3o8OBmbWIf8oDNCWtsXd-XPjmHp-QmnfQc4oh9igPe2u8wRnvKc8i4j0bPGvfh53dR3NjFPLk03KIrr_vs7v53hQ7P24_NS7l_271u1vvS01rNJa877wwIR1UnnGXKaidrDVZQ5S1rCBfMM-XAKE4BRGOUl9ISMIKDBcZW6OHsHvMcUzulMOh0aqHmwBuy5Mdz9jq2-iuF3B7eqRKCUJBswf8A2RBb8Q</recordid><startdate>19760101</startdate><enddate>19760101</enddate><creator>Gillard D.F</creator><creator>Walton D.C</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope></search><sort><creationdate>19760101</creationdate><title>Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm</title><author>Gillard D.F ; Walton D.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f129t-62dfeb47e19d7ec39cae82a4c719fc350673f39e4b9614475b9f88c04b764c433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Acetylation</topic><topic>Cellular metabolism</topic><topic>Centrifugation</topic><topic>Endosperm</topic><topic>Enzyme activity</topic><topic>Enzyme preparations</topic><topic>Enzymes</topic><topic>Esters</topic><topic>Liquids</topic><topic>Particulate matter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gillard D.F</creatorcontrib><creatorcontrib>Walton D.C</creatorcontrib><collection>AGRIS</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gillard D.F</au><au>Walton D.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>1976-01-01</date><risdate>1976</risdate><volume>58</volume><issue>6</issue><spage>790</spage><epage>795</epage><pages>790-795</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>A cell-free enzyme system capable of metabolizing abscisic acid has been obtained from Eastern Wild Cucumber (Echinocystis lobata Michx.) liquid endosperm. The reaction products were determined to be phaseic acid (PA) and dihydrophaseic acid (DPA) by co-chromatography on thin layer chromatograms as the free acids, methyl esters, and their respective oxidation or reduction products. The crude enzyme preparation was separated by centrifugation into a particulate abscisic acid (ABA)-hydroxylating activity and a soluble PA-reducing activity. The particulate ABA-hydroxylating enzyme showed a requirement for O2 and NADPH, inhibition by CO, and high substrate specificity for (+)-ABA. Acetylation of short term incubation mixtures gave evidence for the presence of 6′-hydroxymethyl-ABA as an intermediate in PA formation. Determinations of endogenous ABA and DPA concentrations suggest that the ABA-hydroxylating and PA-reducing enzymes are extensively metabolizing ABA in the intact E. lobata seed.</abstract><pub>American Society of Plant Physiologists</pub><doi>10.1104/pp.58.6.790</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Acetylation Cellular metabolism Centrifugation Endosperm Enzyme activity Enzyme preparations Enzymes Esters Liquids Particulate matter |
title | Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm |
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