RecA-like proteins are components of early meiotic nodules in lily
Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA hom...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-06, Vol.94 (13), p.6868-6873 |
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| creator | Anderson, L.K. (Wageningen Agricultural University, Netherlands.) Offenberg, H.H Verkuijlen, W.M.H.C Heyting, C |
| description | Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA homology before synaptic initiation. Two potential components of early nodules are Rad51 and Dmc1 proteins. These proteins are important for meiotic recombination in eukaryotes and are homologous to RecA, the major protein that catalyzes homologous pairing and DNA strand exchange in prokaryotes. In addition, Rad51 has been localized by immunofluorescence in abundant foci that may correspond to early nodules in yeast, lily, and mouse. In yeast and lily, Dmc1 and Lim15, the lily homolog of Dmc1, colocalize with Rad51. Here, using electron microscopic immunogold localization to spreads of zygotene and early pachytene SCs from lily, we confirm that RecA-like proteins are components of early nodules. The antibody used was generated to full-length tomato Rad51 protein and binds to both Rad51 and Lim15 in immunoblots of lily primary microsporocyte proteins. The labeled early nodules are heterogeneous in size and are associated with both axial elements and SCs. There are two classes of early nodules, those that are densely labeled with gold and those that are not labeled at all. This result may be due to technical limitations associated with using spread preparations or to differences in the nodules themselves. The presence of Rad51 and/or Lim15 proteins in early nodules supports the hypothesis that early nodules are involved in recombination-related events during meiosis. |
| doi_str_mv | 10.1073/pnas.94.13.6868 |
| format | Article |
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(Wageningen Agricultural University, Netherlands.) ; Offenberg, H.H ; Verkuijlen, W.M.H.C ; Heyting, C</creator><creatorcontrib>Anderson, L.K. (Wageningen Agricultural University, Netherlands.) ; Offenberg, H.H ; Verkuijlen, W.M.H.C ; Heyting, C</creatorcontrib><description>Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA homology before synaptic initiation. Two potential components of early nodules are Rad51 and Dmc1 proteins. These proteins are important for meiotic recombination in eukaryotes and are homologous to RecA, the major protein that catalyzes homologous pairing and DNA strand exchange in prokaryotes. In addition, Rad51 has been localized by immunofluorescence in abundant foci that may correspond to early nodules in yeast, lily, and mouse. In yeast and lily, Dmc1 and Lim15, the lily homolog of Dmc1, colocalize with Rad51. Here, using electron microscopic immunogold localization to spreads of zygotene and early pachytene SCs from lily, we confirm that RecA-like proteins are components of early nodules. The antibody used was generated to full-length tomato Rad51 protein and binds to both Rad51 and Lim15 in immunoblots of lily primary microsporocyte proteins. The labeled early nodules are heterogeneous in size and are associated with both axial elements and SCs. There are two classes of early nodules, those that are densely labeled with gold and those that are not labeled at all. This result may be due to technical limitations associated with using spread preparations or to differences in the nodules themselves. The presence of Rad51 and/or Lim15 proteins in early nodules supports the hypothesis that early nodules are involved in recombination-related events during meiosis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.13.6868</identifier><identifier>PMID: 11038554</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>ADN ; ANTERA ; ANTHERE ; ANTHERS ; Antibodies ; APAREAMIENTO CROMOSOMICO ; APPARIEMENT CHROMOSOMIQUE ; BINDING PROTEINS ; Biological Sciences ; CHROMOSOME PAIRING ; Chromosomes ; DNA ; EPS ; Genetics ; IMMUNOCYTOCHEMISTRY ; IMMUNOLOGIE ; IMMUNOLOGY ; INMUNOLOGIA ; Laboratorium