The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs
Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-02, Vol.89 (4), p.1403-1407 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1407 |
|---|---|
| container_issue | 4 |
| container_start_page | 1403 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 89 |
| creator | Takagaki, Yoshio MacDonald, Clinton C. Shenk, Thomas Manley, James L. |
| description | Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long α-helix stabilized by salt bridges is predicted. An ≈270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (≈20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs. |
| doi_str_mv | 10.1073/pnas.89.4.1403 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_jstor_primary_2358793</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2358793</jstor_id><sourcerecordid>2358793</sourcerecordid><originalsourceid>FETCH-LOGICAL-c637t-ac48cdd6af27bef024095bebb9cec4f28f7983ff07ff38a80257ace9992da75b3</originalsourceid><addsrcrecordid>eNqFkktvEzEUhUcIVNLAlhVIFkLdTfBrxrbUTUgpRSoPVena8njsxsGxw9hTNeLPM1FCGjaw8uJ851zfq1MUrxCcIMjI-3VQacLFhE4QheRJMUJQoLKmAj4tRhBiVnKK6fPiNKUlhFBUHJ4UJ4hRREQ9Kn7NFwZc9SsVQE3LHxcKfI9-o1oTNn7jVXYxgEulc-zALIasXEhAgRvXxNBrb-K6i9m4UM43awNuvk7BBxdaF-7ARVwNMFChBbehT73yYNo_OO9UtwFfYnY2vSieWeWTebl_x8Xt5cf57Kq8_vbp82x6XeqasFwqTblu21pZzBpjIabDFo1pGqGNphZzywQn1kJmLeGKQ1wxpY0QAreKVQ0ZF-e73HXfrEyrTcid8nLdudXwGRmVk38rwS3kXbyXlNNKDPa3O3tM2cmkXTZ6oWMIRmdZUY4pYwN0tp_RxZ-9SVmuXNLGexVM7JNkmCNKMPkviGpEYMXw49gDuIx9F4ZDSQwRrtGWHBeTHaS7mFJn7GErBOW2H3LbD8mFpHLbj8Hw5vgWj_iuEIP-bq-rpJW3nQrapQNWoQHExzHb-D_q8Zizf-nS9t5n85AH8PUOXKahYwcSk4ozQchvaerl2w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201261161</pqid></control><display><type>article</type><title>The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Takagaki, Yoshio ; MacDonald, Clinton C. ; Shenk, Thomas ; Manley, James L.</creator><creatorcontrib>Takagaki, Yoshio ; MacDonald, Clinton C. ; Shenk, Thomas ; Manley, James L.</creatorcontrib><description>Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long α-helix stabilized by salt bridges is predicted. An ≈270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (≈20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.4.1403</identifier><identifier>PMID: 1741396</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture ; AMINES ; AMINO ACID SEQUENCE ; AMINO ACIDS ; ANIMALS ; AZOLES ; BACTERIA ; Base Sequence ; Binding Sites ; Biochemistry ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; Bridge engineering ; CARBOXYLIC ACIDS ; CHEMICAL REACTIONS ; Cloning, Molecular ; CODONS ; Complementary DNA ; CROSS-LINKING ; Deoxyribonucleic acid ; DNA ; DNA - genetics ; DNA SEQUENCING ; ELECTROMAGNETIC RADIATION ; ESCHERICHIA COLI ; Fundamental and applied biological sciences. Psychology ; Gels ; genes ; GLYCINE ; HeLa Cells ; HETEROCYCLIC ACIDS ; HETEROCYCLIC COMPOUNDS ; Humans ; MAMMALS ; MAN ; Messenger RNA ; MICROORGANISMS ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; MOLECULAR STRUCTURE ; Molecular Weight ; mRNA Cleavage and Polyadenylation Factors ; NUCLEIC ACIDS ; NUCLEOPROTEINS ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; Poly A - metabolism ; POLYMERIZATION ; PRIMATES ; PROLINE ; PROTEINS ; PYRROLES ; PYRROLIDINES ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RECOMBINANT DNA ; Ribonucleic acid ; ribonucleoproteins ; Ribonucleoproteins - chemistry ; RNA ; RNA Processing, Post-Transcriptional ; RNA-Binding Proteins - chemistry ; Salts ; STRUCTURAL CHEMICAL ANALYSIS ; Transcription. Transcription factor. Splicing. Rna processing ; ULTRAVIOLET RADIATION ; VERTEBRATES</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-02, Vol.89 (4), p.1403-1407</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Feb 15, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c637t-ac48cdd6af27bef024095bebb9cec4f28f7983ff07ff38a80257ace9992da75b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2358793$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2358793$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5117423$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1741396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5482477$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Takagaki, Yoshio</creatorcontrib><creatorcontrib>MacDonald, Clinton C.