Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain
Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-07, Vol.90 (14), p.6611-6615 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Hogan, C.J Wein, H Wordeman, L Scholey, J.M Sawin, K.E Cande, W.Z |
| description | Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody localizes to central spindles. Upon ATP reactivation of spindle elongation, antigens recognized by the antibody are associated exclusively with the central spindle midzone where antiparallel microtubules of each half-spindle overlap. The antibody recognizes several polypeptides by immunoblot using isolated spindle extracts. One of these polypeptides behaves like kinesin with respect to nucleotide-specific binding to and release from taxol-stabilized microtubules. Preincubation of the spindle model with the peptide antibody inhibits subsequent ATP reactivation of spindle elongation. Coincubation of the peptide antibody with peptide antigen rescues spindle function. These results support a role for kinesin-related protein(s) in spindle elongation (anaphase B) of mitosis and suggest that one or several polypeptides that we have identified in spindle extracts may fulfill this function. |
| doi_str_mv | 10.1073/pnas.90.14.6611 |
| format | Article |
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We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody localizes to central spindles. Upon ATP reactivation of spindle elongation, antigens recognized by the antibody are associated exclusively with the central spindle midzone where antiparallel microtubules of each half-spindle overlap. The antibody recognizes several polypeptides by immunoblot using isolated spindle extracts. One of these polypeptides behaves like kinesin with respect to nucleotide-specific binding to and release from taxol-stabilized microtubules. Preincubation of the spindle model with the peptide antibody inhibits subsequent ATP reactivation of spindle elongation. Coincubation of the peptide antibody with peptide antigen rescues spindle function. These results support a role for kinesin-related protein(s) in spindle elongation (anaphase B) of mitosis and suggest that one or several polypeptides that we have identified in spindle extracts may fulfill this function.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.14.6611</identifier><identifier>PMID: 8341676</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Adenosine Triphosphate - pharmacology ; ALGAE ; Amino Acid Sequence ; Anaphase ; Anaphase - physiology ; Antibodies ; Antibodies - pharmacology ; Antibody Specificity ; ANTICORPS ; ANTICUERPOS ; Antigens ; BACILLARIOPHYCEAE ; Binding, Competitive ; Biological and medical sciences ; Cell biology ; Cell cycle, cell proliferation ; Cell division ; Cell physiology ; Cells ; Cylindrotheca fusiformis ; Diatoms ; Fundamental and applied biological sciences. Psychology ; Gels ; Immunity (Disease) ; Kinesin - immunology ; Kinesin - physiology ; Marine ; Microtubules ; MITOSE ; MITOSIS ; Mitotic spindle apparatus ; Molecular and cellular biology ; Molecular Sequence Data ; Nervous system ; PEPTIDE ; Peptide Fragments - immunology ; PEPTIDOS ; PROTEINAS ; PROTEINE ; Spindle Apparatus - drug effects ; Spindle Apparatus - physiology ; Spindle Apparatus - ultrastructure</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-07, Vol.90 (14), p.6611-6615</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Jul 15, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-aedadbd623d9a05889c1df8fc385eca57bfe767e7961a11fed4e2deafe7e3bca3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/14.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2362556$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2362556$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4883646$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8341676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hogan, C.J</creatorcontrib><creatorcontrib>Wein, H</creatorcontrib><creatorcontrib>Wordeman, L</creatorcontrib><creatorcontrib>Scholey, J.M</creatorcontrib><creatorcontrib>Sawin, K.E</creatorcontrib><creatorcontrib>Cande, W.Z</creatorcontrib><title>Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody localizes to central spindles. Upon ATP reactivation of spindle elongation, antigens recognized by the antibody are associated exclusively with the central spindle midzone where antiparallel microtubules of each half-spindle overlap. The antibody recognizes several polypeptides by immunoblot using isolated spindle extracts. One of these polypeptides behaves like kinesin with respect to nucleotide-specific binding to and release from taxol-stabilized microtubules. Preincubation of the spindle model with the peptide antibody inhibits subsequent ATP reactivation of spindle elongation. Coincubation of the peptide antibody with peptide antigen rescues spindle function. These results support a role for kinesin-related protein(s) in spindle elongation (anaphase B) of mitosis and suggest that one or several polypeptides that we have identified in spindle extracts may fulfill this function.