The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins
The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospor...
Gespeichert in:
| Veröffentlicht in: | Mycologia 2016-05, Vol.108 (3), p.581-589 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 589 |
|---|---|
| container_issue | 3 |
| container_start_page | 581 |
| container_title | Mycologia |
| container_volume | 108 |
| creator | Heine, Daniela Petereit, Linda Schumann, Marcel R. Patzelt, Diana Rachid, Leila Brandt, Ulrike Werner, Antonia Pöggeler, Stefanie Fleißner, André |
| description | The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospora crassa, as a vacuolar membrane protein. The protein is detected only in aging and starving cultures and under other conditions, which induce autophagy, such as vegetative incompatibility or the presence of the macrolide antibiotic rapamycin. Mutant analysis revealed that TSP3 is dispensable for growth and development of the fungus under laboratory conditions. Together these findings indicate that tsp3 shares characteristics with idi (induced during incompatibility) genes and might promote vacuolar functions related to autophagy. |
| doi_str_mv | 10.3852/15-225 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_jstor_primary_26589325</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>26589325</jstor_id><sourcerecordid>26589325</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-cf8b8e50f7920b028f7fc501320b031ee05587745c472137fcd27a47a1006f8b3</originalsourceid><addsrcrecordid>eNqFkU9vFDEMxSMEotsC34Aqp4rLQP5MJpkjaku7UgVILOfIm020qWYm2zjTqt-elG1R1Qsny3o_P1vPhHzg7LM0SnzhqhFCvSILrpRuhJLda7JgTOhGKd4ekEPE69pWlb0lB6LrmenafkHm1dbT4ksG3MEUJ7r69VPSFOh3P-eEu5SBuioi0IgU6C24OQ2Q6ejHdYbJ011OxddBmDYUt5A9UlcLuOJzxBId0rtYtnR5tnxi8R15E2BA__6xHpHf385Xp5fN1Y-L5enXq8ZJ2ZfGBbM2XrGge8HWTJigg1OMy4dOcu-ZUkbrVrlWCy6ruBEaWg2csa7OyiPyae9bF9_MHosdIzo_DPXwNKPlhhndsq6T_0e1MUpqzbuKnuxRVxPC7IPd5ThCvrec2YdvWK5s_UYFjx895_XoN_-wp_gr8HEPXGNJ-ZmuTC__GvC9HqeQ8gh3KQ8bW-B-SDnU9F1EK18s_QPLQp3k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1788537716</pqid></control><display><type>article</type><title>The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>Alma/SFX Local Collection</source><creator>Heine, Daniela ; Petereit, Linda ; Schumann, Marcel R. ; Patzelt, Diana ; Rachid, Leila ; Brandt, Ulrike ; Werner, Antonia ; Pöggeler, Stefanie ; Fleißner, André</creator><creatorcontrib>Heine, Daniela ; Petereit, Linda ; Schumann, Marcel R. ; Patzelt, Diana ; Rachid, Leila ; Brandt, Ulrike ; Werner, Antonia ; Pöggeler, Stefanie ; Fleißner, André</creatorcontrib><description>The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospora crassa, as a vacuolar membrane protein. The protein is detected only in aging and starving cultures and under other conditions, which induce autophagy, such as vegetative incompatibility or the presence of the macrolide antibiotic rapamycin. Mutant analysis revealed that TSP3 is dispensable for growth and development of the fungus under laboratory conditions. Together these findings indicate that tsp3 shares characteristics with idi (induced during incompatibility) genes and might promote vacuolar functions related to autophagy.</description><identifier>ISSN: 0027-5514</identifier><identifier>EISSN: 1557-2536</identifier><identifier>DOI: 10.3852/15-225</identifier><identifier>PMID: 26908649</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Ascomycetes ; autophagy ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; idi gene ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Neurospora crassa ; Neurospora crassa - genetics ; Neurospora crassa - growth & development ; Neurospora crassa - metabolism ; ORGANELLES ; tetraspanin ; Tetraspanins - genetics ; Tetraspanins - metabolism ; tsp3 ; vacuole ; Vacuoles - genetics ; Vacuoles - metabolism</subject><ispartof>Mycologia, 2016-05, Vol.108 (3), p.581-589</ispartof><rights>2016 by The Mycological Society of America 2016</rights><rights>2016 by The Mycological Society of America</rights><rights>2016 by The Mycological Society of America.