</creatorcontrib><creatorcontrib>Shenk, Thomas</creatorcontrib><creatorcontrib>Manley, James L.</creatorcontrib><title>The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long α-helix stabilized by salt bridges is predicted. An ≈270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (≈20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs.</description><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><subject>AMINES</subject><subject>AMINO ACID SEQUENCE</subject><subject>AMINO ACIDS</subject><subject>ANIMALS</subject><subject>AZOLES</subject><subject>BACTERIA</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>Bridge engineering</subject><subject>CARBOXYLIC ACIDS</subject><subject>CHEMICAL REACTIONS</subject><subject>Cloning, Molecular</subject><subject>CODONS</subject><subject>Complementary DNA</subject><subject>CROSS-LINKING</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA SEQUENCING</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>ESCHERICHIA COLI</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>genes</subject><subject>GLYCINE</subject><subject>HeLa Cells</subject><subject>HETEROCYCLIC ACIDS</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>Humans</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Messenger RNA</subject><subject>MICROORGANISMS</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>MOLECULAR STRUCTURE</subject><subject>Molecular Weight</subject><subject>mRNA Cleavage and Polyadenylation Factors</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOPROTEINS</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>Poly A - metabolism</subject><subject>POLYMERIZATION</subject><subject>PRIMATES</subject><subject>PROLINE</subject><subject>PROTEINS</subject><subject>PYRROLES</subject><subject>PYRROLIDINES</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RECOMBINANT DNA</subject><subject>Ribonucleic acid</subject><subject>ribonucleoproteins</subject><subject>Ribonucleoproteins - chemistry</subject><subject>RNA</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA-Binding Proteins - chemistry</subject><subject>Salts</subject><subject>STRUCTURAL CHEMICAL ANALYSIS</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>ULTRAVIOLET RADIATION</subject><subject>VERTEBRATES</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktvEzEUhUcIVNLAlhVIFkLdTfBrxrbUTUgpRSoPVena8njsxsGxw9hTNeLPM1FCGjaw8uJ851zfq1MUrxCcIMjI-3VQacLFhE4QheRJMUJQoLKmAj4tRhBiVnKK6fPiNKUlhFBUHJ4UJ4hRREQ9Kn7NFwZc9SsVQE3LHxcKfI9-o1oTNn7jVXYxgEulc-zALIasXEhAgRvXxNBrb-K6i9m4UM43awNuvk7BBxdaF-7ARVwNMFChBbehT73yYNo_OO9UtwFfYnY2vSieWeWTebl_x8Xt5cf57Kq8_vbp82x6XeqasFwqTblu21pZzBpjIabDFo1pGqGNphZzywQn1kJmLeGKQ1wxpY0QAreKVQ0ZF-e73HXfrEyrTcid8nLdudXwGRmVk38rwS3kXbyXlNNKDPa3O3tM2cmkXTZ6oWMIRmdZUY4pYwN0tp_RxZ-9SVmuXNLGexVM7JNkmCNKMPkviGpEYMXw49gDuIx9F4ZDSQwRrtGWHBeTHaS7mFJn7GErBOW2H3LbD8mFpHLbj8Hw5vgWj_iuEIP-bq-rpJW3nQrapQNWoQHExzHb-D_q8Zizf-nS9t5n85AH8PUOXKahYwcSk4ozQchvaerl2w</recordid><startdate>19920215</startdate><enddate>19920215</enddate><creator>Takagaki, Yoshio</creator><creator>MacDonald, Clinton C.</creator><creator>Shenk, Thomas</creator><creator>Manley, James L.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T3</scope><scope>M81</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19920215</creationdate><title>The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs</title><author>Takagaki, Yoshio ; MacDonald, Clinton C. ; Shenk, Thomas ; Manley, James L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637t-ac48cdd6af27bef024095bebb9cec4f28f7983ff07ff38a80257ace9992da75b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</topic><topic>AMINES</topic><topic>AMINO ACID SEQUENCE</topic><topic>AMINO ACIDS</topic><topic>ANIMALS</topic><topic>AZOLES</topic><topic>BACTERIA</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>Bridge engineering</topic><topic>CARBOXYLIC ACIDS</topic><topic>CHEMICAL REACTIONS</topic><topic>Cloning, Molecular</topic><topic>CODONS</topic><topic>Complementary DNA</topic><topic>CROSS-LINKING</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA SEQUENCING</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>ESCHERICHIA