Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases
Polymyxin B is an antibiotic that kills sensitive cells by disrupting their membranes. We have cloned a wild-type yeast gene that, when present on a high-copy-number plasmid, renders the cells resistant to the drug. The nucleotide sequence of this gene is presented. A single open reading frame withi...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-08, Vol.84 (16), p.5848-5852 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Boguslawski, G Polazzi, J.O |
| description | Polymyxin B is an antibiotic that kills sensitive cells by disrupting their membranes. We have cloned a wild-type yeast gene that, when present on a high-copy-number plasmid, renders the cells resistant to the drug. The nucleotide sequence of this gene is presented. A single open reading frame within the sequence has the potential to encode a polypeptide (molecular mass of 77.5 kDa) that shows strong homologies to polypeptides of the protein kinase family. The gene, PBS2, located on chromosome X, is not allelic to the previously described PBS1 gene (where PBS signifies polymyxin B sensitivity). Although pbs1 mutations confer resistance to high levels of polymyxin B, double mutants, pbs1 pbs2, are not resistant to the drug, indicating that PBS2 is essential for pbs1 activity. Models based on the proposed protein kinase activity of the PBS2 gene product are presented to explain the interaction between PBS1 and PBS2 gene products involved in conferring polymyxin B resistance on yeast cells. |
| doi_str_mv | 10.1073/pnas.84.16.5848 |
| format | Article |
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We have cloned a wild-type yeast gene that, when present on a high-copy-number plasmid, renders the cells resistant to the drug. The nucleotide sequence of this gene is presented. A single open reading frame within the sequence has the potential to encode a polypeptide (molecular mass of 77.5 kDa) that shows strong homologies to polypeptides of the protein kinase family. The gene, PBS2, located on chromosome X, is not allelic to the previously described PBS1 gene (where PBS signifies polymyxin B sensitivity). Although pbs1 mutations confer resistance to high levels of polymyxin B, double mutants, pbs1 pbs2, are not resistant to the drug, indicating that PBS2 is essential for pbs1 activity. Models based on the proposed protein kinase activity of the PBS2 gene product are presented to explain the interaction between PBS1 and PBS2 gene products involved in conferring polymyxin B resistance on yeast cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.16.5848</identifier><identifier>PMID: 3039511</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; antibiotic resistance ; ANTIBIOTICOS ; ANTIBIOTICS ; ANTIBIOTIQUE ; Base Sequence ; Biological and medical sciences ; CELLS ; CELLULE ; CELULAS ; Cloning, Molecular ; Cots ; Diploidy ; DNA ; DNA, Fungal - analysis ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes. Genome ; GENETICA ; GENETICS ; GENETIQUE ; Gin ; Molecular and cellular biology ; Molecular genetics ; NUCLEOTIDE ; nucleotide sequence ; NUCLEOTIDES ; NUCLEOTIDOS ; Oats ; PEPTIDE ; PEPTIDES ; Peptides - genetics ; PEPTIDOS ; Plasmids ; polymyxin B ; Polymyxin B - pharmacology ; Polymyxins ; Polymyxins - pharmacology ; Protein Kinases - genetics ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-08, Vol.84 (16), p.5848-5852</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-998da1406778c2be5798e5fe3507f3de3823397f7b1526db18fb9994311a2573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/16.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30137$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30137$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27931,27932,53798,53800,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8342452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3039511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boguslawski, G</creatorcontrib><creatorcontrib>Polazzi, J.O</creatorcontrib><title>Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Polymyxin B is an antibiotic that kills sensitive cells by disrupting their membranes. We have cloned a wild-type yeast gene that, when present on a high-copy-number plasmid, renders the cells resistant to the drug. The nucleotide sequence of this gene is presented. A single open reading frame within the sequence has the potential to encode a polypeptide (molecular mass of 77.5 kDa) that shows strong homologies to polypeptides of the protein kinase family. The gene, PBS2, located on chromosome X, is not allelic to the previously described PBS1 gene (where PBS signifies polymyxin B sensitivity). Although pbs1 mutations confer resistance to high levels of polymyxin B, double mutants, pbs1 pbs2, are not resistant to the drug, indicating that PBS2 is essential for pbs1 activity. Models based on the proposed protein kinase activity of the PBS2 gene product are presented to explain the interaction between PBS1 and PBS2 gene products involved in conferring polymyxin B resistance on yeast cells.