voor Erfelijkheidsleer ; Laboratory of Genetics ; LILIUM LONGIFLORUM ; LYCOPERSICON ESCULENTUM ; MEIOSE ; MEIOSIS ; MICROSPOROCYTES ; Nodules ; Pachytene stage ; POLEN ; POLLEN ; Prophase ; PROTEINAS AGLUTINANTES ; PROTEINE DE LIAISON ; Proteins ; RECOMBINACION ; RECOMBINAISON ; RECOMBINATION ; RECOMBINATION NODULES ; SYNAPTONEMAL COMPLEX ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-06, Vol.94 (13), p.6868-6873</ispartof><rights>Copyright 1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jun 24, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c657t-a42fdb151b8613c179880c5cdd0467e17b291c72cc4bb1719a79f95e5539c8293</citedby><cites>FETCH-LOGICAL-c657t-a42fdb151b8613c179880c5cdd0467e17b291c72cc4bb1719a79f95e5539c8293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42245$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42245$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11038554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, L.K. (Wageningen Agricultural University, Netherlands.)</creatorcontrib><creatorcontrib>Offenberg, H.H</creatorcontrib><creatorcontrib>Verkuijlen, W.M.H.C</creatorcontrib><creatorcontrib>Heyting, C</creatorcontrib><title>RecA-like proteins are components of early meiotic nodules in lily</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA homology before synaptic initiation. Two potential components of early nodules are Rad51 and Dmc1 proteins. These proteins are important for meiotic recombination in eukaryotes and are homologous to RecA, the major protein that catalyzes homologous pairing and DNA strand exchange in prokaryotes. In addition, Rad51 has been localized by immunofluorescence in abundant foci that may correspond to early nodules in yeast, lily, and mouse. In yeast and lily, Dmc1 and Lim15, the lily homolog of Dmc1, colocalize with Rad51. Here, using electron microscopic immunogold localization to spreads of zygotene and early pachytene SCs from lily, we confirm that RecA-like proteins are components of early nodules. The antibody used was generated to full-length tomato Rad51 protein and binds to both Rad51 and Lim15 in immunoblots of lily primary microsporocyte proteins. The labeled early nodules are heterogeneous in size and are associated with both axial elements and SCs. There are two classes of early nodules, those that are densely labeled with gold and those that are not labeled at all. This result may be due to technical limitations associated with using spread preparations or to differences in the nodules themselves. The presence of Rad51 and/or Lim15 proteins in early nodules supports the hypothesis that early nodules are involved in recombination-related events during meiosis.</description><subject>ADN</subject><subject>ANTERA</subject><subject>ANTHERE</subject><subject>ANTHERS</subject><subject>Antibodies</subject><subject>APAREAMIENTO CROMOSOMICO</subject><subject>APPARIEMENT CHROMOSOMIQUE</subject><subject>BINDING PROTEINS</subject><subject>Biological Sciences</subject><subject>CHROMOSOME PAIRING</subject><subject>Chromosomes</subject><subject>DNA</subject><subject>EPS</subject><subject>Genetics</subject><subject>IMMUNOCYTOCHEMISTRY</subject><subject>IMMUNOLOGIE</subject><subject>IMMUNOLOGY</subject><subject>INMUNOLOGIA</subject><subject>Laboratorium voor Erfelijkheidsleer</subject><subject>Laboratory of Genetics</subject><subject>LILIUM LONGIFLORUM</subject><subject>LYCOPERSICON ESCULENTUM</subject><subject>MEIOSE</subject><subject>MEIOSIS</subject><subject>MICROSPOROCYTES</subject><subject>Nodules</subject><subject>Pachytene stage</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Prophase</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE DE LIAISON</subject><subject>Proteins</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>RECOMBINATION</subject><subject>RECOMBINATION NODULES</subject><subject>SYNAPTONEMAL COMPLEX</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhiMEotvCGQkJFPUAp2w9_ohtiUup-JIqIQE9W47jLF68drATyv57EmUpLQe4eCTP84w8foviCaA1IE7O-qDzWtI1kHUtanGvWAGSUNVUovvFCiHMK0ExPSqOc94ihCQT6GFxBICIYIyuitefrDmvvPtmyz7FwbqQS51saeKuj8GGIZexK61Ofl_urIuDM2WI7ehtLl0ovfP7R8WDTvtsHx_qSXH19s2Xi_fV5cd3Hy7OLytTMz5UmuKubYBBI2ogBrgUAhlm2hbRmlvgDZZgODaGNg1wkJrLTjLLGJFGYElOCrnMvdYbG1yYDhV0Mi6rqJ3yrkk67dX1mFTwc-nHJisiUD27rxZ3utvZ1kyLJe1Vn9xudmb_bie4r2oTfygMmMGkvzjoKX4fbR7UzmVjvdfBxjErLmvGAIv_glAjQphgE3j6F7iNYwrT_ymMgNSCCD5BZwtkUsw52e7mwYDUnL-a81eSKiBqzn8ynt_e8w9_CPwWMJu_23cmvPwnoLrR-8H-HCby6UJu8xDTDUoxpvN-z5Zmp6PSmzSFdPUZpOSIIQ6Y_ALg29cT</recordid><startdate>19970624</startdate><enddate>19970624</enddate><creator>Anderson, L.K. (Wageningen Agricultural University, Netherlands.)