Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120
Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-05, Vol.85 (9), p.3198-3202 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3202 |
|---|---|
| container_issue | 9 |
| container_start_page | 3198 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 85 |
| creator | Rusche, James R. Javaherian, Kashi McDanal, Charlene Petro, Joan Lynn, Debra L. Grimaila, Raymond Langlois, Alphonse Gallo, Robert C. Arthur, Larry O. Fischinger, Peter J. Bolognesi, Dani P. Putney, Scott D. Matthews, Thomas J. |
| description | Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120. |
| doi_str_mv | 10.1073/pnas.85.9.3198 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_cross</sourceid><recordid>TN_cdi_jstor_primary_31972</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>31972</jstor_id><sourcerecordid>31972</sourcerecordid><originalsourceid>FETCH-LOGICAL-c431t-d6d7ccf5a81c806802c5a909b8b87703c00998e3cf2308f4f3f42c643837f1183</originalsourceid><addsrcrecordid>eNp9kM1u1DAUhS0EKkNhywIJyQvUFQnXPxk7y2HU0pEqseBnazmOzbhK7CF2EH0DHhtHM4y6YuXF-e7xvR9CrwnUBAT7cAg61bKp25qRVj5BKwItqda8hadoBUBFJTnlz9GLlO4BoG0kXKALyhvKuVihP5uQfRd7bxPOe53xLux95zO-mZOPAUeHb-dRB7wbxznE3jpvvA3mAX_305yqXXDWZNvjrR2GhD_60GONKa_06EPEG-N7_MX-nMuIXcry3i6TesDX4Zcd4sG-xz8OhMJL9MzpIdlXp_cSfbu5_rq9re4-f9ptN3eV4Yzkql_3whjXaEmMhLUEahrdQtvJTgoBzJQTW2mZcZSBdNwxx6lZcyaZcIRIdomujr2HKZa1UlajT6Ysr4ONc1JCUuBFVAHrI2immNJknTpMftTTgyKgFvVqUa9ko1q1qC8Db0_Nczfa_oyfXJf83SnXyejBTToYn86YIMUCNI9qlvp_6eNvrv6XKzcPQ7a_cwHfHMH7lON0JgskKPsLG6urhw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78204000</pqid></control><display><type>article</type><title>Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Rusche, James R. ; Javaherian, Kashi ; McDanal, Charlene ; Petro, Joan ; Lynn, Debra L. ; Grimaila, Raymond ; Langlois, Alphonse ; Gallo, Robert C. ; Arthur, Larry O. ; Fischinger, Peter J. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</creator><creatorcontrib>Rusche, James R. ; Javaherian, Kashi ; McDanal, Charlene ; Petro, Joan ; Lynn, Debra L. ; Grimaila, Raymond ; Langlois, Alphonse ; Gallo, Robert C. ; Arthur, Larry O. ; Fischinger, Peter J. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</creatorcontrib><description>Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.85.9.3198</identifier><identifier>PMID: 2452447</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; Amino acids ; Animals ; Antibodies ; Antiserum ; Biological and medical sciences ; Blood ; Cell fusion ; Cellular immunity ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Giant cells ; HIV ; HIV - immunology ; HIV Envelope Protein gp120 ; Microbiology ; Pan troglodytes ; Proteins ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Retroviridae Proteins - immunology ; Viral Fusion Proteins - antagonists & inhibitors ; Virology ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-05, Vol.85 (9), p.3198-3202</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-d6d7ccf5a81c806802c5a909b8b87703c00998e3cf2308f4f3f42c643837f1183</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/31972$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/31972$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7112005$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2452447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rusche, James R.</creatorcontrib><creatorcontrib>Javaherian, Kashi</creatorcontrib><creatorcontrib>McDanal, Charlene</creatorcontrib><creatorcontrib>Petro, Joan</creatorcontrib><creatorcontrib>Lynn, Debra L.</creatorcontrib><creatorcontrib>Grimaila, Raymond</creatorcontrib><creatorcontrib>Langlois, Alphonse</creatorcontrib><creatorcontrib>Gallo, Robert C.</creatorcontrib><creatorcontrib>Arthur, Larry O.</creatorcontrib><creatorcontrib>Fischinger, Peter J.</creatorcontrib><creatorcontrib>Bolognesi, Dani P.</creatorcontrib><creatorcontrib>Putney, Scott D.</creatorcontrib><creatorcontrib>Matthews, Thomas J.</creatorcontrib><title>Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Cell fusion</subject><subject>Cellular immunity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Giant cells</subject><subject>HIV</subject><subject>HIV - immunology</subject><subject>HIV Envelope Protein gp120</subject><subject>Microbiology</subject><subject>Pan troglodytes</subject><subject>Proteins</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Retroviridae Proteins - immunology</subject><subject>Viral Fusion Proteins - antagonists & inhibitors</subject><subject>Virology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAUhS0EKkNhywIJyQvUFQnXPxk7y2HU0pEqseBnazmOzbhK7CF2EH0DHhtHM4y6YuXF-e7xvR9CrwnUBAT7cAg61bKp25qRVj5BKwItqda8hadoBUBFJTnlz9GLlO4BoG0kXKALyhvKuVihP5uQfRd7bxPOe53xLux95zO-mZOPAUeHb-dRB7wbxznE3jpvvA3mAX_305yqXXDWZNvjrR2GhD_60GONKa_06EPEG-N7_MX-nMuIXcry3i6TesDX4Zcd4sG-xz8OhMJL9MzpIdlXp_cSfbu5_rq9re4-f9ptN3eV4Yzkql_3whjXaEmMhLUEahrdQtvJTgoBzJQTW2mZcZSBdNwxx6lZcyaZcIRIdomujr2HKZa1UlajT6Ysr4ONc1JCUuBFVAHrI2immNJknTpMftTTgyKgFvVqUa9ko1q1qC8Db0_Nczfa_oyfXJf83SnXyejBTToYn86YIMUCNI9qlvp_6eNvrv6XKzcPQ7a_cwHfHMH7lON0JgskKPsLG6urhw</recordid><startdate>19880501</startdate><enddate>19880501</enddate><creator>Rusche, James R.