Gene tandem-mediated selection of coliphage lambda-receptive Agrobacterium, Pseudomonas, and Rhizobium strains
Agrobacterium, Pseudomonas, and Rhizobium spp. have been made receptive to coliphage λ . To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bac...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (10), p.3334-3338 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Ludwig, R.A |
| description | Agrobacterium, Pseudomonas, and Rhizobium spp. have been made receptive to coliphage λ . To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bacteria. Because the wild-type λ receptor was not expressed in these bacteria, a procedure called the lamB gene tandem protocol was used to select lamB alleles that expressed the λ receptor. This gene tandem protocol may have general use in adapting genes with nonselectable traits to different organisms. Agrobacterium, Pseudomonas, and Rhizobium strains carrying pTROY41613, which encoded a gene tandem-selected λ receptor, could be quantitatively transduced with λ -packaged cosmids. With the ability to confer λ receptivity on organisms, phage λ now becomes a general DNA delivery agent. |
| doi_str_mv | 10.1073/pnas.84.10.3334 |
| format | Article |
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To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bacteria. Because the wild-type λ receptor was not expressed in these bacteria, a procedure called the lamB gene tandem protocol was used to select lamB alleles that expressed the λ receptor. This gene tandem protocol may have general use in adapting genes with nonselectable traits to different organisms. Agrobacterium, Pseudomonas, and Rhizobium strains carrying pTROY41613, which encoded a gene tandem-selected λ receptor, could be quantitatively transduced with λ -packaged cosmids. 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Psychology ; Genes ; Genetic mutation ; GENETICA ; GENETICS ; GENETIQUE ; Microbiology ; phage lambda ; Phenotypes ; Plasmids ; PSEUDOMONAS ; Receptors ; RECOMBINACION ; RECOMBINAISON ; RECOMBINATION ; RHIZOBIUM ; transduction</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-05, Vol.84 (10), p.3334-3338</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-67fc252801e78db464bc7728c1e07025bbf93860d436b91f4cbe33d0304373ce3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/29388$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/29388$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8318779$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16593836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ludwig, R.A</creatorcontrib><title>Gene tandem-mediated selection of coliphage lambda-receptive Agrobacterium, Pseudomonas, and Rhizobium strains</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Agrobacterium, Pseudomonas, and Rhizobium spp. have been made receptive to coliphage λ . To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bacteria. Because the wild-type λ receptor was not expressed in these bacteria, a procedure called the lamB gene tandem protocol was used to select lamB alleles that expressed the λ receptor. This gene tandem protocol may have general use in adapting genes with nonselectable traits to different organisms. Agrobacterium, Pseudomonas, and Rhizobium strains carrying pTROY41613, which encoded a gene tandem-selected λ receptor, could be quantitatively transduced with λ -packaged cosmids. With the ability to confer λ receptivity on organisms, phage λ now becomes a general DNA delivery agent.</description><subject>ADN</subject><subject>AGROBACTERIUM</subject><subject>Alleles</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Bacteriophages</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences: Genetics</subject><subject>Cosmids</subject><subject>DNA</subject><subject>dna vectors</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>Microbiology</subject><subject>phage lambda</subject><subject>Phenotypes</subject><subject>Plasmids</subject><subject>PSEUDOMONAS</subject><subject>Receptors</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>RECOMBINATION</subject><subject>RHIZOBIUM</subject><subject>transduction</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNp9kUFv1DAQhSMEokvhjMQB5YDopdmOYyd2DhyqCgpSJRDQs-U4k11XiR1spwJ-PY52tV0uPVmj-ebNG78se01gTYDTi8mqsBYsFWtKKXuSrQg0pKhZA0-zFUDJC8FKdpK9COEOAJpKwPPshNRVQwWtV5m9Rot5VLbDsRixMypilwccUEfjbO76XLvBTFu1wXxQY9upwqPGKZp7zC833rVKR_RmHs_zbwHnzo0umTrPk2T-fWv-ujb18hC9Mja8zJ71agj4av-eZrefPv68-lzcfL3-cnV5U2jGWSxq3uuyKgUQ5KJrWc1azXkpNEHgUFZt2yf_NXSM1m1DeqZbpLQDCoxyqpGeZh92utPcpqs02rR_kJM3o_J_pFNG_t-xZis37l4mBVGzNH-2n_fu14whytEEjcOgLLo5SE5pBYQAJPL9oyRhnNaELZIXO1B7F4LH_uCGgFzClEuYUrClXsJME2-Pj3jg9-kl4N0eUEGroffKahMOnKBEcN4c6SwLDt3jRWePArKfhyHi75jINzvyLkTnD2iZ_IiHZq-cVBufrNz-EDz9FAj6D5Yo0rU</recordid><startdate>19870501</startdate><enddate>19870501</enddate><creator>Ludwig, R.A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870501</creationdate><title>Gene tandem-mediated selection of coliphage lambda-receptive Agrobacterium, Pseudomonas, and Rhizobium strains</title><author>Ludwig, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-67fc252801e78db464bc7728c1e07025bbf93860d436b91f4cbe33d0304373ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>ADN</topic><topic>AGROBACTERIUM</topic><topic>Alleles</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Bacteriophages</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences: Genetics</topic><topic>Cosmids</topic><topic>DNA</topic><topic>dna vectors</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic mutation</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>Microbiology</topic><topic>phage lambda</topic><topic>Phenotypes</topic><topic>Plasmids</topic><topic>PSEUDOMONAS</topic><topic>Receptors</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>RECOMBINATION</topic><topic>RHIZOBIUM</topic><topic>transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ludwig, R.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ludwig, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene tandem-mediated selection of coliphage lambda-receptive Agrobacterium, Pseudomonas, and Rhizobium strains</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-05-01</date><risdate>1987</risdate><volume>84</volume><issue>10</issue><spage>3334</spage><epage>3338</epage><pages>3334-3338</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Agrobacterium, Pseudomonas, and Rhizobium spp. have been made receptive to coliphage λ . To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bacteria. Because the wild-type λ receptor was not expressed in these bacteria, a procedure called the lamB gene tandem protocol was used to select lamB alleles that expressed the λ receptor. This gene tandem protocol may have general use in adapting genes with nonselectable traits to different organisms. Agrobacterium, Pseudomonas, and Rhizobium strains carrying pTROY41613, which encoded a gene tandem-selected λ receptor, could be quantitatively transduced with λ -packaged cosmids. With the ability to confer λ receptivity on organisms, phage λ now becomes a general DNA delivery agent.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16593836</pmid><doi>10.1073/pnas.84.10.3334</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-05, Vol.84 (10), p.3334-3338 |
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| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | ADN AGROBACTERIUM Alleles Bacteria Bacteriology Bacteriophages Biological and medical sciences Biological Sciences: Genetics Cosmids DNA dna vectors Escherichia coli Fundamental and applied biological sciences. Psychology Genes Genetic mutation GENETICA GENETICS GENETIQUE Microbiology phage lambda Phenotypes Plasmids PSEUDOMONAS Receptors RECOMBINACION RECOMBINAISON RECOMBINATION RHIZOBIUM transduction |
| title | Gene tandem-mediated selection of coliphage lambda-receptive Agrobacterium, Pseudomonas, and Rhizobium strains |
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