DNA Sequence and Characterization of Human Class II Major Histocompatibility Complex β Chains from the DR1 Haplotype
Two HLA class II β -chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DRβ chain (2918.4) and a DQβ chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocyte...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-05, Vol.82 (10), p.3405-3409 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Bell, John I. Estess, Pila St. John, Thomas Saiki, Randall Watling, David L. Erlich, Henry A. McDevitt, Hugh O. |
| description | Two HLA class II β -chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DRβ chain (2918.4) and a DQβ chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DRα chain. The translation products were immunoprecipitated with a β -chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for β and α chains. Sequence comparisons of 2918.4 with previously published DRβ -chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQβ chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQβ sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes. |
| doi_str_mv | 10.1073/pnas.82.10.3405 |
| format | Article |
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They represent a DRβ chain (2918.4) and a DQβ chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DRα chain. The translation products were immunoprecipitated with a β -chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for β and α chains. Sequence comparisons of 2918.4 with previously published DRβ -chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQβ chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQβ sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.10.3405</identifier><identifier>PMID: 3858829</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell lines ; Complementary DNA ; DNA - genetics ; DNA probes ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gels ; Genes ; Genetics of the immune response ; Gin ; Haplotypes ; Histocompatibility Antigens Class II - genetics ; HLA-DR Antigens ; Immunobiology ; Immunoprecipitation ; Isoelectric Point ; Major Histocompatibility Complex ; Messenger RNA ; Molecular Weight ; Nucleic acids ; Oocytes ; Oocytes - physiology ; Xenopus laevis - physiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-05, Vol.82 (10), p.3405-3409</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-6ce51cb516c07890b8d9a9484e4c1aa2350a2dcf808383ee2141a104d840c71a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25592$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25592$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9197780$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3858829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bell, John I.</creatorcontrib><creatorcontrib>Estess, Pila</creatorcontrib><creatorcontrib>St. John, Thomas</creatorcontrib><creatorcontrib>Saiki, Randall</creatorcontrib><creatorcontrib>Watling, David L.</creatorcontrib><creatorcontrib>Erlich, Henry A.</creatorcontrib><creatorcontrib>McDevitt, Hugh O.</creatorcontrib><title>DNA Sequence and Characterization of Human Class II Major Histocompatibility Complex β Chains from the DR1 Haplotype</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Two HLA class II β -chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DRβ chain (2918.4) and a DQβ chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DRα chain. The translation products were immunoprecipitated with a β -chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for β and α chains. Sequence comparisons of 2918.4 with previously published DRβ -chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQβ chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQβ sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell lines</subject><subject>Complementary DNA</subject><subject>DNA - genetics</subject><subject>DNA probes</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gels</subject><subject>Genes</subject><subject>Genetics of the immune response</subject><subject>Gin</subject><subject>Haplotypes</subject><subject>Histocompatibility Antigens Class II - genetics</subject><subject>HLA-DR Antigens</subject><subject>Immunobiology</subject><subject>Immunoprecipitation</subject><subject>Isoelectric Point</subject><subject>Major Histocompatibility Complex</subject><subject>Messenger RNA</subject><subject>Molecular Weight</subject><subject>Nucleic