Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol

A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumula...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1992-04, Vol.89 (7), p.2600-2604
Hauptverfasser: Tarczynski, M.C. (University of Arizona, Tucson, AZ), Jensen, R.G, Bohnert, H.J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2604
container_issue 7
container_start_page 2600
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 89
creator Tarczynski, M.C. (University of Arizona, Tucson, AZ)
Jensen, R.G
Bohnert, H.J
description A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants
doi_str_mv 10.1073/pnas.89.7.2600
format Article
fullrecord <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_jstor_primary_2358939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2358939</jstor_id><sourcerecordid>2358939</sourcerecordid><originalsourceid>FETCH-LOGICAL-c594t-95b7334113c030879da0528fbed1a31ff922f781557059a14f3b03f6e4f055193</originalsourceid><addsrcrecordid>eNqFkc2LFDEQxRtR1nX16kEUgoi3GStfkwS8yLp-wIIH3XPIpJOxh3QyJmlZ_3vTzDiOIngKxftV5VW9rnuMYYlB0Fe7aMpSqqVYkhXAne4cg8KLFVNwtzsHIGIhGWH3uwelbAFAcQln3RnmXNAVO--2V7e77EoZUkTJI4PWxlaXBxPQWMNbtHHRoSGimk0srRgsqqkxNqHgTF9ahXY59ZOt8wgTe9TEaZyCqYeZo4lxqCk87O55E4p7dHgvupt3V18uPyyuP73_ePnmemG5YnWh-FpQyjCmFihIoXoDnEi_dj02FHuvCPFCzhsAVwYzT9dA_coxD5xjRS-61_u5u2k9ut662MwHvcvDaPIPncyg_1Ti8FVv0nfNpIC5_eWhPadvkytVj0OxLgQTXZqKJs0lJpz8F8QrTKUgrIHP_wK3acqx3UATwJQDlbxByz1kcyolO380jEHPSes5aS2VFnpOujU8O13zN76PtukvDrop1gTfArRDOWLtUpIJfLLFPP6XevxG-ymE6m7ryX__BJv-dK9vS035CBDKpaLzWZ_sZW-SNpvcrNx8VlgqrDj9CZH-2tU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201350385</pqid></control><display><type>article</type><title>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Tarczynski, M.C. (University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</creator><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</creatorcontrib><description>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.7.2600</identifier><identifier>PMID: 1557364</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Bacteria ; Biological and medical sciences ; Biotechnology ; Carbohydrate metabolism ; CODE GENETIQUE ; CODIGO GENETICO ; Crop harvesting ; Dehydrogenases ; ESCHERICHIA ; Escherichia coli - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Genetics ; GENIE GENETIQUE ; INGENIERIA GENETICA ; Leaves ; Magnetic Resonance Spectroscopy ; MANNITOL ; Mannitol - metabolism ; Metabolism ; METABOLISME DES GLUCIDES ; METABOLISMO DE CARBOHIDRATOS ; Methods. Procedures. Technologies ; NICOTIANA ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana tabacum ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; Plant roots ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; Plants, Toxic ; Sugar Alcohol Dehydrogenases - genetics ; Sugar alcohols ; Sugars ; Tobacco ; Transformation, Genetic ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-04, Vol.89 (7), p.2600-2604</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Apr 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-95b7334113c030879da0528fbed1a31ff922f781557059a14f3b03f6e4f055193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2358939$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2358939$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=5198471$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1557364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creatorcontrib><creatorcontrib>Jensen, R.G</creatorcontrib><creatorcontrib>Bohnert, H.J</creatorcontrib><title>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</description><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbohydrate metabolism</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>Crop harvesting</subject><subject>Dehydrogenases</subject><subject>ESCHERICHIA</subject><subject>Escherichia coli - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>GENIE GENETIQUE</subject><subject>INGENIERIA GENETICA</subject><subject>Leaves</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>MANNITOL</subject><subject>Mannitol - metabolism</subject><subject>Metabolism</subject><subject>METABOLISME DES GLUCIDES</subject><subject>METABOLISMO DE CARBOHIDRATOS</subject><subject>Methods. Procedures. Technologies</subject><subject>NICOTIANA</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana tabacum</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>Plant roots</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>Plants, Toxic</subject><subject>Sugar Alcohol Dehydrogenases - genetics</subject><subject>Sugar