Cloning and Functional Analysis of Two Gibberellin 3β-hydroxylase Genes That are Differently Expressed during the Growth of Rice

We have cloned two gibberellin (GA) 3β-hydroxylase genes, OsGA3ox1 and OsGA3ox2, from rice by screening a genomic library with a DNA fragment obtained by PCR using degenerate primers. We have used full-scan GC-MS and Kovats retention indices to show function for the two encoded recombinant fusion pr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2001-07, Vol.98 (15), p.8909-8914
Hauptverfasser:	Itoh, Hironori, Ueguchi-Tanaka, Miyako, Sentoku, Naoki, Kitano, Hidemi, Matsuoka, Makoto, Kobayashi, Masatomo
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Amino Acid Sequence Anthers Base Sequence Biological Sciences chromosome 1 chromosome 5 Cloning, Molecular D18 gene DNA DNA, Plant Gene Expression Gene Expression Profiling Genes Genetic loci Genomics gibberellin 3^b-hydroxylase Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Molecular Sequence Data Molecular Structure Oryza - enzymology Oryza - genetics Oryza - growth & development OsGA3ox1 gene OsGA3ox2 gene Pollen Polymerase chain reaction Rice RNA Sequence Homology, Amino Acid
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