COLI</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>genes</topic><topic>GLYCINE</topic><topic>HeLa Cells</topic><topic>HETEROCYCLIC ACIDS</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>Humans</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Messenger RNA</topic><topic>MICROORGANISMS</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>MOLECULAR STRUCTURE</topic><topic>Molecular Weight</topic><topic>mRNA Cleavage and Polyadenylation Factors</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOPROTEINS</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>Poly A - metabolism</topic><topic>POLYMERIZATION</topic><topic>PRIMATES</topic><topic>PROLINE</topic><topic>PROTEINS</topic><topic>PYRROLES</topic><topic>PYRROLIDINES</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RECOMBINANT DNA</topic><topic>Ribonucleic acid</topic><topic>ribonucleoproteins</topic><topic>Ribonucleoproteins - chemistry</topic><topic>RNA</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA-Binding Proteins - chemistry</topic><topic>Salts</topic><topic>STRUCTURAL CHEMICAL ANALYSIS</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>ULTRAVIOLET RADIATION</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takagaki, Yoshio</creatorcontrib><creatorcontrib>MacDonald, Clinton C.</creatorcontrib><creatorcontrib>Shenk, Thomas</creatorcontrib><creatorcontrib>Manley, James L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Human Genome Abstracts</collection><collection>Biochemistry Abstracts 3</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takagaki, Yoshio</au><au>MacDonald, Clinton C.</au><au>Shenk, Thomas</au><au>Manley, James L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-02-15</date><risdate>1992</risdate><volume>89</volume><issue>4</issue><spage>1403</spage><epage>1407</epage><pages>1403-1407</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long α-helix stabilized by salt bridges is predicted. An ≈270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (≈20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1741396</pmid><doi>10.1073/pnas.89.4.1403</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-02, Vol.89 (4), p.1403-1407 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_2358793 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture AMINES AMINO ACID SEQUENCE AMINO ACIDS ANIMALS AZOLES BACTERIA Base Sequence Binding Sites Biochemistry Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS Bridge engineering CARBOXYLIC ACIDS CHEMICAL REACTIONS Cloning, Molecular CODONS Complementary DNA CROSS-LINKING Deoxyribonucleic acid DNA DNA - genetics DNA SEQUENCING ELECTROMAGNETIC RADIATION ESCHERICHIA COLI Fundamental and applied biological sciences. Psychology Gels genes GLYCINE HeLa Cells HETEROCYCLIC ACIDS HETEROCYCLIC COMPOUNDS Humans MAMMALS MAN Messenger RNA MICROORGANISMS Molecular and cellular biology Molecular genetics Molecular Sequence Data MOLECULAR STRUCTURE Molecular Weight mRNA Cleavage and Polyadenylation Factors NUCLEIC ACIDS NUCLEOPROTEINS ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS Poly A - metabolism POLYMERIZATION PRIMATES PROLINE PROTEINS PYRROLES PYRROLIDINES RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RECOMBINANT DNA Ribonucleic acid ribonucleoproteins Ribonucleoproteins - chemistry RNA RNA Processing, Post-Transcriptional RNA-Binding Proteins - chemistry Salts STRUCTURAL CHEMICAL ANALYSIS Transcription. Transcription factor. Splicing. Rna processing ULTRAVIOLET RADIATION VERTEBRATES |
| title | The Human 64-kDa Polyadenylylation Factor Contains a Ribonucleoprotein-Type RNA Binding Domain and Unusual Auxiliary Motifs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T09%3A03%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Human%2064-kDa%20Polyadenylylation%20Factor%20Contains%20a%20Ribonucleoprotein-Type%20RNA%20Binding%20Domain%20and%20Unusual%20Auxiliary%20Motifs&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Takagaki,%20Yoshio&rft.date=1992-02-15&rft.volume=89&rft.issue=4&rft.spage=1403&rft.epage=1407&rft.pages=1403-1407&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.89.4.1403&rft_dat=%3Cjstor_pnas_%3E2358793%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201261161&rft_id=info:pmid/1741396&rft_jstor_id=2358793&rfr_iscdi=true |