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c339t-cf8b8e50f7920b028f7fc501320b031ee05587745c472137fcd27a47a1006f8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26589325$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26589325$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26908649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heine, Daniela</creatorcontrib><creatorcontrib>Petereit, Linda</creatorcontrib><creatorcontrib>Schumann, Marcel R.</creatorcontrib><creatorcontrib>Patzelt, Diana</creatorcontrib><creatorcontrib>Rachid, Leila</creatorcontrib><creatorcontrib>Brandt, Ulrike</creatorcontrib><creatorcontrib>Werner, Antonia</creatorcontrib><creatorcontrib>Pöggeler, Stefanie</creatorcontrib><creatorcontrib>Fleißner, André</creatorcontrib><title>The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins</title><title>Mycologia</title><addtitle>Mycologia</addtitle><description>The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospora crassa, as a vacuolar membrane protein. The protein is detected only in aging and starving cultures and under other conditions, which induce autophagy, such as vegetative incompatibility or the presence of the macrolide antibiotic rapamycin. Mutant analysis revealed that TSP3 is dispensable for growth and development of the fungus under laboratory conditions. Together these findings indicate that tsp3 shares characteristics with idi (induced during incompatibility) genes and might promote vacuolar functions related to autophagy.</description><subject>Ascomycetes</subject><subject>autophagy</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>idi gene</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - genetics</subject><subject>Neurospora crassa - growth & development</subject><subject>Neurospora crassa - metabolism</subject><subject>ORGANELLES</subject><subject>tetraspanin</subject><subject>Tetraspanins - genetics</subject><subject>Tetraspanins - metabolism</subject><subject>tsp3</subject><subject>vacuole</subject><subject>Vacuoles - genetics</subject><subject>Vacuoles - metabolism</subject><issn>0027-5514</issn><issn>1557-2536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vFDEMxSMEotsC34Aqp4rLQP5MJpkjaku7UgVILOfIm020qWYm2zjTqt-elG1R1Qsny3o_P1vPhHzg7LM0SnzhqhFCvSILrpRuhJLda7JgTOhGKd4ekEPE69pWlb0lB6LrmenafkHm1dbT4ksG3MEUJ7r69VPSFOh3P-eEu5SBuioi0IgU6C24OQ2Q6ejHdYbJ011OxddBmDYUt5A9UlcLuOJzxBId0rtYtnR5tnxi8R15E2BA__6xHpHf385Xp5fN1Y-L5enXq8ZJ2ZfGBbM2XrGge8HWTJigg1OMy4dOcu-ZUkbrVrlWCy6ruBEaWg2csa7OyiPyae9bF9_MHosdIzo_DPXwNKPlhhndsq6T_0e1MUpqzbuKnuxRVxPC7IPd5ThCvrec2YdvWK5s_UYFjx895_XoN_-wp_gr8HEPXGNJ-ZmuTC__GvC9HqeQ8gh3KQ8bW-B-SDnU9F1EK18s_QPLQp3k</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Heine, Daniela</creator><creator>Petereit, Linda</creator><creator>Schumann, Marcel R.</creator><creator>Patzelt, Diana</creator><creator>Rachid, Leila</creator><creator>Brandt, Ulrike</creator><creator>Werner, Antonia</creator><creator>Pöggeler, Stefanie</creator><creator>Fleißner, André</creator><general>Taylor & Francis</general><general>Mycological Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20160501</creationdate><title>The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins</title><author>Heine, Daniela ; Petereit, Linda ; Schumann, Marcel R. ; Patzelt, Diana ; Rachid, Leila ; Brandt, Ulrike ; Werner, Antonia ; Pöggeler, Stefanie ; Fleißner, André</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-cf8b8e50f7920b028f7fc501320b031ee05587745c472137fcd27a47a1006f8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Ascomycetes</topic><topic>autophagy</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>idi gene</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - genetics</topic><topic>Neurospora crassa - growth & development</topic><topic>Neurospora crassa - metabolism</topic><topic>ORGANELLES</topic><topic>tetraspanin</topic><topic>Tetraspanins - genetics</topic><topic>Tetraspanins - metabolism</topic><topic>tsp3</topic><topic>vacuole</topic><topic>Vacuoles - genetics</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heine, Daniela</creatorcontrib><creatorcontrib>Petereit, Linda</creatorcontrib><creatorcontrib>Schumann, Marcel R.</creatorcontrib><creatorcontrib>Patzelt, Diana</creatorcontrib><creatorcontrib>Rachid, Leila</creatorcontrib><creatorcontrib>Brandt, Ulrike</creatorcontrib><creatorcontrib>Werner, Antonia</creatorcontrib><creatorcontrib>Pöggeler, Stefanie</creatorcontrib><creatorcontrib>Fleißner, André</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Mycologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heine, Daniela</au><au>Petereit, Linda</au><au>Schumann, Marcel R.</au><au>Patzelt, Diana</au><au>Rachid, Leila</au><au>Brandt, Ulrike</au><au>Werner, Antonia</au><au>Pöggeler, Stefanie</au><au>Fleißner, André</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins</atitle><jtitle>Mycologia</jtitle><addtitle>Mycologia</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>108</volume><issue>3</issue><spage>581</spage><epage>589</epage><pages>581-589</pages><issn>0027-5514</issn><eissn>1557-2536</eissn><abstract>The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospora crassa, as a vacuolar membrane protein. The protein is detected only in aging and starving cultures and under other conditions, which induce autophagy, such as vegetative incompatibility or the presence of the macrolide antibiotic rapamycin. Mutant analysis revealed that TSP3 is dispensable for growth and development of the fungus under laboratory conditions. Together these findings indicate that tsp3 shares characteristics with idi (induced during incompatibility) genes and might promote vacuolar functions related to autophagy.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>26908649</pmid><doi>10.3852/15-225</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-5514 |
| ispartof | Mycologia, 2016-05, Vol.108 (3), p.581-589 |
| issn | 0027-5514 1557-2536 |
| language | eng |
| recordid | cdi_jstor_primary_26589325 |
| source | MEDLINE; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | Ascomycetes autophagy Fungal Proteins - genetics Fungal Proteins - metabolism idi gene Membrane Proteins - genetics Membrane Proteins - metabolism Neurospora crassa Neurospora crassa - genetics Neurospora crassa - growth & development Neurospora crassa - metabolism ORGANELLES tetraspanin Tetraspanins - genetics Tetraspanins - metabolism tsp3 vacuole Vacuoles - genetics Vacuoles - metabolism |
| title | The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T19%3A14%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20tetraspanin%20TSP3%20of%20Neurospora%20crassa%20is%20a%20vacuolar%20membrane%20protein%20and%20shares%20characteristics%20with%20IDI%20proteins&rft.jtitle=Mycologia&rft.au=Heine,%20Daniela&rft.date=2016-05-01&rft.volume=108&rft.issue=3&rft.spage=581&rft.epage=589&rft.pages=581-589&rft.issn=0027-5514&rft.eissn=1557-2536&rft_id=info:doi/10.3852/15-225&rft_dat=%3Cjstor_proqu%3E26589325%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1788537716&rft_id=info:pmid/26908649&rft_jstor_id=26589325&rfr_iscdi=true |