Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol
A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumula...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-04, Vol.89 (7), p.2600-2604 |
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creator | Tarczynski, M.C. (University of Arizona, Tucson, AZ) Jensen, R.G Bohnert, H.J |
description | A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants |
doi_str_mv | 10.1073/pnas.89.7.2600 |
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(University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</creator><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</creatorcontrib><description>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.7.2600</identifier><identifier>PMID: 1557364</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Bacteria ; Biological and medical sciences ; Biotechnology ; Carbohydrate metabolism ; CODE GENETIQUE ; CODIGO GENETICO ; Crop harvesting ; Dehydrogenases ; ESCHERICHIA ; Escherichia coli - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Genetics ; GENIE GENETIQUE ; INGENIERIA GENETICA ; Leaves ; Magnetic Resonance Spectroscopy ; MANNITOL ; Mannitol - metabolism ; Metabolism ; METABOLISME DES GLUCIDES ; METABOLISMO DE CARBOHIDRATOS ; Methods. Procedures. Technologies ; NICOTIANA ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana tabacum ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; Plant roots ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; Plants, Toxic ; Sugar Alcohol Dehydrogenases - genetics ; Sugar alcohols ; Sugars ; Tobacco ; Transformation, Genetic ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-04, Vol.89 (7), p.2600-2604</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Apr 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-95b7334113c030879da0528fbed1a31ff922f781557059a14f3b03f6e4f055193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2358939$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2358939$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5198471$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1557364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creatorcontrib><creatorcontrib>Jensen, R.G</creatorcontrib><creatorcontrib>Bohnert, H.J</creatorcontrib><title>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</description><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbohydrate metabolism</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>Crop harvesting</subject><subject>Dehydrogenases</subject><subject>ESCHERICHIA</subject><subject>Escherichia coli - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>GENIE GENETIQUE</subject><subject>INGENIERIA GENETICA</subject><subject>Leaves</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>MANNITOL</subject><subject>Mannitol - metabolism</subject><subject>Metabolism</subject><subject>METABOLISME DES GLUCIDES</subject><subject>METABOLISMO DE CARBOHIDRATOS</subject><subject>Methods. Procedures. Technologies</subject><subject>NICOTIANA</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana tabacum</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>Plant roots</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>Plants, Toxic</subject><subject>Sugar Alcohol Dehydrogenases - genetics</subject><subject>Sugar alcohols</subject><subject>Sugars</subject><subject>Tobacco</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxRtR1nX16kEUgoi3GStfkwS8yLp-wIIH3XPIpJOxh3QyJmlZ_3vTzDiOIngKxftV5VW9rnuMYYlB0Fe7aMpSqqVYkhXAne4cg8KLFVNwtzsHIGIhGWH3uwelbAFAcQln3RnmXNAVO--2V7e77EoZUkTJI4PWxlaXBxPQWMNbtHHRoSGimk0srRgsqqkxNqHgTF9ahXY59ZOt8wgTe9TEaZyCqYeZo4lxqCk87O55E4p7dHgvupt3V18uPyyuP73_ePnmemG5YnWh-FpQyjCmFihIoXoDnEi_dj02FHuvCPFCzhsAVwYzT9dA_coxD5xjRS-61_u5u2k9ut662MwHvcvDaPIPncyg_1Ti8FVv0nfNpIC5_eWhPadvkytVj0OxLgQTXZqKJs0lJpz8F8QrTKUgrIHP_wK3acqx3UATwJQDlbxByz1kcyolO380jEHPSes5aS2VFnpOujU8O13zN76PtukvDrop1gTfArRDOWLtUpIJfLLFPP6XevxG-ymE6m7ryX__BJv-dK9vS035CBDKpaLzWZ_sZW-SNpvcrNx8VlgqrDj9CZH-2tU</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creator><creator>Jensen, R.G</creator><creator>Bohnert, H.J</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TB</scope><scope>5PM</scope></search><sort><creationdate>19920401</creationdate><title>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</title><author>Tarczynski, M.C. (University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-95b7334113c030879da0528fbed1a31ff922f781557059a14f3b03f6e4f055193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbohydrate metabolism</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>Crop harvesting</topic><topic>Dehydrogenases</topic><topic>ESCHERICHIA</topic><topic>Escherichia coli - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>GENIE GENETIQUE</topic><topic>INGENIERIA GENETICA</topic><topic>Leaves</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>MANNITOL</topic><topic>Mannitol - metabolism</topic><topic>Metabolism</topic><topic>METABOLISME DES GLUCIDES</topic><topic>METABOLISMO DE CARBOHIDRATOS</topic><topic>Methods. Procedures. Technologies</topic><topic>NICOTIANA</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana tabacum</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>Plant roots</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>Plants, Toxic</topic><topic>Sugar Alcohol Dehydrogenases - genetics</topic><topic>Sugar alcohols</topic><topic>Sugars</topic><topic>Tobacco</topic><topic>Transformation, Genetic</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creatorcontrib><creatorcontrib>Jensen, R.G</creatorcontrib><creatorcontrib>Bohnert, H.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</au><au>Jensen, R.G</au><au>Bohnert, H.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>89</volume><issue>7</issue><spage>2600</spage><epage>2604</epage><pages>2600-2604</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1557364</pmid><doi>10.1073/pnas.89.7.2600</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
subjects | Bacteria Biological and medical sciences Biotechnology Carbohydrate metabolism CODE GENETIQUE CODIGO GENETICO Crop harvesting Dehydrogenases ESCHERICHIA Escherichia coli - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genetic engineering Genetic technics Genetic Vectors Genetics GENIE GENETIQUE INGENIERIA GENETICA Leaves Magnetic Resonance Spectroscopy MANNITOL Mannitol - metabolism Metabolism METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Methods. Procedures. Technologies NICOTIANA Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum OXIDORREDUCTASAS OXYDOREDUCTASE Plant roots PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants Plants, Toxic Sugar Alcohol Dehydrogenases - genetics Sugar alcohols Sugars Tobacco Transformation, Genetic Transgenic animals and transgenic plants Transgenic plants |
title | Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol |
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