</creator><creator>Javaherian, Kashi</creator><creator>McDanal, Charlene</creator><creator>Petro, Joan</creator><creator>Lynn, Debra L.</creator><creator>Grimaila, Raymond</creator><creator>Langlois, Alphonse</creator><creator>Gallo, Robert C.</creator><creator>Arthur, Larry O.</creator><creator>Fischinger, Peter J.</creator><creator>Bolognesi, Dani P.</creator><creator>Putney, Scott D.</creator><creator>Matthews, Thomas J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880501</creationdate><title>Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120</title><author>Rusche, James R. ; Javaherian, Kashi ; McDanal, Charlene ; Petro, Joan ; Lynn, Debra L. ; Grimaila, Raymond ; Langlois, Alphonse ; Gallo, Robert C. ; Arthur, Larry O. ; Fischinger, Peter J. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-d6d7ccf5a81c806802c5a909b8b87703c00998e3cf2308f4f3f42c643837f1183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Cell fusion</topic><topic>Cellular immunity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Giant cells</topic><topic>HIV</topic><topic>HIV - immunology</topic><topic>HIV Envelope Protein gp120</topic><topic>Microbiology</topic><topic>Pan troglodytes</topic><topic>Proteins</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Retroviridae Proteins - immunology</topic><topic>Viral Fusion Proteins - antagonists & inhibitors</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rusche, James R.</creatorcontrib><creatorcontrib>Javaherian, Kashi</creatorcontrib><creatorcontrib>McDanal, Charlene</creatorcontrib><creatorcontrib>Petro, Joan</creatorcontrib><creatorcontrib>Lynn, Debra L.</creatorcontrib><creatorcontrib>Grimaila, Raymond</creatorcontrib><creatorcontrib>Langlois, Alphonse</creatorcontrib><creatorcontrib>Gallo, Robert C.</creatorcontrib><creatorcontrib>Arthur, Larry O.</creatorcontrib><creatorcontrib>Fischinger, Peter J.</creatorcontrib><creatorcontrib>Bolognesi, Dani P.</creatorcontrib><creatorcontrib>Putney, Scott D.</creatorcontrib><creatorcontrib>Matthews, Thomas J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rusche, James R.</au><au>Javaherian, Kashi</au><au>McDanal, Charlene</au><au>Petro, Joan</au><au>Lynn, Debra L.</au><au>Grimaila, Raymond</au><au>Langlois, Alphonse</au><au>Gallo, Robert C.</au><au>Arthur, Larry O.</au><au>Fischinger, Peter J.</au><au>Bolognesi, Dani P.</au><au>Putney, Scott D.</au><au>Matthews, Thomas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-05-01</date><risdate>1988</risdate><volume>85</volume><issue>9</issue><spage>3198</spage><epage>3202</epage><pages>3198-3202</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2452447</pmid><doi>10.1073/pnas.85.9.3198</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-05, Vol.85 (9), p.3198-3202 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_31972 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | AIDS/HIV Amino Acid Sequence Amino acids Animals Antibodies Antiserum Biological and medical sciences Blood Cell fusion Cellular immunity Enzyme-Linked Immunosorbent Assay Epitopes Epitopes - analysis Fundamental and applied biological sciences. Psychology Giant cells HIV HIV - immunology HIV Envelope Protein gp120 Microbiology Pan troglodytes Proteins Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Retroviridae Proteins - immunology Viral Fusion Proteins - antagonists & inhibitors Virology Viruses |
| title | Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-amino Acid Sequence of the Viral Envelope, gp120 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T21%3A23%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antibodies%20that%20Inhibit%20Fusion%20of%20Human%20Immunodeficiency%20Virus-Infected%20Cells%20Bind%20a%2024-amino%20Acid%20Sequence%20of%20the%20Viral%20Envelope,%20gp120&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Rusche,%20James%20R.&rft.date=1988-05-01&rft.volume=85&rft.issue=9&rft.spage=3198&rft.epage=3202&rft.pages=3198-3202&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.85.9.3198&rft_dat=%3Cjstor_cross%3E31972%3C/jstor_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78204000&rft_id=info:pmid/2452447&rft_jstor_id=31972&rfr_iscdi=true |