Determination of Microbial Activity of Estuarine Detritus by Relative Rates of Lipid Biosynthesis
Microorganisms colonizing allochthonous detritus (live oak leaves) incubated in a north Florida estuary show metabolic activity that can be assayed reproducibly by the incorporation of [32P]H3PO 4and [1-14C] sodium acetate into the lipids in a 2-h period without introducing subculture bias. Relative...
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| Veröffentlicht in: | Limnology and oceanography 1977-01, Vol.22 (6), p.1089-1099 |
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| container_end_page | 1099 |
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| container_issue | 6 |
| container_start_page | 1089 |
| container_title | Limnology and oceanography |
| container_volume | 22 |
| creator | White, David C. Bobbie, Ronald J. Morrison, Susan J. Oosterhof, Darlene K. Taylor, Cecile W. Meeter, Duane A. |
| description | Microorganisms colonizing allochthonous detritus (live oak leaves) incubated in a north Florida estuary show metabolic activity that can be assayed reproducibly by the incorporation of [32P]H3PO
4and [1-14C] sodium acetate into the lipids in a 2-h period without introducing subculture bias. Relatively uncolonized live oak leaves show only about 1% of the biosynthetic capacity of leaves incubated in the estuary for 1 week. Lipid synthesis is proportional to time for at least 2 h and also to detrital mass. Random sampling from pooled portions of many leaves greatly reduces the variance of activities from individual leaves. Rates of phospholipid synthesis paralleled the total extractable ATP and the alkaline phosphatase activity during a 6-week incubation of live oak leaves in Apalachicola Bay. Rates of14Cincorporation into lipids paraleled the respiratory and the α-D-mannosidase activities over the same period, suggesting that lipid biosynthesis is a reasonable measure of detrital microbial activity. |
| doi_str_mv | 10.4319/lo.1977.22.6.1089 |
| format | Article |
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4and [1-14C] sodium acetate into the lipids in a 2-h period without introducing subculture bias. Relatively uncolonized live oak leaves show only about 1% of the biosynthetic capacity of leaves incubated in the estuary for 1 week. Lipid synthesis is proportional to time for at least 2 h and also to detrital mass. Random sampling from pooled portions of many leaves greatly reduces the variance of activities from individual leaves. Rates of phospholipid synthesis paralleled the total extractable ATP and the alkaline phosphatase activity during a 6-week incubation of live oak leaves in Apalachicola Bay. Rates of14Cincorporation into lipids paraleled the respiratory and the α-D-mannosidase activities over the same period, suggesting that lipid biosynthesis is a reasonable measure of detrital microbial activity.</description><identifier>ISSN: 0024-3590</identifier><identifier>DOI: 10.4319/lo.1977.22.6.1089</identifier><language>eng</language><publisher>American Society of Limnology and Oceanography</publisher><subject>Acetates ; Biosynthesis ; Brackish water ; Estuaries ; Leaves ; Lipids ; Microbial activity ; Microorganisms ; Phospholipids ; Radiocarbon</subject><ispartof>Limnology and oceanography, 1977-01, Vol.22 (6), p.1089-1099</ispartof><rights>Copyright 1977 American Society of Limnology and Oceanography, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2835183$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2835183$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids></links><search><creatorcontrib>White, David C.</creatorcontrib><creatorcontrib>Bobbie, Ronald J.</creatorcontrib><creatorcontrib>Morrison, Susan J.</creatorcontrib><creatorcontrib>Oosterhof, Darlene K.</creatorcontrib><creatorcontrib>Taylor, Cecile W.</creatorcontrib><creatorcontrib>Meeter, Duane A.</creatorcontrib><title>Determination of Microbial Activity of Estuarine Detritus by Relative Rates of Lipid Biosynthesis</title><title>Limnology and oceanography</title><description>Microorganisms colonizing allochthonous detritus (live oak leaves) incubated in a north Florida estuary show metabolic activity that can be assayed reproducibly by the incorporation of [32P]H3PO