</creator><creator>Offenberg, H.H</creator><creator>Verkuijlen, W.M.H.C</creator><creator>Heyting, C</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences of the USA</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>QVL</scope></search><sort><creationdate>19970624</creationdate><title>RecA-like proteins are components of early meiotic nodules in lily</title><author>Anderson, L.K. 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(Wageningen Agricultural University, Netherlands.)</creatorcontrib><creatorcontrib>Offenberg, H.H</creatorcontrib><creatorcontrib>Verkuijlen, W.M.H.C</creatorcontrib><creatorcontrib>Heyting, C</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>NARCIS:Publications</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, L.K. (Wageningen Agricultural University, Netherlands.)</au><au>Offenberg, H.H</au><au>Verkuijlen, W.M.H.C</au><au>Heyting, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RecA-like proteins are components of early meiotic nodules in lily</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1997-06-24</date><risdate>1997</risdate><volume>94</volume><issue>13</issue><spage>6868</spage><epage>6873</epage><pages>6868-6873</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA homology before synaptic initiation. Two potential components of early nodules are Rad51 and Dmc1 proteins. These proteins are important for meiotic recombination in eukaryotes and are homologous to RecA, the major protein that catalyzes homologous pairing and DNA strand exchange in prokaryotes. In addition, Rad51 has been localized by immunofluorescence in abundant foci that may correspond to early nodules in yeast, lily, and mouse. In yeast and lily, Dmc1 and Lim15, the lily homolog of Dmc1, colocalize with Rad51. Here, using electron microscopic immunogold localization to spreads of zygotene and early pachytene SCs from lily, we confirm that RecA-like proteins are components of early nodules. The antibody used was generated to full-length tomato Rad51 protein and binds to both Rad51 and Lim15 in immunoblots of lily primary microsporocyte proteins. The labeled early nodules are heterogeneous in size and are associated with both axial elements and SCs. There are two classes of early nodules, those that are densely labeled with gold and those that are not labeled at all. This result may be due to technical limitations associated with using spread preparations or to differences in the nodules themselves. The presence of Rad51 and/or Lim15 proteins in early nodules supports the hypothesis that early nodules are involved in recombination-related events during meiosis.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>11038554</pmid><doi>10.1073/pnas.94.13.6868</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1997-06, Vol.94 (13), p.6868-6873 |
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| subjects | ADN ANTERA ANTHERE ANTHERS Antibodies APAREAMIENTO CROMOSOMICO APPARIEMENT CHROMOSOMIQUE BINDING PROTEINS Biological Sciences CHROMOSOME PAIRING Chromosomes DNA EPS Genetics IMMUNOCYTOCHEMISTRY IMMUNOLOGIE IMMUNOLOGY INMUNOLOGIA Laboratorium voor Erfelijkheidsleer Laboratory of Genetics LILIUM LONGIFLORUM LYCOPERSICON ESCULENTUM MEIOSE MEIOSIS MICROSPOROCYTES Nodules Pachytene stage POLEN POLLEN Prophase PROTEINAS AGLUTINANTES PROTEINE DE LIAISON Proteins RECOMBINACION RECOMBINAISON RECOMBINATION RECOMBINATION NODULES SYNAPTONEMAL COMPLEX Yeasts |
| title | RecA-like proteins are components of early meiotic nodules in lily |
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