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>antibiotic resistance</subject><subject>ANTIBIOTICOS</subject><subject>ANTIBIOTICS</subject><subject>ANTIBIOTIQUE</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CELLS</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>Cloning, Molecular</subject><subject>Cots</subject><subject>Diploidy</subject><subject>DNA</subject><subject>DNA, Fungal - analysis</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>Gin</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>NUCLEOTIDE</subject><subject>nucleotide sequence</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>Oats</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>Peptides - genetics</subject><subject>PEPTIDOS</subject><subject>Plasmids</subject><subject>polymyxin B</subject><subject>Polymyxin B - pharmacology</subject><subject>Polymyxins</subject><subject>Polymyxins - pharmacology</subject><subject>Protein Kinases - genetics</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktvEzEUhUcIVNLCGgkB8gLBKqkf47GNxKJEvKRKLChry5m5Tl0846ntoOZH8J_xJFFUNrDy4nznvo6r6hnBC4IFOx8HkxayXpBmwWUtH1QzghWZN7XCD6sZxlTMZU3rx9VpSjcYY8UlPqlOGGaKEzKrfi9DP3rIgIZN6yFk1wFKcLuBoQUULDJoDQOgNgwWYnTDGo3Bb_vtnRvQBxQhuZTNjh3QFkzK71ByvfMmurydCuRrQGOEzrUZup15hHHXJocihAyl0k9X9oD0pHpkjU_w9PCeVVefPl4tv8wvv33-ury4nLe8pnmulOwMqXEjhGzpCrhQErgFxrGwrAMmKWNKWLEinDbdiki7UkrVjBBDuWBn1ft92XGz6qFrYcjReD1G15u41cE4_bcyuGu9Dr80VVI1uPjfHPwxlEulrHuXWvDeDBA2SQvR0HJ1-l-Q1GUDrFgBz_dgG0NKEexxGIL1FLSegtay1qTRU9DF8fL-Dkf-kGzRXx90k1rjbSwhuXTEJCu_gk8Tvj1gU_2jeuyj7cb7DHe5kK_-SRbg-R64STnEewMRNp38xV60JmizjmWUH9-l4IKqRkr2B9qB3k4</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>Boguslawski, G</creator><creator>Polazzi, J.O</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870801</creationdate><title>Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases</title><author>Boguslawski, G ; Polazzi, J.O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-998da1406778c2be5798e5fe3507f3de3823397f7b1526db18fb9994311a2573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>antibiotic resistance</topic><topic>ANTIBIOTICOS</topic><topic>ANTIBIOTICS</topic><topic>ANTIBIOTIQUE</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CELLS</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>Cloning, Molecular</topic><topic>Cots</topic><topic>Diploidy</topic><topic>DNA</topic><topic>DNA, Fungal - analysis</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>Gin</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>NUCLEOTIDE</topic><topic>nucleotide sequence</topic><topic>NUCLEOTIDES</topic><topic>NUCLEOTIDOS</topic><topic>Oats</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>Peptides - genetics</topic><topic>PEPTIDOS</topic><topic>Plasmids</topic><topic>polymyxin B</topic><topic>Polymyxin B - pharmacology</topic><topic>Polymyxins</topic><topic>Polymyxins - pharmacology</topic><topic>Protein Kinases - genetics</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boguslawski, G</creatorcontrib><creatorcontrib>Polazzi, J.O</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boguslawski, G</au><au>Polazzi, J.O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>84</volume><issue>16</issue><spage>5848</spage><epage>5852</epage><pages>5848-5852</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Polymyxin B is an antibiotic that kills sensitive cells by disrupting their membranes. We have cloned a wild-type yeast gene that, when present on a high-copy-number plasmid, renders the cells resistant to the drug. The nucleotide sequence of this gene is presented. A single open reading frame within the sequence has the potential to encode a polypeptide (molecular mass of 77.5 kDa) that shows strong homologies to polypeptides of the protein kinase family. The gene, PBS2, located on chromosome X, is not allelic to the previously described PBS1 gene (where PBS signifies polymyxin B sensitivity). Although pbs1 mutations confer resistance to high levels of polymyxin B, double mutants, pbs1 pbs2, are not resistant to the drug, indicating that PBS2 is essential for pbs1 activity. Models based on the proposed protein kinase activity of the PBS2 gene product are presented to explain the interaction between PBS1 and PBS2 gene products involved in conferring polymyxin B resistance on yeast cells.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3039511</pmid><doi>10.1073/pnas.84.16.5848</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-08, Vol.84 (16), p.5848-5852 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_30137 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids antibiotic resistance ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Base Sequence Biological and medical sciences CELLS CELLULE CELULAS Cloning, Molecular Cots Diploidy DNA DNA, Fungal - analysis Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology Genes Genes. Genome GENETICA GENETICS GENETIQUE Gin Molecular and cellular biology Molecular genetics NUCLEOTIDE nucleotide sequence NUCLEOTIDES NUCLEOTIDOS Oats PEPTIDE PEPTIDES Peptides - genetics PEPTIDOS Plasmids polymyxin B Polymyxin B - pharmacology Polymyxins Polymyxins - pharmacology Protein Kinases - genetics SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Yeasts |
| title | Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases |
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