Cloning and Expression of cDNA for Salmon Growth Hormone in Escherichia coli
cDNA clones encoding chum salmon (Oncorhynchus keta) growth hormone (sGH) have been isolated from a cDNA library prepared from chum salmon pituitary gland poly(A)+RNA. Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recomb...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-07, Vol.82 (13), p.4306-4310 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Sekine, Susumu Mizukami, Tamio Nishi, Tatsunari Kuwana, Yoshihisa Saito, Akiko Sato, Moriyuki Itoh, Seiga Kawauchi, Hiroshi |
| description | cDNA clones encoding chum salmon (Oncorhynchus keta) growth hormone (sGH) have been isolated from a cDNA library prepared from chum salmon pituitary gland poly(A)+RNA. Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 22 amino acids. The 5′and 3′untranslated regions of the message were 64 and 426 bases long, respectively. Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria. The partially purified sGH from E. coli stimulated the growth of rainbow trout and the activity was indistinguishable from that of natural sGH. |
| doi_str_mv | 10.1073/pnas.82.13.4306 |
| format | Article |
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Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 22 amino acids. The 5′and 3′untranslated regions of the message were 64 and 426 bases long, respectively. Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria. The partially purified sGH from E. coli stimulated the growth of rainbow trout and the activity was indistinguishable from that of natural sGH.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.13.4306</identifier><identifier>PMID: 16593578</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Biological and medical sciences ; Biological Sciences: Applied Biology ; Biotechnology ; Codons ; Complementary DNA ; Fish ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Growth hormones ; Hormones ; Messenger RNA ; Methods. Procedures. 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Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 22 amino acids. The 5′and 3′untranslated regions of the message were 64 and 426 bases long, respectively. Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria. The partially purified sGH from E. coli stimulated the growth of rainbow trout and the activity was indistinguishable from that of natural sGH.</description><subject>Amino acids</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences: Applied Biology</subject><subject>Biotechnology</subject><subject>Codons</subject><subject>Complementary DNA</subject><subject>Fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Growth hormones</subject><subject>Hormones</subject><subject>Messenger RNA</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Nucleotide sequences</subject><subject>Plasmids</subject><subject>Salmon</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNp9kc1vEzEQxS0EoqFwRuKAfEDqadPx19o-cKhCaJEiOABny-v1Nq4cO9gbKP89GyWE9sLJGs_vzRvNQ-g1gTkByS63yda5onPC5pxB-wTNCGjStFzDUzQDoLJRnPIz9KLWOwDQQsFzdEZaoZmQaoZWi5hTSLfYph4v77fF1xpywnnA7sPnKzzkgr_auJm-rkv-Na7xTS5T5XFIeFnd2pfg1sFil2N4iZ4NNlb_6vieo-8fl98WN83qy_WnxdWqcYKqseGScM9byZWHTnSi7QlhmnJNHIi-F4Ptvbad11x3FqinruW8b9tOtYz1jLBz9P4wd7vrNr53Po3FRrMtYWPLb5NtMI87KazNbf5pmAYOdNJfHPUl_9j5OppNqM7HaJPPu2okYwK4pHoiLw-kK7nW4oeTCQGzT8DsEzCKGsLMPoFJ8fbhbv_448kn4N0RsNXZOBSbXKgnTlNFOJcP5uwN_nYfGV38FzDDLsbR348T-eZA3tUxlxNKhdSU_QH4eK-N</recordid><startdate>19850701</startdate><enddate>19850701</enddate><creator>Sekine, Susumu</creator><creator>Mizukami, Tamio</creator><creator>Nishi, Tatsunari</creator><creator>Kuwana, Yoshihisa</creator><creator>Saito, Akiko</creator><creator>Sato, Moriyuki</creator><creator>Itoh, Seiga</creator><creator>Kawauchi, Hiroshi</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850701</creationdate><title>Cloning and Expression of cDNA for Salmon Growth Hormone in Escherichia coli</title><author>Sekine, Susumu ; Mizukami, Tamio ; Nishi, Tatsunari ; Kuwana, Yoshihisa ; Saito, Akiko ; Sato, Moriyuki ; Itoh, Seiga ; Kawauchi, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-4714e46748e0b5b56d11392491c05dd5fade9abe949ba02e2c644d66b8633d313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Amino acids</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences: Applied Biology</topic><topic>Biotechnology</topic><topic>Codons</topic><topic>Complementary DNA</topic><topic>Fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Growth hormones</topic><topic>Hormones</topic><topic>Messenger RNA</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Nucleotide sequences</topic><topic>Plasmids</topic><topic>Salmon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sekine, Susumu</creatorcontrib><creatorcontrib>Mizukami, Tamio</creatorcontrib><creatorcontrib>Nishi, Tatsunari</creatorcontrib><creatorcontrib>Kuwana, Yoshihisa</creatorcontrib><creatorcontrib>Saito, Akiko</creatorcontrib><creatorcontrib>Sato, Moriyuki</creatorcontrib><creatorcontrib>Itoh, Seiga</creatorcontrib><creatorcontrib>Kawauchi, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sekine, Susumu</au><au>Mizukami, Tamio</au><au>Nishi, Tatsunari</au><au>Kuwana, Yoshihisa</au><au>Saito, Akiko</au><au>Sato, Moriyuki</au><au>Itoh, Seiga</au><au>Kawauchi, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Expression of cDNA for Salmon Growth Hormone in Escherichia coli</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-07-01</date><risdate>1985</risdate><volume>82</volume><issue>13</issue><spage>4306</spage><epage>4310</epage><pages>4306-4310</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>cDNA clones encoding chum salmon (Oncorhynchus keta) growth hormone (sGH) have been isolated from a cDNA library prepared from chum salmon pituitary gland poly(A)+RNA. Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 22 amino acids. The 5′and 3′untranslated regions of the message were 64 and 426 bases long, respectively. Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria. The partially purified sGH from E. coli stimulated the growth of rainbow trout and the activity was indistinguishable from that of natural sGH.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16593578</pmid><doi>10.1073/pnas.82.13.4306</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-07, Vol.82 (13), p.4306-4310 |
| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Biological and medical sciences Biological Sciences: Applied Biology Biotechnology Codons Complementary DNA Fish Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Growth hormones Hormones Messenger RNA Methods. Procedures. Technologies Molecular cloning Nucleotide sequences Plasmids Salmon |
| title | Cloning and Expression of cDNA for Salmon Growth Hormone in Escherichia coli |
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