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>ALGAE</subject><subject>Amino Acid Sequence</subject><subject>Anaphase</subject><subject>Anaphase - physiology</subject><subject>Antibodies</subject><subject>Antibodies - pharmacology</subject><subject>Antibody Specificity</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Antigens</subject><subject>BACILLARIOPHYCEAE</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Cell biology</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell division</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cylindrotheca fusiformis</subject><subject>Diatoms</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Immunity (Disease)</subject><subject>Kinesin - immunology</subject><subject>Kinesin - physiology</subject><subject>Marine</subject><subject>Microtubules</subject><subject>MITOSE</subject><subject>MITOSIS</subject><subject>Mitotic spindle apparatus</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Nervous system</subject><subject>PEPTIDE</subject><subject>Peptide Fragments - immunology</subject><subject>PEPTIDOS</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Spindle Apparatus - drug effects</subject><subject>Spindle Apparatus - physiology</subject><subject>Spindle Apparatus - ultrastructure</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2P0zAQxSMEWpaFMxICZCEEp3bt2HEciQta8bHSShxgz9YknrQuiR1sd0X563FoKR8HOFnj93ue8byieMjoktGan08O4rLJhVhKydit4pTRhi2kaOjt4pTSsl4oUYq7xb0YN5TSplL0pDhRXDBZy9NivHRr29pkvSO-J-BgWkNEEifrzIAEB-9W8EO2jtzYFDxpdwTIhFOyBrMj2dabHUlrSCRg51fOfsNIPluHMXtGn3wgxo9g3f3iTg9DxAeH86y4fvvm08X7xdWHd5cXr68WXSVVWgAaMK2RJTcN0EqppmOmV33HVYUdVHXbYy1rrBvJgLEejcDSIORb5G0H_Kx4tX932rYjmg5dCjDoKdgRwk57sPpPxdm1XvkbLWSjymx_cbAH_2WLMenRxg6HARz6bdR1pWpeV_S_IJOS1nnpGXz2F7jx2-DyDnRJGZeCsbnt-R7qgo8xYH8cmFE9p63ntHWTC6HntLPjye__PPKHeLP-_KBD7GDoA7jOxiMmlMqtZ-zlAZvf_6n-6qP77TAk_Joy-fSfZAYe74FNzLEfiZLLsqrmTo_2cg9ewyrkYa4_NoIzziT_Dq8A4Fo</recordid><startdate>19930715</startdate><enddate>19930715</enddate><creator>Hogan, C.J</creator><creator>Wein, H</creator><creator>Wordeman, L</creator><creator>Scholey, J.M</creator><creator>Sawin, K.E</creator><creator>Cande, W.Z</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930715</creationdate><title>Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain</title><author>Hogan, C.J ; Wein, H ; Wordeman, L ; Scholey, J.M ; Sawin, K.E ; Cande, W.Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-aedadbd623d9a05889c1df8fc385eca57bfe767e7961a11fed4e2deafe7e3bca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>ALGAE</topic><topic>Amino Acid Sequence</topic><topic>Anaphase</topic><topic>Anaphase - physiology</topic><topic>Antibodies</topic><topic>Antibodies - pharmacology</topic><topic>Antibody Specificity</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>Antigens</topic><topic>BACILLARIOPHYCEAE</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Cell biology</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell division</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cylindrotheca fusiformis</topic><topic>Diatoms</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Immunity (Disease)</topic><topic>Kinesin - immunology</topic><topic>Kinesin - physiology</topic><topic>Marine</topic><topic>Microtubules</topic><topic>MITOSE</topic><topic>MITOSIS</topic><topic>Mitotic spindle apparatus</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Nervous system</topic><topic>PEPTIDE</topic><topic>Peptide Fragments - immunology</topic><topic>PEPTIDOS</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Spindle Apparatus - drug effects</topic><topic>Spindle Apparatus - physiology</topic><topic>Spindle Apparatus - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hogan, C.J</creatorcontrib><creatorcontrib>Wein, H</creatorcontrib><creatorcontrib>Wordeman, L</creatorcontrib><creatorcontrib>Scholey, J.M</creatorcontrib><creatorcontrib>Sawin, K.E</creatorcontrib><creatorcontrib>Cande, W.Z</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hogan, C.J</au><au>Wein, H</au><au>Wordeman, L</au><au>Scholey, J.M</au><au>Sawin, K.E</au><au>Cande, W.Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-07-15</date><risdate>1993</risdate><volume>90</volume><issue>14</issue><spage>6611</spage><epage>6615</epage><pages>6611-6615</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody localizes to central spindles. Upon ATP reactivation of spindle elongation, antigens recognized by the antibody are associated exclusively with the central spindle midzone where antiparallel microtubules of each half-spindle overlap. The antibody recognizes several polypeptides by immunoblot using isolated spindle extracts. One of these polypeptides behaves like kinesin with respect to nucleotide-specific binding to and release from taxol-stabilized microtubules. Preincubation of the spindle model with the peptide antibody inhibits subsequent ATP reactivation of spindle elongation. Coincubation of the peptide antibody with peptide antigen rescues spindle function. These results support a role for kinesin-related protein(s) in spindle elongation (anaphase B) of mitosis and suggest that one or several polypeptides that we have identified in spindle extracts may fulfill this function.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8341676</pmid><doi>10.1073/pnas.90.14.6611</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1993-07, Vol.90 (14), p.6611-6615 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PMC (PubMed Central); Full-Text Journals in Chemistry (Open access); Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - pharmacology ALGAE Amino Acid Sequence Anaphase Anaphase - physiology Antibodies Antibodies - pharmacology Antibody Specificity ANTICORPS ANTICUERPOS Antigens BACILLARIOPHYCEAE Binding, Competitive Biological and medical sciences Cell biology Cell cycle, cell proliferation Cell division Cell physiology Cells Cylindrotheca fusiformis Diatoms Fundamental and applied biological sciences. Psychology Gels Immunity (Disease) Kinesin - immunology Kinesin - physiology Marine Microtubules MITOSE MITOSIS Mitotic spindle apparatus Molecular and cellular biology Molecular Sequence Data Nervous system PEPTIDE Peptide Fragments - immunology PEPTIDOS PROTEINAS PROTEINE Spindle Apparatus - drug effects Spindle Apparatus - physiology Spindle Apparatus - ultrastructure |
| title | Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain |
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