Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion

Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology (Bethesda) 1970-09, Vol.46 (3), p.363-366
Hauptverfasser:	Doerschug, M.R, Chrispeels, M.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Azides botany Cell walls Cyanides Cytoplasm Exocrine cells Plant Science and Plant Products Plants Protein secretion Protein synthesis Proteins Secretion
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	366
container_issue	3
container_start_page	363
container_title	Plant physiology (Bethesda)
container_volume	46
creator	Doerschug, M.R Chrispeels, M.J
description	Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.
format	Article
fullrecord	<record><control><sourceid>jstor_fao_a</sourceid><recordid>TN_cdi_jstor_primary_4262179</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4262179</jstor_id><sourcerecordid>4262179</sourcerecordid><originalsourceid>FETCH-LOGICAL-f459-5b28e2cc14050882c36caebe067e90d3a202efc6997388c3d098cfbd7a9a93a93</originalsourceid><addsrcrecordid>eNpFUG1LwzAYDKLgnP4DwfyBjrw0bfJRhi-FgR82P5c0fbJltMlMMrD_3soE4eAOjrvn4a7QggrOCiZKeY0WhMyaSKlu0V1KR0II5bRcoGk7-XyA5BLWvscJTITsgsfB4sPUx_A9nWIYnIfCBJ-1887v8ahNDGMYwJwHSNh5bHSMIacVbppmhUfIuptTBkf4OrsII_icsA3x_8I9urF6SPDwx0u0e33Zrd-Lzcdbs37eFLYUqhAdk8CMoSUR8_vM8Mpo6IBUNSjSc80IA2sqpWoupeE9UdLYrq-10orPWKLHS-0x5RDbU3SjjlNbsorR-td-uthWh1bvo0vt55bN4xBKK8UE4z-oUGMX</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>JSTOR Archive Collection A-Z Listing</source><source>Alma/SFX Local Collection</source><creator>Doerschug, M.R ; Chrispeels, M.J</creator><creatorcontrib>Doerschug, M.R ; Chrispeels, M.J</creatorcontrib><description>Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><language>eng</language><publisher>American Society of Plant Physiologists</publisher><subject>Azides ; botany ; Cell walls ; Cyanides ; Cytoplasm ; Exocrine cells ; Plant Science and Plant Products ; Plants ; Protein secretion ; Protein synthesis ; Proteins ; Secretion</subject><ispartof>Plant physiology (Bethesda), 1970-09, Vol.46 (3), p.363-366</ispartof><rights>Copyright 1970 The American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4262179$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4262179$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,58017,58250</link.rule.ids></links><search><creatorcontrib>Doerschug, M.R</creatorcontrib><creatorcontrib>Chrispeels, M.J</creatorcontrib><title>Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion</title><title>Plant physiology (Bethesda)</title><description>Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.</description><subject>Azides</subject><subject>botany</subject><subject>Cell walls</subject><subject>Cyanides</subject><subject>Cytoplasm</subject><subject>Exocrine cells</subject><subject>Plant Science and Plant Products</subject><subject>Plants</subject><subject>Protein secretion</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Secretion</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><recordid>eNpFUG1LwzAYDKLgnP4DwfyBjrw0bfJRhi-FgR82P5c0fbJltMlMMrD_3soE4eAOjrvn4a7QggrOCiZKeY0WhMyaSKlu0V1KR0II5bRcoGk7-XyA5BLWvscJTITsgsfB4sPUx_A9nWIYnIfCBJ-1887v8ahNDGMYwJwHSNh5bHSMIacVbppmhUfIuptTBkf4OrsII_icsA3x_8I9urF6SPDwx0u0e33Zrd-Lzcdbs37eFLYUqhAdk8CMoSUR8_vM8Mpo6IBUNSjSc80IA2sqpWoupeE9UdLYrq-10orPWKLHS-0x5RDbU3SjjlNbsorR-td-uthWh1bvo0vt55bN4xBKK8UE4z-oUGMX</recordid><startdate>19700901</startdate><enddate>19700901</enddate><creator>Doerschug, M.R</creator><creator>Chrispeels, M.J</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope></search><sort><creationdate>19700901</creationdate><title>Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion</title><author>Doerschug, M.R ; Chrispeels, M.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f459-5b28e2cc14050882c36caebe067e90d3a202efc6997388c3d098cfbd7a9a93a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Azides</topic><topic>botany</topic><topic>Cell walls</topic><topic>Cyanides</topic><topic>Cytoplasm</topic><topic>Exocrine cells</topic><topic>Plant Science and Plant Products</topic><topic>Plants</topic><topic>Protein secretion</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doerschug, M.R</creatorcontrib><creatorcontrib>Chrispeels, M.J</creatorcontrib><collection>AGRIS</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doerschug, M.R</au><au>Chrispeels, M.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>1970-09-01</date><risdate>1970</risdate><volume>46</volume><issue>3</issue><spage>363</spage><epage>366</epage><pages>363-366</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.</abstract><pub>American Society of Plant Physiologists</pub><tpages>4</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0032-0889
ispartof	Plant physiology (Bethesda), 1970-09, Vol.46 (3), p.363-366
issn	0032-0889 1532-2548
language	eng
recordid	cdi_jstor_primary_4262179
source	Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection
subjects	Azides botany Cell walls Cyanides Cytoplasm Exocrine cells Plant Science and Plant Products Plants Protein secretion Protein synthesis Proteins Secretion
title	Synthesis and secretion of hydroxyproline-containing macromolecules in carrots. III. metabolic requirements for secretion
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T20%3A40%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_fao_a&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synthesis%20and%20secretion%20of%20hydroxyproline-containing%20macromolecules%20in%20carrots.%20III.%20metabolic%20requirements%20for%20secretion&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Doerschug,%20M.R&rft.date=1970-09-01&rft.volume=46&rft.issue=3&rft.spage=363&rft.epage=366&rft.pages=363-366&rft.issn=0032-0889&rft.eissn=1532-2548&rft_id=info:doi/&rft_dat=%3Cjstor_fao_a%3E4262179%3C/jstor_fao_a%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_jstor_id=4262179&rfr_iscdi=true