acids</subject><subject>Oocytes</subject><subject>Oocytes - physiology</subject><subject>Xenopus laevis - physiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2O0zAUhSMEGsrAGgkJ5AWCVTp2YifOgsUoA7TSABI_a-vWdagrx87YDpryWDwIz4SjhgIbVtbR-c69Vz5Z9pjgJcF1eTFYCEteJLEsKWZ3sgXBDckr2uC72QLjos45Lej97EEIe4xxwzg-y85KzjgvmkU2Xr2_RJ_UzaisVAjsFrU78CCj8vo7RO0sch1ajT1Y1BoIAa3X6B3snUcrHaKTrh8SttFGxwNqkzLqFv38MY3RNqDOux7FnUJXHwlawWBcPAzqYXavAxPUo_k9z768ef25XeXXH96u28vrXDJWxbySihG5YaSSuOYN3vBtAw3lVFFJAIqSYSi2suOYl7xUqiCUAMF0yymWNYHyPHt1nDuMm15tpbLRgxGD1z34g3Cgxb-O1Tvx1X0TZVPXnKb8iznvXfqiEEWvg1TGgFVuDKKuSFlVZAIvjqD0LgSvutMOgsVUlJiKEryY9FRUSjz9-7QTPzeT_OezD0GC6TxYqcMJa8h0IU7Yyxmb5v92_-wR3WhMVLcxkc_-SybgyRHYp179iSgYa4ryF4cBvlQ</recordid><startdate>19850501</startdate><enddate>19850501</enddate><creator>Bell, John I.</creator><creator>Estess, Pila</creator><creator>St. John, Thomas</creator><creator>Saiki, Randall</creator><creator>Watling, David L.</creator><creator>Erlich, Henry A.</creator><creator>McDevitt, Hugh O.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850501</creationdate><title>DNA Sequence and Characterization of Human Class II Major Histocompatibility Complex β Chains from the DR1 Haplotype</title><author>Bell, John I. ; Estess, Pila ; St. John, Thomas ; Saiki, Randall ; Watling, David L. ; Erlich, Henry A. ; McDevitt, Hugh O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-6ce51cb516c07890b8d9a9484e4c1aa2350a2dcf808383ee2141a104d840c71a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell lines</topic><topic>Complementary DNA</topic><topic>DNA - genetics</topic><topic>DNA probes</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gels</topic><topic>Genes</topic><topic>Genetics of the immune response</topic><topic>Gin</topic><topic>Haplotypes</topic><topic>Histocompatibility Antigens Class II - genetics</topic><topic>HLA-DR Antigens</topic><topic>Immunobiology</topic><topic>Immunoprecipitation</topic><topic>Isoelectric Point</topic><topic>Major Histocompatibility Complex</topic><topic>Messenger RNA</topic><topic>Molecular Weight</topic><topic>Nucleic acids</topic><topic>Oocytes</topic><topic>Oocytes - physiology</topic><topic>Xenopus laevis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bell, John I.</creatorcontrib><creatorcontrib>Estess, Pila</creatorcontrib><creatorcontrib>St. John, Thomas</creatorcontrib><creatorcontrib>Saiki, Randall</creatorcontrib><creatorcontrib>Watling, David L.</creatorcontrib><creatorcontrib>Erlich, Henry A.</creatorcontrib><creatorcontrib>McDevitt, Hugh O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bell, John I.</au><au>Estess, Pila</au><au>St. John, Thomas</au><au>Saiki, Randall</au><au>Watling, David L.</au><au>Erlich, Henry A.</au><au>McDevitt, Hugh O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Sequence and Characterization of Human Class II Major Histocompatibility Complex β Chains from the DR1 Haplotype</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-05-01</date><risdate>1985</risdate><volume>82</volume><issue>10</issue><spage>3405</spage><epage>3409</epage><pages>3405-3409</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Two HLA class II β -chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DRβ chain (2918.4) and a DQβ chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DRα chain. The translation products were immunoprecipitated with a β -chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for β and α chains. Sequence comparisons of 2918.4 with previously published DRβ -chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQβ chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQβ sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3858829</pmid><doi>10.1073/pnas.82.10.3405</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Biological and medical sciences Cell lines Complementary DNA DNA - genetics DNA probes Female Fundamental and applied biological sciences. Psychology Fundamental immunology Gels Genes Genetics of the immune response Gin Haplotypes Histocompatibility Antigens Class II - genetics HLA-DR Antigens Immunobiology Immunoprecipitation Isoelectric Point Major Histocompatibility Complex Messenger RNA Molecular Weight Nucleic acids Oocytes Oocytes - physiology Xenopus laevis - physiology |
| title | DNA Sequence and Characterization of Human Class II Major Histocompatibility Complex β Chains from the DR1 Haplotype |
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