Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays
We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device witho...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2009-01, Vol.106 (1), p.79-84 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 106 |
| creator | Reisner, Walter Larsen, Niels B Flyvbjerg, Henrik Tegenfeldt, Jonas O Kristensen, Anders |
| description | We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into "connect-the-dots" conformations consisting of locally pinned segments joined by fluctuating linkers. These results have broad implications for nanotechnology fields that require methods for the nanoscale positioning and manipulation of DNA. |
| doi_str_mv | 10.1073/pnas.0811468106 |
| format | Article |
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By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into "connect-the-dots" conformations consisting of locally pinned segments joined by fluctuating linkers. 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| subjects | Biological Sciences Condensed Matter Physics Den kondenserade materiens fysik Dimers DNA DNA - chemistry DNA conformation Equipment Design Fluorescence Free energy Fysik Macromolecules Microfluidic Analytical Techniques - instrumentation Molecules nanofluidics Nanotechnology Nanotechnology - instrumentation Nanotechnology - methods Natural Sciences Naturvetenskap Nucleic Acid Conformation Physical Sciences Physics polymer confinement Polymers Self assembly |
| title | Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays |
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