Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics

Igs offer a versatile template for combinatorial and rational design approaches to the de novo creation of catalytically active proteins. We have used a covalent capture selection strategy to identify biocatalysts from within a human semisynthetic antibody variable fragment library that uses a nucle...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2011-09, Vol.108 (38), p.15954-15959
Hauptverfasser:	Smirnov, Ivan, Carletti, Eugénie, Kurkova, Inna, Nachon, Florian, Nicolet, Yvain, Mitkevich, Vladimir A, Débat, Hélène, Avalle, Bérangère, Belogurov, Alexey A. Jr, Kuznetsov, Nikita, Reshetnyak, Andrey, Masson, Patrick, Tonevitsky, Alexander G, Ponomarenko, Natalia, Makarov, Alexander A, Friboulet, Alain, Tramontano, Alfonso, Gabibov, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Algorithms Amino Acid Sequence Animals Antibodies Antibodies - chemistry Antibodies - genetics Antibodies - metabolism Binding sites Binding Sites - genetics Biological Sciences Calorimetry Catalysis catalytic activity Chemical reactions CHO Cells Complementarity Determining Regions - chemistry Complementarity Determining Regions - genetics Complementarity Determining Regions - metabolism Cricetinae Cricetulus Crystallography, X-Ray dephosphorylation Enzyme kinetics Enzymes Esters Humans hydrogen bonding immunoglobulin G Immunoglobulin Light Chains - chemistry Immunoglobulin Light Chains - genetics Immunoglobulin Light Chains - metabolism Immunoglobulin Variable Region - chemistry Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - metabolism Kinetics Ligands light intensity Models, Molecular Molecular Sequence Data Molecules Mutation paraoxon Phosphonic acids Protein Binding Protein Conformation Proteins Proteins - chemistry Proteins - genetics Proteins - metabolism Reactivity Recombinant Proteins - chemistry Recombinant Proteins - metabolism Spectrometry, Mass, Electrospray Ionization Thermodynamics tyrosine
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