Combining Two Genomes in One Cell: Stable Cloning of the Synechocystis PCC6803 Genome in the Bacillus subtilis 168 Genome

Cloning the whole 3.5-megabase (Mb) genome of the photosynthetic bacterium Synechocystis PCC6803 into the 4.2-Mb genome of the mesophilic bacterium Bacillus subtilis 168 resulted in a 7.7-Mb composite genome. We succeeded in such unprecedented large-size cloning by progressively assembling and editi...

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