alcohols</subject><subject>Sugars</subject><subject>Tobacco</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxRtR1nX16kEUgoi3GStfkwS8yLp-wIIH3XPIpJOxh3QyJmlZ_3vTzDiOIngKxftV5VW9rnuMYYlB0Fe7aMpSqqVYkhXAne4cg8KLFVNwtzsHIGIhGWH3uwelbAFAcQln3RnmXNAVO--2V7e77EoZUkTJI4PWxlaXBxPQWMNbtHHRoSGimk0srRgsqqkxNqHgTF9ahXY59ZOt8wgTe9TEaZyCqYeZo4lxqCk87O55E4p7dHgvupt3V18uPyyuP73_ePnmemG5YnWh-FpQyjCmFihIoXoDnEi_dj02FHuvCPFCzhsAVwYzT9dA_coxD5xjRS-61_u5u2k9ut662MwHvcvDaPIPncyg_1Ti8FVv0nfNpIC5_eWhPadvkytVj0OxLgQTXZqKJs0lJpz8F8QrTKUgrIHP_wK3acqx3UATwJQDlbxByz1kcyolO380jEHPSes5aS2VFnpOujU8O13zN76PtukvDrop1gTfArRDOWLtUpIJfLLFPP6XevxG-ymE6m7ryX__BJv-dK9vS035CBDKpaLzWZ_sZW-SNpvcrNx8VlgqrDj9CZH-2tU</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creator><creator>Jensen, R.G</creator><creator>Bohnert, H.J</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TB</scope><scope>5PM</scope></search><sort><creationdate>19920401</creationdate><title>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</title><author>Tarczynski, M.C. (University of Arizona, Tucson, AZ) ; Jensen, R.G ; Bohnert, H.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-95b7334113c030879da0528fbed1a31ff922f781557059a14f3b03f6e4f055193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbohydrate metabolism</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>Crop harvesting</topic><topic>Dehydrogenases</topic><topic>ESCHERICHIA</topic><topic>Escherichia coli - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>GENIE GENETIQUE</topic><topic>INGENIERIA GENETICA</topic><topic>Leaves</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>MANNITOL</topic><topic>Mannitol - metabolism</topic><topic>Metabolism</topic><topic>METABOLISME DES GLUCIDES</topic><topic>METABOLISMO DE CARBOHIDRATOS</topic><topic>Methods. Procedures. Technologies</topic><topic>NICOTIANA</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana tabacum</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>Plant roots</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>Plants, Toxic</topic><topic>Sugar Alcohol Dehydrogenases - genetics</topic><topic>Sugar alcohols</topic><topic>Sugars</topic><topic>Tobacco</topic><topic>Transformation, Genetic</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</creatorcontrib><creatorcontrib>Jensen, R.G</creatorcontrib><creatorcontrib>Bohnert, H.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tarczynski, M.C. (University of Arizona, Tucson, AZ)</au><au>Jensen, R.G</au><au>Bohnert, H.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>89</volume><issue>7</issue><spage>2600</spage><epage>2604</epage><pages>2600-2604</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exceeded 6 micromoles/g (fresh weight) in the haves and in the roots of some transformants, whereas this sugar alcohol was not detected in these organs of wild-type tobacco plants or of untransformed tobacco plants that underwent the same regeneration scheme. These experiments demonstrate that branchpoints in plant carbohydrate metabolism can be generated by which novel gene products can utilize endogenous substrates to divert metabolic energy into novel compounds. Additionally, the system described here allows for physiological studies in which the responses of wild-type and transgenic tobacco to various environmental stimuli can be compared directly. Such studies will facilitate our understanding of the roles of sugar alcohols (e.g., in stress tolerance) in higher plants</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1557364</pmid><doi>10.1073/pnas.89.7.2600</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1992-04, Vol.89 (7), p.2600-2604
issn 0027-8424
1091-6490
language eng
recordid cdi_jstor_primary_2358939
source MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects Bacteria
Biological and medical sciences
Biotechnology
Carbohydrate metabolism
CODE GENETIQUE
CODIGO GENETICO
Crop harvesting
Dehydrogenases
ESCHERICHIA
Escherichia coli - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetic engineering
Genetic technics
Genetic Vectors
Genetics
GENIE GENETIQUE
INGENIERIA GENETICA
Leaves
Magnetic Resonance Spectroscopy
MANNITOL
Mannitol - metabolism
Metabolism
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
Methods. Procedures. Technologies
NICOTIANA
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana tabacum
OXIDORREDUCTASAS
OXYDOREDUCTASE
Plant roots
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
Plants, Toxic
Sugar Alcohol Dehydrogenases - genetics
Sugar alcohols
Sugars
Tobacco
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
title Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T10%3A15%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20a%20bacterial%20mtlD%20gene%20in%20transgenic%20tobacco%20leads%20to%20production%20and%20accumulation%20of%20mannitol&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Tarczynski,%20M.C.%20(University%20of%20Arizona,%20Tucson,%20AZ)&rft.date=1992-04-01&rft.volume=89&rft.issue=7&rft.spage=2600&rft.epage=2604&rft.pages=2600-2604&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.89.7.2600&rft_dat=%3Cjstor_proqu%3E2358939%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201350385&rft_id=info:pmid/1557364&rft_jstor_id=2358939&rfr_iscdi=true