EPR-kinetic isotope effect study of the mechanism of radical-mediated dehydrogenation of an alcohol by the radical SAM enzyme Desll

The radical S-adenosyl-L-methionine enzyme Desll from Streptomyces venezuelae is able to oxidize the C3 hydroxyl group of TDP-D-quinovose to the corresponding ketone via an a-hydroxyalkyl radical intermediate. It is unknown whether electron transfer from the radical intermediate precedes or follows...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2013-02, Vol.110 (6), p.2088-2093
Hauptverfasser:	Ruszczycky, Mark W., Choi, Sei-hyun, Liu, Hung-wen
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Dehydrogenation Deuterium Electron transfer Enzymes Free radicals Isotope effects Kinetics Oxidation Solvents
Online-Zugang:	Volltext
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