Synaptic circuits and their variations within different columns in the visual system ofDrosophila
We reconstructed the synaptic circuits of seven columns in the second neuropil or medulla behind the fly’s compound eye. These neurons embody some of themost stereotyped circuits in one of the mostminiaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to st...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2015-11, Vol.112 (44), p.13711-13716 |
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| creator | Takemura, Shin-ya Xu, C. Shan Lu, Zhiyuan Rivlin, Patricia K. Parag, Toufiq Olbris, Donald J. Plaza, Stephen Zhao, Ting Katz, William T. Umayam, Lowell Weaver, Charlotte Hess, Harald F. Horne, Jane Anne Nunez-Iglesias, Juan Aniceto, Roxanne Chang, Lei-Ann Lauchie, Shirley Nasca, Ashley Ogundeyi, Omotara Sigmund, Christopher Takemura, Satoko Tran, Julie Langille, Carlie Le Lacheur, Kelsey McLin, Sari Shinomiya, Aya Chklovskii, Dmitri B. Meinertzhagen, Ian A. Scheffer, Louis K. |
| description | We reconstructed the synaptic circuits of seven columns in the second neuropil or medulla behind the fly’s compound eye. These neurons embody some of themost stereotyped circuits in one of the mostminiaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to study variations between circuits in the medulla’s neighboring columns. This variation in the number of synapses and the types of their synaptic partners has previously been little addressed because methods that visualize multiple circuits have not resolved detailed connections, and existing connectomic studies, which can see such connections, have not so far examined multiple reconstructions of the same circuit. Here, we address the omission by comparing the circuits common to all seven columns to assess variation in their connection strengths and the resultant rates of several different and distinct types of connection error. Error rates reveal that, overall, |
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Shan ; Lu, Zhiyuan ; Rivlin, Patricia K. ; Parag, Toufiq ; Olbris, Donald J. ; Plaza, Stephen ; Zhao, Ting ; Katz, William T. ; Umayam, Lowell ; Weaver, Charlotte ; Hess, Harald F. ; Horne, Jane Anne ; Nunez-Iglesias, Juan ; Aniceto, Roxanne ; Chang, Lei-Ann ; Lauchie, Shirley ; Nasca, Ashley ; Ogundeyi, Omotara ; Sigmund, Christopher ; Takemura, Satoko ; Tran, Julie ; Langille, Carlie ; Le Lacheur, Kelsey ; McLin, Sari ; Shinomiya, Aya ; Chklovskii, Dmitri B. ; Meinertzhagen, Ian A. ; Scheffer, Louis K.</creator><creatorcontrib>Takemura, Shin-ya ; Xu, C. Shan ; Lu, Zhiyuan ; Rivlin, Patricia K. ; Parag, Toufiq ; Olbris, Donald J. ; Plaza, Stephen ; Zhao, Ting ; Katz, William T. ; Umayam, Lowell ; Weaver, Charlotte ; Hess, Harald F. ; Horne, Jane Anne ; Nunez-Iglesias, Juan ; Aniceto, Roxanne ; Chang, Lei-Ann ; Lauchie, Shirley ; Nasca, Ashley ; Ogundeyi, Omotara ; Sigmund, Christopher ; Takemura, Satoko ; Tran, Julie ; Langille, Carlie ; Le Lacheur, Kelsey ; McLin, Sari ; Shinomiya, Aya ; Chklovskii, Dmitri B. ; Meinertzhagen, Ian A. ; Scheffer, Louis K.</creatorcontrib><description>We reconstructed the synaptic circuits of seven columns in the second neuropil or medulla behind the fly’s compound eye. These neurons embody some of themost stereotyped circuits in one of the mostminiaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to study variations between circuits in the medulla’s neighboring columns. This variation in the number of synapses and the types of their synaptic partners has previously been little addressed because methods that visualize multiple circuits have not resolved detailed connections, and existing connectomic studies, which can see such connections, have not so far examined multiple reconstructions of the same circuit. Here, we address the omission by comparing the circuits common to all seven columns to assess variation in their connection strengths and the resultant rates of several different and distinct types of connection error. Error rates reveal that, overall, <1% of contacts are not part of a consensus circuit, and we classify those contacts that supplement (E+) or are missing from it (E−). Autapses, in which the same cell is both presynaptic and postsynaptic at the same synapse, are occasionally seen; two cells in particular, Dm9 and Mi1, form ≥20-fold more autapses than do other neurons. These results delimit the accuracy of developmental events that establish and normally maintain synaptic circuits with such precision, and thereby address the operation of such circuits. 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These neurons embody some of themost stereotyped circuits in one of the mostminiaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to study variations between circuits in the medulla’s neighboring columns. This variation in the number of synapses and the types of their synaptic partners has previously been little addressed because methods that visualize multiple circuits have not resolved detailed connections, and existing connectomic studies, which can see such connections, have not so far examined multiple reconstructions of the same circuit. Here, we address the omission by comparing the circuits common to all seven columns to assess variation in their connection strengths and the resultant rates of several different and distinct types of connection error. Error rates reveal that, overall, <1% of contacts are not part of a consensus circuit, and we classify those contacts that supplement (E+) or are missing from it (E−). Autapses, in which the same cell is both presynaptic and postsynaptic at the same synapse, are occasionally seen; two cells in particular, Dm9 and Mi1, form ≥20-fold more autapses than do other neurons. These results delimit the accuracy of developmental events that establish and normally maintain synaptic circuits with such precision, and thereby address the operation of such circuits. 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Autapses, in which the same cell is both presynaptic and postsynaptic at the same synapse, are occasionally seen; two cells in particular, Dm9 and Mi1, form ≥20-fold more autapses than do other neurons. These results delimit the accuracy of developmental events that establish and normally maintain synaptic circuits with such precision, and thereby address the operation of such circuits. They also establish a precedent for error rates that will be required in the new science of connectomics.</abstract><pub>National Academy of Sciences</pub></addata></record> |
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| title | Synaptic circuits and their variations within different columns in the visual system ofDrosophila |
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