4and [1-14C] sodium acetate into the lipids in a 2-h period without introducing subculture bias. Relatively uncolonized live oak leaves show only about 1% of the biosynthetic capacity of leaves incubated in the estuary for 1 week. Lipid synthesis is proportional to time for at least 2 h and also to detrital mass. Random sampling from pooled portions of many leaves greatly reduces the variance of activities from individual leaves. Rates of phospholipid synthesis paralleled the total extractable ATP and the alkaline phosphatase activity during a 6-week incubation of live oak leaves in Apalachicola Bay. Rates of14Cincorporation into lipids paraleled the respiratory and the α-D-mannosidase activities over the same period, suggesting that lipid biosynthesis is a reasonable measure of detrital microbial activity.</description><subject>Acetates</subject><subject>Biosynthesis</subject><subject>Brackish water</subject><subject>Estuaries</subject><subject>Leaves</subject><subject>Lipids</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Phospholipids</subject><subject>Radiocarbon</subject><issn>0024-3590</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotj81Kw0AcxPegYK0-gOBhXyBxv5LNHmutVYgIRc9lP_Ef0qTsbgt5e1P0NDAzv4FB6IGSUnCqnvqxpErKkrGyLilp1BVaEMJEwStFbtBtSh0hRFVVtUD6xWcfDzDoDOOAx4A_wMbRgO7xymY4Q54u7iblk44weDwDEfIpYTPhne9n7uzxTmefLr0WjuDwM4xpGvKPT5Du0HXQffL3_7pE36-br_Vb0X5u39ertuiorHMRiDXBMxcaKwzVdj7irHXGEcpE7akOTDQscCmlUlqbORbCyZopE5hVli_R499ul_IY98cIBx2nPWt4RRvOfwGc_FTv</recordid><startdate>19770101</startdate><enddate>19770101</enddate><creator>White, David C.</creator><creator>Bobbie, Ronald J.</creator><creator>Morrison, Susan J.</creator><creator>Oosterhof, Darlene K.</creator><creator>Taylor, Cecile W.</creator><creator>Meeter, Duane A.</creator><general>American Society of Limnology and Oceanography</general><scope/></search><sort><creationdate>19770101</creationdate><title>Determination of Microbial Activity of Estuarine Detritus by Relative Rates of Lipid Biosynthesis</title><author>White, David C. ; Bobbie, Ronald J. ; Morrison, Susan J. ; Oosterhof, Darlene K. ; Taylor, Cecile W. ; Meeter, Duane A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j176t-f0cbfe2df8c4b1ac319dccdbd01246e1af2482f377799aab19d44d7629bf2c9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Acetates</topic><topic>Biosynthesis</topic><topic>Brackish water</topic><topic>Estuaries</topic><topic>Leaves</topic><topic>Lipids</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Phospholipids</topic><topic>Radiocarbon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>White, David C.</creatorcontrib><creatorcontrib>Bobbie, Ronald J.</creatorcontrib><creatorcontrib>Morrison, Susan J.</creatorcontrib><creatorcontrib>Oosterhof, Darlene K.</creatorcontrib><creatorcontrib>Taylor, Cecile W.</creatorcontrib><creatorcontrib>Meeter, Duane A.</creatorcontrib><jtitle>Limnology and oceanography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>White, David C.</au><au>Bobbie, Ronald J.</au><au>Morrison, Susan J.</au><au>Oosterhof, Darlene K.</au><au>Taylor, Cecile W.</au><au>Meeter, Duane A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Microbial Activity of Estuarine Detritus by Relative Rates of Lipid Biosynthesis</atitle><jtitle>Limnology and oceanography</jtitle><date>1977-01-01</date><risdate>1977</risdate><volume>22</volume><issue>6</issue><spage>1089</spage><epage>1099</epage><pages>1089-1099</pages><issn>0024-3590</issn><abstract>Microorganisms colonizing allochthonous detritus (live oak leaves) incubated in a north Florida estuary show metabolic activity that can be assayed reproducibly by the incorporation of [32P]H3PO
4and [1-14C] sodium acetate into the lipids in a 2-h period without introducing subculture bias. Relatively uncolonized live oak leaves show only about 1% of the biosynthetic capacity of leaves incubated in the estuary for 1 week. Lipid synthesis is proportional to time for at least 2 h and also to detrital mass. Random sampling from pooled portions of many leaves greatly reduces the variance of activities from individual leaves. Rates of phospholipid synthesis paralleled the total extractable ATP and the alkaline phosphatase activity during a 6-week incubation of live oak leaves in Apalachicola Bay. Rates of14Cincorporation into lipids paraleled the respiratory and the α-D-mannosidase activities over the same period, suggesting that lipid biosynthesis is a reasonable measure of detrital microbial activity.</abstract><pub>American Society of Limnology and Oceanography</pub><doi>10.4319/lo.1977.22.6.1089</doi><tpages>11</tpages></addata></record> |
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| ispartof | Limnology and oceanography, 1977-01, Vol.22 (6), p.1089-1099 |
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| language | eng |
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| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acetates Biosynthesis Brackish water Estuaries Leaves Lipids Microbial activity Microorganisms Phospholipids Radiocarbon |
| title | Determination of Microbial Activity of Estuarine Detritus by Relative Rates of Lipid Biosynthesis |
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