Cultured Adherent Cells from Marrow can Serve as Long-Lasting Precursor Cells for Bone, Cartilage, and Lung in Irradiated Mice
Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the ma...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1995-05, Vol.92 (11), p.4857-4861 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Pereira, R. F. Halford, K. W. O'Hara, M. D. Leeper, D. B. Sokolov, B. P. Pollard, M. D. Bagasra, O. Prockop, D. J. |
| description | Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. They suggest that cells may be particularly attractive targets for gene therapy ex vivo. |
| doi_str_mv | 10.1073/pnas.92.11.4857 |
| format | Article |
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F. ; Halford, K. W. ; O'Hara, M. D. ; Leeper, D. B. ; Sokolov, B. P. ; Pollard, M. D. ; Bagasra, O. ; Prockop, D. J.</creator><creatorcontrib>Pereira, R. F. ; Halford, K. W. ; O'Hara, M. D. ; Leeper, D. B. ; Sokolov, B. P. ; Pollard, M. D. ; Bagasra, O. ; Prockop, D. J.</creatorcontrib><description>Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. 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F.</creatorcontrib><creatorcontrib>Halford, K. W.</creatorcontrib><creatorcontrib>O'Hara, M. D.</creatorcontrib><creatorcontrib>Leeper, D. B.</creatorcontrib><creatorcontrib>Sokolov, B. P.</creatorcontrib><creatorcontrib>Pollard, M. D.</creatorcontrib><creatorcontrib>Bagasra, O.</creatorcontrib><creatorcontrib>Prockop, D. J.</creatorcontrib><title>Cultured Adherent Cells from Marrow can Serve as Long-Lasting Precursor Cells for Bone, Cartilage, and Lung in Irradiated Mice</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. They suggest that cells may be particularly attractive targets for gene therapy ex vivo.</description><subject>Adipocytes</subject><subject>Animals</subject><subject>B lymphocytes</subject><subject>Base Sequence</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - radiation effects</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells</subject><subject>Bone Marrow Transplantation</subject><subject>Bones</subject><subject>Cartilage - cytology</subject><subject>Cartilage - radiation effects</subject><subject>Cell Adhesion</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Cesium Radioisotopes</subject><subject>Chondrocytes</subject><subject>Collagen - biosynthesis</subject><subject>Collagen - genetics</subject><subject>Cultured cells</subject><subject>DNA Primers</subject><subject>Endothelial cells</subject><subject>Gene Expression</subject><subject>Hematopoietic stem cells</subject><subject>Humans</subject><subject>Lung - cytology</subject><subject>Lung - radiation effects</subject><subject>Mesenchymal stem cells</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Transgenic</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Stem Cells - cytology</subject><subject>T lymphocytes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS0EKkvhzAWQT3AhW4_jtWOJS4n4qJQKJOBsOclkmyprb22nwIW_Ha92u8CF04z0fm8-9Ah5CmwJTJVnW2fjUvMlwFJUK3WPLIBpKKTQ7D5ZMMZVUQkuHpJHMV4zxvSqYifkRCkJAsoF-VXPU5oD9vS8v8KALtEapynSIfgNvbQh-O-0s45-wXCL1EbaeLcuGhvT6Nb0c8BuDtGHO1fu3nqHr2ltQxonu86tdT1t5kyPjl6EYPvRprzwcuzwMXkw2Cnik0M9Jd_ev_tafyyaTx8u6vOm6FZKpEK2Fqq2BQ2lGLATnLeylxIHiaDaoRKqUkOllGhLDaAUgB1KaDlWqu9Fa8tT8mY_dzu3G-y7_Gewk9mGcWPDT-PtaP5V3Hhl1v7WCKiYzPaXB3vwNzPGZDZj7PLH1qGfo1GK61JpnsGzPdgFH2PA4bgCmNkFZnaBGc0NgNkFlh3P_77syB8Syvqrg74z3ql_BphhnqaEP1ImX_yXzMCzPXAdkw9HgpdSyXz_bzdotNU</recordid><startdate>19950523</startdate><enddate>19950523</enddate><creator>Pereira, R. F.</creator><creator>Halford, K. W.</creator><creator>O'Hara, M. D.</creator><creator>Leeper, D. B.</creator><creator>Sokolov, B. P.</creator><creator>Pollard, M. D.</creator><creator>Bagasra, O.</creator><creator>Prockop, D. J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950523</creationdate><title>Cultured Adherent Cells from Marrow can Serve as Long-Lasting Precursor Cells for Bone, Cartilage, and Lung in Irradiated Mice</title><author>Pereira, R. F. ; Halford, K. W. ; O'Hara, M. D. ; Leeper, D. B. ; Sokolov, B. P. ; Pollard, M. D. ; Bagasra, O. ; Prockop, D. 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F.</creatorcontrib><creatorcontrib>Halford, K. W.</creatorcontrib><creatorcontrib>O'Hara, M. D.</creatorcontrib><creatorcontrib>Leeper, D. B.</creatorcontrib><creatorcontrib>Sokolov, B. P.</creatorcontrib><creatorcontrib>Pollard, M. D.</creatorcontrib><creatorcontrib>Bagasra, O.</creatorcontrib><creatorcontrib>Prockop, D. J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, R. F.</au><au>Halford, K. W.</au><au>O'Hara, M. D.</au><au>Leeper, D. B.</au><au>Sokolov, B. P.</au><au>Pollard, M. D.</au><au>Bagasra, O.</au><au>Prockop, D. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultured Adherent Cells from Marrow can Serve as Long-Lasting Precursor Cells for Bone, Cartilage, and Lung in Irradiated Mice</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-05-23</date><risdate>1995</risdate><volume>92</volume><issue>11</issue><spage>4857</spage><epage>4861</epage><pages>4857-4861</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. They suggest that cells may be particularly attractive targets for gene therapy ex vivo.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7761413</pmid><doi>10.1073/pnas.92.11.4857</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1995-05, Vol.92 (11), p.4857-4861 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adipocytes Animals B lymphocytes Base Sequence Bone and Bones - cytology Bone and Bones - radiation effects Bone marrow Bone Marrow Cells Bone Marrow Transplantation Bones Cartilage - cytology Cartilage - radiation effects Cell Adhesion Cell Differentiation Cells, Cultured Cesium Radioisotopes Chondrocytes Collagen - biosynthesis Collagen - genetics Cultured cells DNA Primers Endothelial cells Gene Expression Hematopoietic stem cells Humans Lung - cytology Lung - radiation effects Mesenchymal stem cells Mice Mice, Inbred Strains Mice, Transgenic Molecular Sequence Data Polymerase Chain Reaction - methods Stem Cells - cytology T lymphocytes |
| title | Cultured Adherent Cells from Marrow can Serve as Long-Lasting Precursor Cells for Bone, Cartilage, and Lung in Irradiated Mice |
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