Characterization of VP7 and VP4 Genes of Rotavirus Strains: 1990-1994 and 2000-2002
A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n = 77) and 2000-2002 (n = 13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by mon...
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| Veröffentlicht in: | Epidemiology and infection 2009-07, Vol.137 (7), p.936-942 |
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| creator | Zade, J. K. Chhabra, P. Chitambar, S. D. |
| description | A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n = 77) and 2000-2002 (n = 13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79·2% and 92·3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1·3% and 7·7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (G1P[6], G1P[4], G1P[19], G2P[8], G3P[4], G4P[6]) in 19·5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India. |
| doi_str_mv | 10.1017/S09502688001532 |
| format | Article |
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D.</creatorcontrib><description>A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n = 77) and 2000-2002 (n = 13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79·2% and 92·3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1·3% and 7·7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (G1P[6], G1P[4], G1P[19], G2P[8], G3P[4], G4P[6]) in 19·5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.</description><identifier>ISSN: 0950-2688</identifier><identifier>EISSN: 1469-4409</identifier><identifier>DOI: 10.1017/S09502688001532</identifier><language>eng</language><publisher>Cambridge University Press</publisher><subject>Children ; Diarrhea ; Gastroenteritis and Rotaviruses ; Genotypes ; Microbiology ; Nucleotides ; Polymerase chain reaction ; Rotavirus ; Sequencing ; Serotyping ; Virology</subject><ispartof>Epidemiology and infection, 2009-07, Vol.137 (7), p.936-942</ispartof><rights>Copyright 2009 Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30221699$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30221699$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids></links><search><creatorcontrib>Zade, J. K.</creatorcontrib><creatorcontrib>Chhabra, P.</creatorcontrib><creatorcontrib>Chitambar, S. D.</creatorcontrib><title>Characterization of VP7 and VP4 Genes of Rotavirus Strains: 1990-1994 and 2000-2002</title><title>Epidemiology and infection</title><description>A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n = 77) and 2000-2002 (n = 13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79·2% and 92·3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1·3% and 7·7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (G1P[6], G1P[4], G1P[19], G2P[8], G3P[4], G4P[6]) in 19·5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.</description><subject>Children</subject><subject>Diarrhea</subject><subject>Gastroenteritis and Rotaviruses</subject><subject>Genotypes</subject><subject>Microbiology</subject><subject>Nucleotides</subject><subject>Polymerase chain reaction</subject><subject>Rotavirus</subject><subject>Sequencing</subject><subject>Serotyping</subject><subject>Virology</subject><issn>0950-2688</issn><issn>1469-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjMsKwjAURIMoWB9rV0J-IHqTpo-4FR9LseK2BG0xRRNJoqBfbyru3cyBOcMgNKEwo0CzeQEiAZbmOQBNYtZBEeWpIJyD6KKolaS1fTRwrgEAwfIsQsXyIq08-cqqt_TKaGxqfNxlWOpzIMebSleuLffGy6eyD4cLb6XSboGpEEBC8O-ahVMSgo1Qr5ZXV41_HKLpenVYbknjvLHl3aqbtK8yBsZoKkT8z38ATss9CA</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Zade, J. K.</creator><creator>Chhabra, P.</creator><creator>Chitambar, S. D.</creator><general>Cambridge University Press</general><scope/></search><sort><creationdate>20090701</creationdate><title>Characterization of VP7 and VP4 Genes of Rotavirus Strains: 1990-1994 and 2000-2002</title><author>Zade, J. K. ; Chhabra, P. ; Chitambar, S. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-jstor_primary_302216993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Children</topic><topic>Diarrhea</topic><topic>Gastroenteritis and Rotaviruses</topic><topic>Genotypes</topic><topic>Microbiology</topic><topic>Nucleotides</topic><topic>Polymerase chain reaction</topic><topic>Rotavirus</topic><topic>Sequencing</topic><topic>Serotyping</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zade, J. K.</creatorcontrib><creatorcontrib>Chhabra, P.</creatorcontrib><creatorcontrib>Chitambar, S. D.</creatorcontrib><jtitle>Epidemiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zade, J. K.</au><au>Chhabra, P.</au><au>Chitambar, S. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of VP7 and VP4 Genes of Rotavirus Strains: 1990-1994 and 2000-2002</atitle><jtitle>Epidemiology and infection</jtitle><date>2009-07-01</date><risdate>2009</risdate><volume>137</volume><issue>7</issue><spage>936</spage><epage>942</epage><pages>936-942</pages><issn>0950-2688</issn><eissn>1469-4409</eissn><abstract>A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n = 77) and 2000-2002 (n = 13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79·2% and 92·3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1·3% and 7·7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (G1P[6], G1P[4], G1P[19], G2P[8], G3P[4], G4P[6]) in 19·5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.</abstract><pub>Cambridge University Press</pub><doi>10.1017/S09502688001532</doi></addata></record> |
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| ispartof | Epidemiology and infection, 2009-07, Vol.137 (7), p.936-942 |
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| source | PubMed Central; JSTOR |
| subjects | Children Diarrhea Gastroenteritis and Rotaviruses Genotypes Microbiology Nucleotides Polymerase chain reaction Rotavirus Sequencing Serotyping Virology |
| title | Characterization of VP7 and VP4 Genes of Rotavirus Strains: 1990-1994 and 2000-2002 |
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